Preferential enhancement of myoblast differentiation by insulin‐like growth factors (IGF I and IGF II) in primary cultures of chicken embryonic cells

Schmid, Christoph; Steiner, Th.; Froesch, E. R.

doi:10.1016/0014-5793(83)80742-x

Cited by 159 publications

(49 citation statements)

References 9 publications

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“…Because of the cross-reactivities to their receptors, these studies do not demonstrate whether insulin is acting on the CFU-E through its own receptors or through IGF-I receptors (33,34). It has been accepted that the effects of IGF-I may be initiated by insulin, but a concentration of insulin of about 20-100 times greater than that of IGF-I is necessary in most systems (20,21,(35)(36)(37). This would indicate that large concentrations of insulin may be acting on the CFU-E through interaction with IGF-I receptors.…”

Section: Resultsmentioning

confidence: 99%

Human colony-forming units-erythroid do not require accessory cells, but do require direct interaction with insulin-like growth factor I and/or insulin for erythroid development.

Sawada¹,

Krantz²,

Dessypris³

et al. 1989

J. Clin. Invest.

189

108

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The presence of heterogeneous erythroid progenitor cells, contaminant cells, or serum may alter erythroid colony development in vitro. To obtain highly purified colony-forming unitserythroid (CFU-E), we cultured partially purified human blood burst-forming units-erythroid (BFU-E) in methylcellulose with recombinant human erythropoietin (rHuEPO) for 7 d and generated cells that consisted of 30-60% CFU-E, but no BFU-E. A serum-free medium was used that allowed development of the same number of erythroid colonies as serum containing medium, but with a greater percentage of larger colonies. This medium consisted of delipidated crystalline bovine serum albumin, iron saturated transferrin, lipid suspension, fibrinogen, thrombin, Iscove's modified Dulbecco's medium/F-12IHAMI, and insulin plus rHuEPO. When CFU-E were cultured in a limiting dilution assay and the percentage of nonresponder wells was plotted against cell concentration, both serum-free cultures and serum-containing cultures yielded overlapping straight lines through the origin indicating that CFU-E development did not depend on accessory cells and that insulin acted directly on the CFU-E. Human recombinant interleukin 3 (IL-3) and/or granulocyte-macrophage colony-stimulating factor had no effect on CFU-E growth, while they markedly enhanced BFU-E growth. Physiological concentrations of recombinant human insulin-like growth factor I (IGF-I) enhanced CFU-E growth in the absence of insulin and, together with rHuEPO in serum-free medium, provided a plating efficiency equal to that of serum-containing medium. Limiting dilution analysis in serum-free medium with IGF-I showed a straight line through the origin indicating that IGF-I also acted directly on the CFU-E and not through an effect on accessory cells. These data demonstrate that CFU-E do not require accessory cells, but do require IGF-I and/or insulin which act directly on the CFU-E.

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Section: Resultsmentioning

confidence: 99%

Human colony-forming units-erythroid do not require accessory cells, but do require direct interaction with insulin-like growth factor I and/or insulin for erythroid development.

Sawada¹,

Krantz²,

Dessypris³

et al. 1989

J. Clin. Invest.

189

108

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“…An in vitro study, using avian (turkey) satellite cells, has shown that IGF-2 mRNA was expressed at higher levels during the proliferative phase, followed by a marginal reduction during differentiation (Ernst et al, 1996). Like IGF-1, IGF-2 is also a potent regulator of chicken muscle cell proliferation (Duclos et al, 1991) and differentiation (Schmid et al, 1983). However, unlike IGF-1, IGF-2 administration does not promote lean growth in young chicken (Tomas et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Neuromuscular stimulation causes muscle phenotype‐dependent changes in the expression of the IGFs and their binding proteins in developing slow and fast muscle of chick embryos

McEntee

Simbi

Bayol

et al. 2006

Developmental Dynamics

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Insulin-like growth factor (IGF) -1 and -2 and binding protein (IGFBP-2, -4, and -5) expression can be affected by several environmental factors, but the impact of movement on the IGF axis during late embryogenesis has yet to be fully characterized. Movement was promoted in chick embryos during midembryogenesis using 4-aminopyridine (4-AP). The results indicate an increase in IGF-1 (P < 0.01) and a decrease in IGFBP-2 (P < 0.05) mRNA expression in slow muscle of the stimulated group compared with the control group. In fast muscle, there was a decrease in IGF-2 (P < 0.01) on embryonic day (ED) 16 and an increase in IGFBP-2 (P < 0.01) and IGFBP-4 (P < 0.05) and in IGFBP-5 (P < 0.05) expression on ED18 in the stimulated group compared with the control group. These results indicate that neuromuscular stimulation with 4-AP influences IGF axis gene expression in a muscle fiber type-dependent manner. Consequences of the changes in the IGF system for each muscle are discussed.

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“…In vitro, it stimulates the proliferation of a wide variety of cultured cells that are primarily of mesodermal origin (3)(4)(5). In addition, it can cause some cells to differentiate including chondrocytes (6), myoblasts (7), osteoblasts (8) and erythroid precursors (9). In vivo, IGF-I regulates postnatal skeletal growth (5,10).…”

Section: Introductionmentioning

confidence: 99%

Sequences of liver cDNAs encoding two different mouse insulin-like growth factor I precursors

Bell¹,

Stempien²,

Fong³

et al. 1986

Nucl Acids Res

214

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Complementary DNAs encoding mouse liver insulin-like growth factor I (ICF-I) have been isolated and sequenced. Alternative RNA splicing results in the synthesis of two types of mouse IGF-I precursor that differ in the size and sequence of the COOH-terminal peptide.The sequences of the signal peptides, IGF-I moieties and the first 16 amino acids of the COOH-terminal peptides or E-domains of the two precursors are identical.The sequence difference results from the presence in preproIGF-IB mRNA of a 52 base insertion which introduces a 17 amino acid segment into the COOH-terminal peptide of preproIGF-IB and also causes a shift in the reading frame of the mRNA. As a consequence of this insertion, the COOH-terminal 19 and 25 amino acids of mouse preproIGF-IA and -IB, respectively, are different. The sequences of mouse and human preproIGF-IA are highly conserved and possess 94% identity.In contrast, the sequences of mouse and human preproIGF-IB are quite different in the region of the COOH-terminal peptide. A comparison of the sequences of mouse and human preproIGF-IB mRNA indicates that they are generated by different molecular mechanisms.

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Preferential enhancement of myoblast differentiation by insulin‐like growth factors (IGF I and IGF II) in primary cultures of chicken embryonic cells

Cited by 159 publications

References 9 publications

Human colony-forming units-erythroid do not require accessory cells, but do require direct interaction with insulin-like growth factor I and/or insulin for erythroid development.

Human colony-forming units-erythroid do not require accessory cells, but do require direct interaction with insulin-like growth factor I and/or insulin for erythroid development.

Neuromuscular stimulation causes muscle phenotype‐dependent changes in the expression of the IGFs and their binding proteins in developing slow and fast muscle of chick embryos

Sequences of liver cDNAs encoding two different mouse insulin-like growth factor I precursors

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