Predominant expression and cellular distribution of fish Agr2 in renal collecting system

Xia, Jianhong; Jiang, Jun; Shi, Yaohua; Gui, Jian‐Fang

doi:10.1016/j.cbpb.2009.01.014

Cited by 7 publications

(3 citation statements)

References 34 publications

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“…On the other hand, the expression of AGR2 , ADH1C and BCAT1 in the human developing kidney has not been previously reported. CagAgr2 (the fish homolog of AGR2) is expressed in the renal collecting system from gibel carp (Xia et al, 2009) and in the intrahepatic, hilar and extrahepatic biliary tree in embryonic and adult human samples (Lepreux et al, 2011), and Adh1 is expressed in the urothelium of the developing mouse kidney (Mitchell et al, 2006). BCAT1 (branched chain aminotransferase 1) has not been studied in the kidney nor its expression reported; it has been shown to regulate early liver bud growth in the developing mice and in human embryonic stem cell-derived liver organoids (Koike et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Single-cell analysis of progenitor cell dynamics and lineage specification in the human fetal kidney

et al. 2018

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The mammalian kidney develops through reciprocal interactions between the ureteric bud and the metanephric mesenchyme to give rise to the entire collecting system and the nephrons. Most of our knowledge of the developmental regulators driving this process arises from the study of gene expression and functional genetics in mice and other animal models. In order to shed light on human kidney development, we have used single-cell transcriptomics to characterize gene expression in different cell populations, and to study individual cell dynamics and lineage trajectories during development. Single-cell transcriptome analyses of 6414 cells from five individual specimens identified 11 initial clusters of specific renal cell types as defined by their gene expression profile. Further subclustering identifies progenitors, and mature and intermediate stages of differentiation for several renal lineages. Other lineages identified include mesangium, stroma, endothelial and immune cells. Novel markers for these cell types were revealed in the analysis, as were components of key signaling pathways driving renal development in animal models. Altogether, we provide a comprehensive and dynamic gene expression profile of the developing human kidney at the single-cell level.

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Section: Discussionmentioning

confidence: 99%

Single-cell analysis of progenitor cell dynamics and lineage specification in the human fetal kidney

et al. 2018

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“…The proteins have a thioredoxin fold as well as an N-terminal signal sequence targeting them to the secretory pathway (Persson et al 2005). They are widely expressed in vertebrates and are generally found in secretory epithelia (Sive et al 1989;Aberger et al 1998;Kim et al 2007;Kumar et al 2007;Shih et al 2007;Park et al 2009;Xia et al 2009). There is now an extensive literature on upregulation of anterior gradient proteins in mammalian adenocarcinoma (Thompson & Weigel, 1998;Fletcher et al 2003;Barraclough et al 2009) and the premalignant condition called Barrett's oesophagus (Pohler et al 2004;Wang et al 2008).…”

Section: Introductionmentioning

confidence: 99%

A comparative study of gland cells implicated in the nerve dependence of salamander limb regeneration

et al. 2010

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Limb regeneration in salamanders proceeds by formation of the blastema, a mound of proliferating mesenchymal cells surrounded by a wound epithelium. Regeneration by the blastema depends on the presence of regenerating nerves and in earlier work it was shown that axons upregulate the expression of newt anterior gradient (nAG) protein first in Schwann cells of the nerve sheath and second in dermal glands underlying the wound epidermis. The expression of nAG protein after plasmid electroporation was shown to rescue a denervated newt blastema and allow regeneration to the digit stage. We have examined the dermal glands by scanning and transmission electron microscopy combined with immunogold labelling of the nAG protein. It is expressed in secretory granules of ductless glands, which apparently discharge by a holocrine mechanism. No external ducts were observed in the wound epithelium of the newt and axolotl. The larval skin of the axolotl has dermal glands but these are absent under the wound epithelium. The nerve sheath was stained post-amputation in innervated but not denervated blastemas with an antibody to axolotl anterior gradient protein. This antibody reacted with axolotl Leydig cells in the wound epithelium and normal epidermis. Staining was markedly decreased in the wound epithelium after denervation but not in the epidermis. Therefore, in both newt and axolotl the regenerating axons induce nAG protein in the nerve sheath and subsequently the protein is expressed by gland cells, under (newt) or within (axolotl) the wound epithelium, which discharge by a holocrine mechanism. These findings serve to unify the nerve dependence of limb regeneration.

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“…AGR2-203 is the predominant isoform and hereinafter referred to as AGR2, a~19kDa proteins comprising 175 amino acids (Figure 1). Human AGR2 protein is strongly expressed in endoderm-derived organs such as the lung, stomach, colon, prostate and small intestine tissues, and also in organs that contain mucus-secreting cells, or function as endocrine organs [22,23]. In normal tissues, AGR2 can contribute to the regulation of the total protein loads in the cells.…”

Section: Agr2 Expression In Normal and Cancer Conditionmentioning

confidence: 99%

Secretion of pro-oncogenic AGR2 protein in cancer

Moidu¹,

Rahman²,

Syafruddin

et al. 2020

Heliyon

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Anterior gradient-2 (AGR2) protein mediates the formation, breakage and isomerization of disulphide bonds during protein maturation in the endoplasmic reticulum (ER) and contributes to the homoeostasis of the secretory pathway. AGR2 promotes tumour development and metastasis and its elevated expression is almost completely restricted to malignant tumours. Interestingly, this supposedly ER-resident protein can be localised to other compartments of cancer cells and can also be secreted into the extracellular milieu. There are emerging evidences that describe the gain-of-function activities of the extracellular AGR2, particularly in cancer development. Here, we reviewed studies detailing the expression, pathological and physiological roles associated with AGR2 and compared the duality of localization, intracellular and extracellular, with special emphasis on the later. We also discussed the possible mechanisms of AGR2 secretion as well as deliberating the functional impacts of AGR2 in cancer settings. Last, we deliberate the current therapeutic strategies and posit the potential use AGR2, as a prognosis and diagnosis marker in cancer.

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Predominant expression and cellular distribution of fish Agr2 in renal collecting system

Cited by 7 publications

References 34 publications

Single-cell analysis of progenitor cell dynamics and lineage specification in the human fetal kidney

Single-cell analysis of progenitor cell dynamics and lineage specification in the human fetal kidney

A comparative study of gland cells implicated in the nerve dependence of salamander limb regeneration

Secretion of pro-oncogenic AGR2 protein in cancer

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