Preface

Colowick, Sidney P.; Kaplan, Nathan O.

doi:10.1016/0076-6879(55)01001-x

Cited by 92 publications

(99 citation statements)

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“…Reduced o-dianozidine was measured by spectrophotometer at 410 nm. 6 Determination of CAT CAT activity was measured according to the method that was defined by Beutler. 7 Principle CAT catalyses the breakdown of H 2 O 2 to H 2 O and O 2 .…”

Section: Determination Of Mpomentioning

confidence: 99%

Role of oxidative stress enzymes in open-angle glaucoma

Yıldırım

Ateş

Ercan

et al. 2004

Eye

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Purpose To investigate the role of oxidative stress and lipid peroxidation in the pathogenesis of primary open-angle glaucoma (POAG). Materials and methods The activities of myeloperoxidase (MPO), catalase (CAT), and the levels of plasma malondialdehyde (MDA) were measured in 40 (15 men and 25 women) patients with POAG and 60 (30 men and 30 women) healthy controls. Results There was no significant difference in the activities of CAT and MPO between the POAG patients and the controls. However, the plasma MDA level was significantly higher in patients than the controls. Conclusion The results of this preliminary study suggest that the possible alterations of plasma MDA levels may be associated with the pathogenesis of POAG, but further research is needed to understand the role of oxidative damage in this important disorder of aging.

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Section: Determination Of Mpomentioning

confidence: 99%

Role of oxidative stress enzymes in open-angle glaucoma

Yıldırım

Ateş

Ercan

et al. 2004

Eye

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“…The crude enzyme was precipitated by the addition of solid (NH 4 ) 2 SO 4 to 90% saturation according to Colowick and Kaplan [1955]. The sedimentary protein was collected by centrifugation at 5000 rpm for 15 min at 4°C.…”

Section: Purifi Cation Of Rosemary Tgasementioning

confidence: 99%

Isolation, purifi cation and characterisation of transglutaminase from rosemary (Rosmarinus officinalis L.) leaves

El-Hofi

Ismail

Nour

et al. 2014

Acta Sci Pol Technol Aliment

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Background. Rosemary (Rosmarinus offi cinalis L.) is a spice and medicinal herb widely used around the world of the natural antioxidants, and it has been widely accepted as one of the spices with the highest antioxidant activity. Transglutaminase (EC 2.3.2.13; TGase) is an enzyme capable of catalysing acyl transfer reactions by introducing covalent cross-links between proteins, as well as peptides and various primary amines. TGase activity in plants was fi rst observed in pea seedlings, and subsequently found in organs of both lower and higher plants. Recently, TGase has captured researchers' interest due to its attractive potential application in food industries. Therefore, the objectives of this study are isolation and purifi cation of TGase from new plant source rosemary (Rosmarinus offi cinalis L.) leaves at the laboratory scale. Moreover, investigation of the biochemical properties of the purifi ed TGase to provide a suitable TGase enzyme for food industry applications are in focus. Material and methods. Rosemary (Rosmarinus offi cinalis L.) leaves was used as a new plant source to TGase. The biochemical characteristics of the crude and purifi ed enzyme were determined. Results. Rosemary (Rosmarinus offi cinalis L.) TGase was purifi ed to homogeneity by successive three purifi cation steps including ammonium sulfate precipitatation, ion exchange chromatography on DEAE-Sephadex A-50 column and Size exclusion column chromatography on Sephadex G-100 column. Under experimental conditions, 20-30% of ammonium sulfate saturation in the enzyme solution had a high yield of enzyme activity could be obtained. The purifi ed enzyme from the Sephadex G-100 column had 21.35% yield with increased about 7.31 in purifi cation fold. Rosemary TGase exhibited optimum activity at pH 7.0 and 55°C for the catalytic reaction of hydroxylamine and Z-Gln-Gly. The purifi ed TGase almost maintained full activity after incubation for 15 min up to 60°C and it was completely inactivated at 85°C. The rosemary TGase was stimulated at 2-6 mM CaCl 2 concentrations while it lost about 5-20% from its activity by increasing CaCl 2 concentration. Sodium chloride (2-14%) shows no noticeable inhibition of the purifi ed TGase activity. Mg +2 , Ba +2 were acivited by the purifi ed TGase while it was strongly inhibited by Fe +2 , moderately by Cu +2 and Mn +2 . Conclusion. This paper reports on the purifi cation and characterisation of TGase from newly isolated plant, rosemary (Rosmarinus offi cinalis L.) leaves. Finding results of the TGase properties make this enzyme a good candidate for application in the food industry. However, additional work is required to increase activity yield during extraction and purifi cation for commercial scale of TGase from this plant.

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“…The values were 0.38 and 0.96 mg in El and EII, respectively. The total activity of the proteases was measured by recording tyrosine produced from casein according to Colowick and Kaplan (1955 Marquardt (1983). Amino-acid contents of feed and excreta were determined by ion-exchange chromatography using an autoanalyzer (Amino Acid Analyzer LC 5000, Biotronik), after 24 h acid hydrolysis with 6 M aqueous HCI at 115°C.…”

Section: Introductionmentioning

confidence: 99%

Effect of age and exogenous amylase and protease on development of the digestive tract, pancreatic enzyme activities and digestibility of nutrients in young meat-type chicks

Mahagna¹,

Nir²,

Larbier³

et al. 1995

Reprod. Nutr. Dev.

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Summary― Day-old male meat-type chicks were fed a commercial starter diet supplemented with 2 levels of enzyme preparations containing amylase and proteases up to 14 d of age. Enzyme supplementation had no significant effect on feed intake or growth rate, and was accompagnied by a significant decrease in gizzard content and small intestine weight. The intestine contents increased and this increase was accompanied by a significant decrease in its pH. Enzyme supplementation depressed the activity of chymotrypsin in the pancreas and the'activity of amylase, trypsin and chymotrypsin in the intestinal contents. Some carry-over effects were observed on d 42, 4 weeks after the cessation of the enzyme supplements. These were mainly a significant depression in the activity of trypsin in the intestinal contents. In a balance study, diets supplemented with 0, 250 and 1 000 pg/kg enzyme preparations were supplied. Exogenous enzyme supplements had no significant effect on the digestibility of all the nutrients studied except for the highest level of enzyme supplementation, which improved slightly but consistently the digestibility of amino acids. Some age effects were observed, mainly a decrease in the digestibility of fat and starch, and in the ME of the diet from weeks 1 to 2 followed by an increase during week 3. Protein digestibility and retention of nitrogen decreased with age.

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Preface

Cited by 92 publications

References 0 publications

Role of oxidative stress enzymes in open-angle glaucoma

Role of oxidative stress enzymes in open-angle glaucoma

Isolation, purifi cation and characterisation of transglutaminase from rosemary (Rosmarinus officinalis L.) leaves

Effect of age and exogenous amylase and protease on development of the digestive tract, pancreatic enzyme activities and digestibility of nutrients in young meat-type chicks

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