Preferential expansion of human umbilical cord blood-derived CD34-positive cells on major histocompatibility complex-matched amnion-derived mesenchymal stem cells

Abstract

BackgroundWe previously found in a murine hematopoietic system that hematopoietic stem cells show high differentiation and proliferation capacity on bone marrow-derived mesenchymal stem cells/stromal cells (microenvironment) with "self" major histocompatibility complex (MHC). Design and MethodsWe examined whether amnion-derived adherent cells have the characteristics of mesenchymal stem cells, and whether these adherent cells can support the proliferation of umbilical cord blood-derived lineage-negative and CD34-positive cells (Lin -CD34 + cells) obtained from the same fetus to a greater extent than those derived from other fetuses. Results Culture CD34+ cells were cultured on the autologous (MHC-matched) or MHC-mismatched amnion-derived adherent cells in short-term assays (hematopoietic stem cell-proliferation) and long-term culture-initiating cell assays, greater expansion of the Lin -CD34+ cells was observed in the MHC-matched combination than in MHC-mismatched combinations. The concentration of granulocyte-macrophage colony-stimulating factor in the culture supernatants of the long-term culture-initiating cell assays was significantly higher in the MHC-matched combination than in MHC-mismatched combinations. ConclusionsIt is likely that a MHC restriction exists between hematopoietic stem cells and mesenchymal stem cells/stromal cells in the human hematopoietic system and that granulocutemacropage colony-stimulating factor contributes to some extent to the preferential hematopoiesis-supporting ability of the MHC-matched amnion-derived adherent cells.Key words: mesenchymal stem cells, CD34-positive cells, umbilical cord blood, amnion, major histocompatibility complex. Haematologica 2009; 94:618-628. doi:10.3324/haematol.2008 This is an open-access paper. Citation: Mizokami T, Hisha H, Okazaki S, Takaki T, Wang X-L, Song, C-Y, Li Q, Kato J, Hosaka N, Inaba M, Kanzaki H, and Ikehara S. Preferential expansion of human umbilical cord blood-derived CD34-positive cells on major histocompatibility complex-matched amnion-derived mesenchymal stem cells. ABSTRACTPreferential expansion of human umbilical cord blood-derived CD34-positive cells on major histocompatibility complex-matched amnion-derived mesenchymal stem cells

Mesenchymal Stem Cells Secreting Angiopoietin-Like-5 Support Efficient Expansion of Human Hematopoietic Stem Cells Without Compromising Their Repopulating Potential

“…MSCs from bone marrow [6], Wharton's jelly of umbilical cord segments [7], cord blood [8], and other fetal tissues [9][10][11] have been shown to support expansion of human HSCs [6][7][8][11][12][13][14][15][16]. Depending on the coculture conditions, 80-800-fold increase in total cell number and 4-100-fold increase in CD34 þ cells were reported [17][18][19].…”

Tanshinone IIA activates calcium-dependent apoptosis signaling pathway in human hepatoma cells

“…All the cells, treated or untreated, were positive for CD29, CD73, CD44, and CD166, but negative for CD34 and CD45. These phenotypes were similar to those of mesenchymal stem cells (MSC) derived from adult bone marrow and umbilical cord blood and other fetal tissues [16,17].…”

Perinatal sources of mesenchymal stem cells: Wharton’s jelly, amnion and chorion

“…The AM-MSCs retained a stable morphology for more than 20 passages. The phenotype of AM-MSCs (CD29, CD73, CD44 positive and CD14, CD34, CD45 negative) is similar to MSCs derived from adult bone marrow and umbilical cord blood [8]. The genetic profiles of the AM-MSCs were compared with the umbilical cord blood and bone marrowderived mesenchymal stem cells (BM-MSCs).…”

“…Due to the easy access, low immunogenicity, extensive proliferation potential, multipotency, cryopreservation, and minimal ethical concerns associated with stem cells from umbilical cord blood, intensive studies on the presence of stem cells in other perinatal tissues have been started. Multipotent cells have been found in the chorion, placenta, perivascular areas, amnion, amniotic fluid, and in the tissue surrounding the umbilical cord vessels, i.e., Wharton's jelly [7][8][9]. In this review paper we focus on the biology and therapeutic potential of mesenchymal stem cells derived from the umbilical cord, Wharton's jelly and two fetal membranes: the amnion and the chorion.…”