Predominance of ST8 and CC1/spa-t1784 methicillin-resistant Staphylococcus aureus isolates in Japan and their genomic characteristics

Ogura, Kohei; Kaji, Daiki; Sasaki, Masakazu; Otsuka, Yoshihito; Takemoto, Norihiko; Miyoshi‐Akiyama, Tohru; Kikuchi, Ken

doi:10.1016/j.jgar.2022.01.011

Cited by 12 publications

(7 citation statements)

References 32 publications

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“…However, with the increasing prevalence of HA-MRSA in different countries, MRSA prevalence also noted lower in many such as 4.6% from Germany (Sassmannshausen et al, 2016), 25% from Texas (Davis et al, 2004), 19.1% from Mexico (Hamdan-Partida et al, 2022, 15.1% from Australia (Coombs et al, 2022), and 26% from Italy (La Vecchia et al, 2022) are summarized in Table 1. Similar increasing and decreasing trend of MRSA prevalence from different countries also noted for CA-MRSA such as 79% from Japan (Ogura et al, 2022), 84.9% from Australia (Coombs et al, 2022), 64.7% from India (Alvarez-Uria and Reddy, 2012), 61% from Norway (Enger et al, 2022), and 44.3% from Iran (Tabandeh et al, 2022) with lower prevalence from Egypt (16%) (Mostafa et al, 2022), China [1.7% (Bi et al, 2018); 24% (Chen et al, 2022)], 7.3% from Gerorgia (Hidron et al, 2005), and 12.8% from Switzerland (Harbarth et al, 2005). This decline in the prevalence of HA-MRSA and CA-MRSA may be linked with better implementation national prevention measures.…”

Section: Prevalence Of Mrsasupporting

confidence: 74%

MRSA compendium of epidemiology, transmission, pathophysiology, treatment, and prevention within one health framework

et al. 2023

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Staphylococcus aureus is recognized as commensal as well as opportunistic pathogen of humans and animals. Methicillin resistant strain of S. aureus (MRSA) has emerged as a major pathogen in hospitals, community and veterinary settings that compromises the public health and livestock production. MRSA basically emerged from MSSA after acquiring SCCmec element through gene transfer containing mecA gene responsible for encoding PBP-2α. This protein renders the MRSA resistant to most of the β-lactam antibiotics. Due to the continuous increasing prevalence and transmission of MRSA in hospitals, community and veterinary settings posing a major threat to public health. Furthermore, high pathogenicity of MRSA due to a number of virulence factors produced by S. aureus along with antibiotic resistance help to breach the immunity of host and responsible for causing severe infections in humans and animals. The clinical manifestations of MRSA consist of skin and soft tissues infection to bacteremia, septicemia, toxic shock, and scalded skin syndrome. Moreover, due to the increasing resistance of MRSA to number of antibiotics, there is need to approach alternatives ways to overcome economic as well as human losses. This review is going to discuss various aspects of MRSA starting from emergence, transmission, epidemiology, pathophysiology, disease patterns in hosts, novel treatment, and control strategies.

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Section: Prevalence Of Mrsasupporting

confidence: 74%

MRSA compendium of epidemiology, transmission, pathophysiology, treatment, and prevention within one health framework

et al. 2023

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“…More important CA-MRSA clones according to [ 2 , 23 , 217 , 218 , 219 , 220 , 221 , 222 , 223 , 224 , 225 , 226 , 227 , 228 , 229 , 230 , 231 , 232 ] are shown in Table 4 , most important HA-MRSA clones according to [ 23 , 85 , 125 , 220 , 224 , 233 , 234 , 235 , 236 ] are shown in Table 5 , and most important LA-MRSA clones according to [ 23 , 125 , 237 , 238 , 239 , 240 , 241 , 242 ] are shown in Table 6 .…”

Section: Molecular Epidemiology Of Mrsamentioning

confidence: 99%

Molecular Mechanisms of Drug Resistance in Staphylococcus aureus

Młynarczyk-Bonikowska

Kowaléwski

Krolak-Ulinska³

et al. 2022

IJMS

151

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This paper discusses the mechanisms of S. aureus drug resistance including: (1) introduction. (2) resistance to beta-lactam antibiotics, with particular emphasis on the mec genes found in the Staphylococcaceae family, the structure and occurrence of SCCmec cassettes, as well as differences in the presence of some virulence genes and its expression in major epidemiological types and clones of HA-MRSA, CA-MRSA, and LA-MRSA strains. Other mechanisms of resistance to beta-lactam antibiotics will also be discussed, such as mutations in the gdpP gene, BORSA or MODSA phenotypes, as well as resistance to ceftobiprole and ceftaroline. (3) Resistance to glycopeptides (VRSA, VISA, hVISA strains, vancomycin tolerance). (4) Resistance to oxazolidinones (mutational and enzymatic resistance to linezolid). (5) Resistance to MLS-B (macrolides, lincosamides, ketolides, and streptogramin B). (6) Aminoglycosides and spectinomicin, including resistance genes, their regulation and localization (plasmids, transposons, class I integrons, SCCmec), and types and spectrum of enzymes that inactivate aminoglycosides. (7). Fluoroquinolones (8) Tetracyclines, including the mechanisms of active protection of the drug target site and active efflux of the drug from the bacterial cell. (9) Mupirocin. (10) Fusidic acid. (11) Daptomycin. (12) Resistance to other antibiotics and chemioterapeutics (e.g., streptogramins A, quinupristin/dalfopristin, chloramphenicol, rifampicin, fosfomycin, trimethoprim) (13) Molecular epidemiology of MRSA.

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“…For example, a report on MRSA isolated from 13 hospitals in Tokyo and surrounding prefectures showed that the SCCmec type IV/CC1 clone was most frequently detected and possessed sea. 13 In a neonatal intensive care unit in Shizuoka, 86.0% of MRSA isolates exhibited SCCmec type IV/CC1 and were all resistant to new quinolones. 19 Recently, a regional outbreak of SCCmec type IV/CC1 (ST2725) MRSA was reported in Niigata.…”

Section: Discussionmentioning

confidence: 99%

“…The USA300 clone (SCCmec type IV/ST8), prevalent in the United States, carries the PVL gene (lukS/F-PV). 10,[13][14][15] Because each clone possesses different and characteristic virulence genes, all MRSA isolates were analyzed for the SCCmec type and screened for the presence of tst, sea, seb, sec, and lukS/F-PV using PCR, as previously reported. 16,17 MLST was performed on all isolates using previously reported primers.…”

Section: Study Definitionsmentioning

confidence: 99%

The clinical characteristics and molecular epidemiology of methicillin‐resistant Staphylococcus aureus (MRSA) among very elderly people in Japan

Mitsumoto‐Kaseida,

Morinaga,

Sasaki

et al. 2023

Geriatrics Gerontology Int

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AimMethicillin‐resistant Staphylococcus aureus (MRSA) is a major nosocomial infection‐causing pathogen. The clonal shift from staphylococcal cassette chromosome mec (SCCmec) type II MRSA to SCCmec type IV MRSA has occurred rapidly in acute‐care hospitals. However, the epidemiology and clinical impacts of MRSA in geriatric hospitals are poorly documented. We performed a molecular epidemiological analysis of the clinical isolates and retrospectively investigated the clinical characteristics of SCCmec type IV MRSA in elderly individuals.MethodsMRSA isolates were grouped according to the SCCmec type and virulence genes (tst, sea, seb, sec, and lukS/F‐PV), and multi‐locus sequence typing (MLST) was performed.ResultsOf the 145 MRSA isolates obtained from patients with a median age of 85 years, 100 (69.0%) were obtained from sputum samples, 22 (15.2%) from skin and soft tissues, and seven (4.8%) from blood samples. The most prevalent clone was SCCmec type IV/clonal complex (CC)1/sea+ (59.3%), followed by SCCmec type I/sequence type (ST) 8 (17.3%). Of the 17 (11.7%) strains to which an anti‐MRSA drug was administered by a physician, only three were SCCmec type IV/CC1/sea+ (17.6%) and five were SCCmec type I/ST8 (29.4%). SCCmec type IV/CC1/sea+ MRSA was more frequently isolated in long‐term care wards than were SCCmec type I/ST8 strains (odds ratio: 2.85, 95% confidence interval: 1.08–7.54) and was less frequently treated as the cause of MRSA infections (odds ratio: 0.15, 95% confidence interval: 0.03–0.73).ConclusionsSCCmec type IV/CC1/sea+ MRSA was the predominant clone and could be easily transmissible and be capable of colonization. Geriatr Gerontol Int 2023; 23: 744–749.

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Predominance of ST8 and CC1/spa-t1784 methicillin-resistant Staphylococcus aureus isolates in Japan and their genomic characteristics

Cited by 12 publications

References 32 publications

MRSA compendium of epidemiology, transmission, pathophysiology, treatment, and prevention within one health framework

MRSA compendium of epidemiology, transmission, pathophysiology, treatment, and prevention within one health framework

Molecular Mechanisms of Drug Resistance in Staphylococcus aureus

The clinical characteristics and molecular epidemiology of methicillin‐resistant Staphylococcus aureus (MRSA) among very elderly people in Japan

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