Predominance of ST5-II-t311 clone among healthcare-associated methicillin-resistant Staphylococcus aureus isolates recovered from Zhejiang, China

Wu, Dang; Wang, Zhengan; Wang, Haiping; Sun, Lu; Chen, Yan; Ji, Shujuan; Shi, Kezhang; Yu, Yunsong

doi:10.1016/j.ijid.2018.04.798

Cited by 20 publications

(19 citation statements)

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“…Egyir et al [48] reported this clone as one of the predominant clones among healthcare institutions in Ghana. It is also reported as one of the predominant clones causing blood stream infections in Europe [58,59], Asia [60,61] and South America [62].…”

Section: Source (N)mentioning

confidence: 99%

Molecular epidemiology, genetic diversity and antimicrobial resistance of Staphylococcus aureus isolated from chicken and pig carcasses, and carcass handlers

et al. 2020

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The epidemiology of Staphylococcus aureus in food animals, associated products, and their zoonotic potential in Nigeria are poorly understood. This study aimed to provide data on the prevalence, genetic characteristics and antimicrobial resistance of S. aureus isolated from chicken and pig carcasses, and persons in contact with the carcasses at slaughterhouses in Nigeria. Surface swabs were collected randomly from 600 chicken and 600 pig carcasses. Nasal swabs were collected from 45 workers in chicken slaughterhouses and 45 pig slaughterhouse workers. S. aureus isolates were analyzed by spa typing. They were also examined for presence of the Panton-Valentine Leucocidin (PVL) and mecA genes, as well as for antimicrobial resistance phenotype. Overall, 53 S. aureus isolates were recovered (28 from chicken carcasses, 17 from pig carcasses, 5 from chicken carcass handlers and 3 from pig carcass handlers). Among the isolates, 19 (35.8%) were PVL-positive and 12 (22.6%) carried the mecA gene. The 53 isolates belonged to 19 spa types. The Based Upon Repeat Pattern (BURP) algorithm separated the isolates into 2 spa-clonal complexes (spa-CC) and 9 singletons including 2 novel spa types (t18345 and t18346). The clonal complexes (CC) detected were CC1, CC5, CC8, CC15, CC88 and CC152. CC15-related isolates represented by spa type t084 (32.1%) and CC5 represented by spa type t311 (35.3%) predominated among isolates from chicken carcasses/ handlers, and pig carcasses/ handlers, respectively. Multidrug resistance exhibited by all the CC except CC8, was observed among isolates from chicken carcasses (64.3%), pig carcasses (41.2%), handlers of chicken meat (40.0%) and handlers of pork (33.3%). All the CC showed varying degrees of resistance to tetracycline while CC15 and CC5 exhibited the highest resistance to sulphamethoxazole/trimethoprim and erythromycin, respectively. The predominant antimicrobial resistance

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Section: Source (N)mentioning

confidence: 99%

Molecular epidemiology, genetic diversity and antimicrobial resistance of Staphylococcus aureus isolated from chicken and pig carcasses, and carcass handlers

et al. 2020

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“…ST59 was a major CA-MRSA and the second common MRSA in ours results. Our nding indicated the distinctions between CA-MRSA and HA-MRSA isolates have become blurred and they could be a mutual integration 29 . Previous studies indicated that ST59 MRSA might spread into hospital from community 29,33 .…”

Section: Discussionmentioning

confidence: 79%

“…ST5-II MRSA (NEW York/Japan) clone was one of the most predominant genotypes in many countries 27,28 . Also, previous study showed that ST5 was the predominant HA-MRSA clone in Zhejiang, China from 2012 to 2013 29 . Moreover, some investigators have expressed different opinions on the most main spa type of ST5.…”

Section: Discussionmentioning

confidence: 94%

Molecular Characteristics and Changing Trend of Methicillin-Resistant Staphylococcus Aureus From Invasive Infections between 2012 and 2018

Xiao

Zhu

Wang

et al. 2020

Preprint

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Background Methicillin-resistant Staphylococcus aureus (MRSA) is a major global problem. The analysis of the molecular characteristics and changing trend of MRSA is essential for the control and treatment of diseases caused by the pathogen.Methods A total of 162 MRSA isolates from invasive infections between 2012 and 2018 were collected, molecular typing and antimicrobial susceptibility tests to explore its molecular epidemiologic change in a hospital were performed.Results All of the 162 MRSA isolates (86.4% HA-MRSA and 13.6% CA-MRSA) were divided into 16 different ST and 30 Spa types. The major STs were ST5 (96/162, 59.3%) and the predominant spa type was t311(83/162, 51.2%). Five SCCmec types were found and the most common SCCmec type was type II (101/162, 61.7%). The prevalence of ST5 MRSA gradually declined from 2014 to 2018 but the prevalence of ST59 MRSA significantly increased. At the same time, livestock-associated methicillin-resistant S.aureus ST239 and ST9 were detected. 28 isolates were Panton-valentine leucocidin (pvl) gene positive (28/162, 17.3%). The most prevalent pvl-positive clone was ST59-IVa-t437. Comparing with HA-MRSA, CA-MRSA had a lower probability of ST5 (9.1% vs, 67.1%, P=0.000) but a higher probability of ST59 (63.6% vs. 11.4%, P=0.000), not only that, it was more likely to carrying pvl-positive gene (36.4% vs. 14.3%, P=0.028).Conclusions The molecular types of MRSA were getting complex over time. ST5-II-t311 was the predominant clone of MRSA isolate with a downward incidence from 2012 to 2018. ST59 MRSA strains, which is thought community related strain are spreading into hospitals and has an upward incidence during the investigational period.

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“…Zhejiang, Guangdong, and Guangxi [15][16][17][18]. To date, however, no study has focused on the molecular characteristics and virulence gene profiles of S. aureus isolates in Hainan, and no hospital in Hainan has been included in any multi-center studies concerned with those characteristics of S. aureus in China [13,19,20].…”

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confidence: 99%

Molecular characteristics and virulence gene profiles of Staphylococcus aureus isolates in Hainan, China

Huang

et al. 2019

Preprint

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Background: There have been no reports regarding the molecular characteristics, virulence features, and antibiotic resistance profiles of Staphylococcus aureus ( S. aureus ) from Hainan, the southernmost province of China. Methods: 227 S. aureus isolates, consisting of 76 methicillin-resistant S. aureus (MRSA) and 151 methicillin-susceptible S. aureus (MSSA), were collected in 2013-2014 and 2018-2019 in Hainan, and investigated for their molecular characteristics, virulence genes, antibiotic resistance profiles and main antibiotic resistance genes. Results: Forty sequence types (STs) including three new STs (ST5489, ST5492 and ST5493), and 79 Staphylococcal protein A ( spa ) types were identified based on multilocus sequence typing (MLST) and spa typing, respectively. ST398 (14.1%, 32/227) was found to be the most prevalent, and the prevalence of ST398-MSSA increased significantly from 2013-2014 (5.5%, 5/91) to 2018-2019 (18.4%, 25/136). Seventy-six MRSA isolates were subject to staphylococcus chromosomal cassette mec (SCC mec ) typing. SCC mec- IVa was the predominant SCC mec type, and specifically, ST45-SCC mec IVa, an infrequent type in mainland China, was predominant in S. aureus from Hainan. The antibiotic resistance profiles and antibiotic resistance genes of S . aureus show distinctive features in Hainan. The resistant rates of the MRSA isolates to a variety of antibiotics were significantly higher than those of the MSSA isolates. The predominant erythromycin and tetracycline resistance genes were ermC (90.1%, 100/111) and tetK (91.8%, 78/85), respectively. Eleven virulence genes, including the Panton-Valentine leukocidin ( pvl ) and eta , were determined, and the frequency of eta and pvl were found to be 57.3% and 47.6%. Such high prevalence has never been seen in mainland China before. Conclusion: S. aureus isolates in Hainan have unique molecular characteristics, virulence gene and antibiotic resistance profiles, and main antibiotic resistance genes which may be associated with the special geographical location of Hainan and local trends in antibiotic use.

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Predominance of ST5-II-t311 clone among healthcare-associated methicillin-resistant Staphylococcus aureus isolates recovered from Zhejiang, China

Abstract: CC5/ST5-MRSA-II-t311 has become the predominant HA-MRSA clone in Hangzhou, Zhejiang Province. ST59 MRSA has spread into hospitals. The isolates showed good susceptibility to all five anti-MRSA agents.

Cited by 20 publications

References 35 publications

Molecular epidemiology, genetic diversity and antimicrobial resistance of Staphylococcus aureus isolated from chicken and pig carcasses, and carcass handlers

Molecular epidemiology, genetic diversity and antimicrobial resistance of Staphylococcus aureus isolated from chicken and pig carcasses, and carcass handlers

Molecular Characteristics and Changing Trend of Methicillin-Resistant Staphylococcus Aureus From Invasive Infections between 2012 and 2018

Molecular characteristics and virulence gene profiles of Staphylococcus aureus isolates in Hainan, China

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Predominance of ST5-II-t311 clone among healthcare-associated methicillin-resistant Staphylococcus aureus isolates recovered from Zhejiang, China

Abstract: CC5/ST5-MRSA-II-t311 has become the predominant HA-MRSA clone in Hangzhou, Zhejiang Province. ST59 MRSA has spread into hospitals. The isolates showed good susceptibility to all five anti-MRSA agents.

Cited by 20 publications

References 35 publications

Molecular epidemiology, genetic diversity and antimicrobial resistance of Staphylococcus aureus isolated from chicken and pig carcasses, and carcass handlers

Molecular epidemiology, genetic diversity and antimicrobial resistance of Staphylococcus aureus isolated from chicken and pig carcasses, and carcass handlers

Molecular Characteristics and Changing&nbsp;Trend&nbsp;of Methicillin-Resistant Staphylococcus Aureus&nbsp;From&nbsp;Invasive&nbsp;Infections between&nbsp;2012 and 2018

Molecular characteristics and virulence gene profiles of Staphylococcus aureus isolates in Hainan, China

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Molecular Characteristics and Changing Trend of Methicillin-Resistant Staphylococcus Aureus From Invasive Infections between 2012 and 2018