Prefabrication of axial vascularized tissue engineering coral bone by an arteriovenous loop: A better model

Dong, Qingshan; Shang, Haitao; Wu, Wei; Chen, Fu-lin; Zhang, Junrui; Guo, Jun; Mao, Tianqiu

doi:10.1016/j.msec.2012.04.039

Cited by 21 publications

(23 citation statements)

References 26 publications

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“…Vascular endangium lesions caused lipid deposits to reduce arterial elasticity, after which the narrow cavity induced cardiovascular symptoms (Dong et al, 2012). Thus, lipid deposits may be reduced and vascular elasticity can be recovered.…”

Section: Discussionmentioning

confidence: 99%

Effect of Xin Mai Jia on atherosclerosis in rats

Li¹,

Pan²,

Jia³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. We investigated the therapeutic effect of Xin Mai Jia (XMJ) on atherosclerosis (AS) in rats. Rat models of AS were established by peritoneally injecting vitamin D, feeding a high-fat diet, and inducing balloon injuries in rats. The stomachs of the rats were irrigated continuously for 10 weeks with XMJ. Blood lipid-and hemorheologyrelated indices of blood samples were detected. Pathological changes in the right common carotid arterial tissues were also determined. The protein expression levels of endothelial nitric oxide synthase, angiotensin-1, and endothelin-1 were determined by western blotting. XMJ reduced cholesterol, trigylecride, and low-density lipoprotein levels as well as blood viscosity, sedimentation, and hematocrit. Furthermore, XMJ alleviated vascular endothelial injury and reduced/eliminated atherosclerotic plaques. In contrast, XMJ significantly increased the endothelium-dependent relaxing response of the AS rat models. The Effect of XMJ on atherosclerosis western blotting results showed that XMJ upregulated endothelial nitric oxide synthase but downregulated angiotensin-1 and endothelin-1. XMJ prevented the development of AS by regulating blood lipid levels, hemorheology, and vascular function.

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Section: Discussionmentioning

confidence: 99%

Effect of Xin Mai Jia on atherosclerosis in rats

Li¹,

Pan²,

Jia³

et al. 2015

Genet. Mol. Res.

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“…A lot of different bone substitutes were implanted, including stable [31] or non-stable scaffolds [30,[32][33][34] composed of 60% hydroxyapatite (HA) and 40% β-tricalcium phosphate (β-TCP) [30][31][32][33][34] or 100% β-TCP [35], natural coral scaffolds [36], processed bovine cancellous bone [37], or nanocrystalline HA in a silica gel matrix [29,38]. To functionalize the scaffolds and to support bone formation, cells can be combined with the replacement material, e.g., non-differentiated bone-marrow derived mesenchymal stem cells (MSCs) [30,32,34,39] or fully differentiated osteogenic cells such as osteoblasts [40].…”

Section: The Av Loop As a Tool To Generate A Multitude Of Different Tmentioning

confidence: 99%

“…However, 2 out of 6 animals had to be excluded due to vascular thrombosis. Dong et al [36] also used the rabbit model, but took a different approach to vascularize a construct of 6 × 8 × 10 mm. Instead of using the femoral vascular bundle like for the rat model or the saphenous vascular bundle as described by Eweida et al [53], the popliteal artery was anastomosed with the femoral vein.…”

Section: Animal Modelsmentioning

confidence: 99%

“…To reduce bending of the loop vessels and therefore the risk of thromboses, Zhan et al [57] suggest using the epigastric branches as tethers to hold the AV loop in position -or as an alternative, nylon stitches from the wall of the AV loop vessel to the surrounding tissue. Dong et al [36] tried to reduce thrombosis by reducing the number of anastomoses. Instead of using the IVG, they anastomosed the popliteal artery with the femoral vein and therefore could generate an AV loop that was long enough even without the IVG.…”

Section: Challengesmentioning

confidence: 99%

“…Surgery time can be reduced and microsurgeons do not need a lot of experience. However, in many studies the vascularization by the AV loop was found to be superior compared to the AV bundle [35,36,60,61]. According to Wu et al [35] the vascular bundle model achieved a lower degree of vascularization but simultaneously a better balance between bone formation and scaffold absorption and a higher success rate.…”

Section: Challengesmentioning

confidence: 99%

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The Arteriovenous Loop: Engineering of Axially Vascularized Tissue

et al. 2018

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Background: Most of the current treatment options for large-scale tissue defects represent a serious burden for the patients, are often not satisfying, and can be associated with significant side effects. Although major achievements have already been made in the field of tissue engineering, the clinical translation in case of extensive tissue defects is only in its early stages. The main challenge and reason for the failure of most tissue engineering approaches is the missing vascularization within large-scale transplants. Summary: The arteriovenous (AV) loop model is an in vivo tissue engineering strategy for generating axially vascularized tissues using the own body as a bioreactor. A superficial artery and vein are anastomosed to create an AV loop. This AV loop is placed into an implantation chamber for prevascularization of the chamber inside, e.g., a scaffold, cells, and growth factors. Subsequently, the generated tissue can be transplanted with its vascular axis into the defect site and anastomosed to the local vasculature. Since the blood supply of the growing tissue is based on the AV loop, it will be immediately perfused with blood in the recipient site leading to optimal healing conditions even in the case of poorly vascularized defects. Using this tissue engineering approach, a multitude of different axially vascularized tissues could be generated, such as bone, skeletal or heart muscle, or lymphatic tissues. Upscaling from the small animal AV loop model into a preclinical large animal model could pave the way for the first successful attempt in clinical application. Key Messages: The AV loop model is a powerful tool for the generation of different axially vascularized replacement tissues. Due to minimal donor site morbidity and the possibility to generate patient-specific tissues variable in type and size, this in vivo tissue engineering approach can be considered as a promising alternative therapy to current treatment options of large-scale defects.

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Biomaterial‐Induction of a Transplantable Angiosome

Charbonnier

Maillard

Sayed

et al. 2019

Adv Funct Materials

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Creating transplantable vascular networks (angiosomes) that are fed and drained by vessels large enough to be surgically reconnected is key to harnessing the potential of regenerative medicine and advancing reconstructive surgical techniques. Currently, the only way to create a new angiosome is nontrivial and involves pressurizing a vein graft by its surgical attachment to an artery forming an arteriovenous loop (AVL). Material induction of a venous angiosome is reported, by placement of a 3D printed microporous monetite scaffold around a vein and its transplantability is further demonstrated. When the transplanted venosome is cut, it bleeds, illustrating potential reconstructive functionality. The volume of blood vessels generated by biomaterial-induction is as great as by AVL. Direct contact of the material with the vein does not appear to be critical to luminal sprouting, and wrapping the implant in a silicone membrane significantly reduces sprouting. Pilot studies with microporous polymeric scaffolds induce far less vascular invasion. After 4 weeks, monetite scaffolds are extensively vascularized and can be transplanted to an arterial vessel. This report is significant since a lack of tools to control vascular generation is an impediment to the treatment of several conditions that give rise to tissue ischemia and tissue reconstruction.

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Prefabrication of axial vascularized tissue engineering coral bone by an arteriovenous loop: A better model

Cited by 21 publications

References 26 publications

Effect of Xin Mai Jia on atherosclerosis in rats

Effect of Xin Mai Jia on atherosclerosis in rats

The Arteriovenous Loop: Engineering of Axially Vascularized Tissue

Biomaterial‐Induction of a Transplantable Angiosome

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