Predominance of oleanane-type ginsenoside R0 and malonyl esters of protopanaxadiol-type ginsenosides in the 20-year-old suspension cell culture of Panax japonicus C.A. Meyer

Глаголева, Е.С.; Konstantinova, Svetlana V.; Кочкин, Д. В.; Ossipov, Vladimir; Titova, M. V.; Попова, Елена; Носов, А. М.; Paek, Kee-Yoeup

doi:10.1016/j.indcrop.2021.114417

Cited by 12 publications

(7 citation statements)

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“…The composition of ginsenosides in cells was evaluated at the time point of achieving maximum biomass concentration for each line, based on previous observations that in the cell cultures of Panax spp., maximum accumulation of ginsenosides usually coincides with maximum biomass gain ( Kochan and Chmiel, 2011 ; Le et al., 2019 ). For example, in Panax japonicus cell culture, the total content of ginsenosides peaked at late-exponential and stationary growth phases, corresponding to the lowest cell proliferative activity, cell elongation, and nuclear DNA reduplication ( Smolenskaya et al., 2007a ; Glagoleva et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…This ginsenoside is found predominantly in P. japonicus ( Han et al., 2005 ; Wang et al., 2021 ), whereas its presence in P. vietnamensis has not been previously reported. It is also interesting that none of the cell cultures contained ginsenosides of the protopanaxatriol group that are often found in plants and plant cell cultures of various ginseng species ( Nguyen et al., 1993 , Nguyen et al., 2007 ; Le et al., 2018 ; Glagoleva et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

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Suspension cell cultures of Panax vietnamensis as a biotechnological source of ginsenosides: growth, cytology, and ginsenoside profile assessment

Titova,

Lunkova,

Tyurina

et al. 2024

Front. Plant Sci.

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IntroductionPanax vietnamensis is a valuable medicinal plant and a source of a broad spectrum of biologically active ginsenosides of different structural groups. Overexploitation and low adaptability to planation cultivation have made this species vulnerable to human pressure and prompted the development of cell cultivation in vitro as a sustainable alternative to harvesting wild plants for their bioactive components. Despite high interest in biotechnological production, little is known about the main factors affecting cell growth and ginsenoside biosynthesis of this species under in vitro conditions. In this study, the potential of cell cultures of P. vietnamensis as a biotechnological source of ginsenosides was was assessed.MethodsSix suspension cell lines that were developed from different sections of a single rhizome through a multi-step culture optimization process and maintained for over 3 years on media with different mineral salt base and varying contents of auxins and cytokinins. These cell lines were evaluated for productivity parameters and cytological characteristics. Ginsenoside profiles were assessed using a combination of the reversed-phase ultra-high-performance liquid chromatography–Orbitrap–tandem mass spectrometry (UHPLC–Orbitrap–MS/MS) and ultra-performance liquid chromatography–time of flight–mass spectrometry (UPLC–TOF–MS).ResultsAll lines demonstrated good growth with a specific growth rate of 0.1–0.2 day−1, economic coefficient of 0.31–0.70, productivity on dry weight (DW) of 0.30–0.83 gDW (L·day)−1, and maximum biomass accumulation varying from 10 to 22 gDW L−1. Ginsenosides of the protopanaxadiol (Rb1, Rb2/Rb3, malonyl-Rb1, and malonyl-Rb2/Rb3), oleanolic acid (R0 and chikusetsusaponin IV), and ocotillol (vinaginsenoside R1) groups and their isomers were identified in cell biomass extracts. Chikusetsusaponin IV was identified in P. vietnamensis cell culture for the first time.DiscussionThese results suggest that suspension cell cultures of Vietnamese ginseng have a high potential for the biotechnological production of biomass containing ginsenosides, particularly of the oleanolic acid and ocotillol groups.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Suspension cell cultures of Panax vietnamensis as a biotechnological source of ginsenosides: growth, cytology, and ginsenoside profile assessment

Titova,

Lunkova,

Tyurina

et al. 2024

Front. Plant Sci.

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“…The ratio of furostanol and spirostanol-type glycosides differed between the plant rhizomes and several cell culture strains of Dioscorea deltoidea [ 72 ]. The cell culture of Panax japonicus used in the present study accumulated high levels of “acidic” ginsenosides (malonyl derivatives of dammarane ginsenosides and glycosides of oleanolic acid) in contrast to the plant roots where neutral ginsenosides are usually prevailing [ 73 ]. Phenylethanoids are major compounds in the cell culture of Tribulus terrestris , but only trace amounts of these chemicals are usually found in plants [ 74 ].…”

Section: Discussionmentioning

confidence: 99%

The Hypoglycemic and Hypocholesterolemic Activity of Dioscorea deltoidea, Tribulus terrestris and Panax japonicus Cell Culture Biomass in Rats with High-Fat Diet-Induced Obesity

et al. 2023

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Obesity, and its consequences for human health, is a huge and complicated problem that has no simple solution. The constant search for natural and safe compounds with systemic action that can be used for obesity prophylactics and treatment is hampered by the limited availability and variable quality of biomass of wild medicinal plants. Plant cell biotechnology is an alternative approach for the sustainable production of vegetative biomass or individual phytochemicals with high therapeutic potential. In this study, the suspension cell biomass of the medicinal plants, Dioscorea deltoidea Wall., Tribulus terrestris L., and Panax japonicus (T. Nees) C.A. Mey, produced in 20 L and 630 L bioreactors, were tested for therapeutic effects in rat models with alimentary-induced obesity. Three-month intake of water infusions of dry cell biomass (100 mg/g body weight) against the background of a hypercaloric diet reduced weight gain and the proportion of fat mass in the obese animals. In addition, cell biomass preparation reduced the intracellular dehydration and balanced the amounts of intra- and extracellular fluids in the body as determined by bioimpedance spectroscopy. A significant decrease in the glucose and cholesterol levels in the blood was also observed as a result of cell biomass administration for all species. Hypocholesterolemic activity reduced in the line P. japonicus > D. deltoidea > T. terrestris/liraglutide > intact group > control group. By the sum of parameters tested, the cell culture of D. deltoidea was considered the most effective in mitigating diet-induced obesity, with positive effects sometimes exceeding those of the reference drug liraglutide. A safety assessment of D. deltoidea cell phytopreparation showed no toxic effect on the reproductive function of the animals and their offspring. These results support the potential application of the biotechnologically produced cell biomass of medicinal plant species as safe and effective natural remedies for the treatment of obesity and related complications, particularly for the long-term treatment and during pregnancy and lactation periods when conventional treatment is often contraindicated.

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“…The cell culture collection at IPPRAS, with its long-term cultured strains, is well-positioned to be utilized in experiments exploring stability monitoring in cell cultures of different taxa. For example, the cell suspension of Panax japonicus maintained by periodic subcultures for over 20 years fully retained its growth characteristics [ 30 ] and produced a broad spectrum of ginsenosides, including protopanaxatriols (Re, Rg 1 , Rf), protopanaxadiols (Rb 1 , Rb 2 , Rc, Rd), ginsenoside R 0 , and malonyl-ginsenosides [ 69 ]. The main growth parameters recorded during cultivation in the 20-L, 75-L, and 630-L bioreactors remained unchanged for the suspension cell culture of Polyscias filicifolia after five years of maintenance by periodic subcultures [ 70 ].…”

Section: All-russian Collection Of Plant Cell Culturesmentioning

confidence: 99%

Plants, Cells, Algae, and Cyanobacteria In Vitro and Cryobank Collections at the Institute of Plant Physiology, Russian Academy of Sciences—A Platform for Research and Production Center

Yuorieva,

Sinetova,

Messineva

et al. 2023

Biology

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Ex situ collections of algae, cyanobacteria, and plant materials (cell cultures, hairy and adventitious root cultures, shoots, etc.) maintained in vitro or in liquid nitrogen (−196 °C, LN) are valuable sources of strains with unique ecological and biotechnological traits. Such collections play a vital role in bioresource conservation, science, and industry development but are rarely covered in publications. Here, we provide an overview of five genetic collections maintained at the Institute of Plant Physiology of the Russian Academy of Sciences (IPPRAS) since the 1950–1970s using in vitro and cryopreservation approaches. These collections represent different levels of plant organization, from individual cells (cell culture collection) to organs (hairy and adventitious root cultures, shoot apices) to in vitro plants. The total collection holdings comprise more than 430 strains of algae and cyanobacteria, over 200 potato clones, 117 cell cultures, and 50 strains of hairy and adventitious root cultures of medicinal and model plant species. The IPPRAS plant cryobank preserves in LN over 1000 specimens of in vitro cultures and seeds of wild and cultivated plants belonging to 457 species and 74 families. Several algae and plant cell culture strains have been adapted for cultivation in bioreactors from laboratory (5–20-L) to pilot (75-L) to semi-industrial (150–630-L) scale for the production of biomass with high nutritive or pharmacological value. Some of the strains with proven biological activities are currently used to produce cosmetics and food supplements. Here, we provide an overview of the current collections’ composition and major activities, their use in research, biotechnology, and commercial application. We also highlight the most interesting studies performed with collection strains and discuss strategies for the collections’ future development and exploitation in view of current trends in biotechnology and genetic resources conservation.

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Predominance of oleanane-type ginsenoside R0 and malonyl esters of protopanaxadiol-type ginsenosides in the 20-year-old suspension cell culture of Panax japonicus C.A. Meyer

Cited by 12 publications

References 59 publications

Suspension cell cultures of Panax vietnamensis as a biotechnological source of ginsenosides: growth, cytology, and ginsenoside profile assessment

Suspension cell cultures of Panax vietnamensis as a biotechnological source of ginsenosides: growth, cytology, and ginsenoside profile assessment

The Hypoglycemic and Hypocholesterolemic Activity of Dioscorea deltoidea, Tribulus terrestris and Panax japonicus Cell Culture Biomass in Rats with High-Fat Diet-Induced Obesity

Plants, Cells, Algae, and Cyanobacteria In Vitro and Cryobank Collections at the Institute of Plant Physiology, Russian Academy of Sciences—A Platform for Research and Production Center

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