Preferential formation of 13-hydroxylinoleic acid by human peripheral blood eosinophils

Comparison of eight 15-lipoxygenase (LO) inhibitors on the biosynthesis of 15-LO metabolites by human neutrophils and eosinophils

“…While eosinophils are known to abundantly express 15-LO-1 and to synthesize 15-LO metabolites [ 2 , 12 , 15 , 24 , 25 ] the data regarding 15-LO-1 and -2 expression by neutrophils is conflicting and might reflect, at least in part, the presence of eosinophils in neutrophil suspensions, as supported with the depletion of eosinophils from neutrophil suspensions [ 16 ]. In this regard, we analyzed the expression of both 15-LO-1 and 15-LO-2 in eosinophil-depleted neutrophils and eosinophils.…”

Peroxisome Proliferator-Activated Receptor .GAMMA. Ligands Isolated from Adlay Seed (Coix lacryma-jobi L. var. ma-yuen STAPF.)

“…Both 9-HODE and 13-HODE are produced by cyclooxygenase and/or lipoxygenase in mammalian cells such as endothelial, epithelial, and smooth muscle cells, as well as blood cells including neutrophils, basophils, monocytes, and lymphocytes. [23][24][25][26] It has been reported that PPARg is acti- (2) (A) pPPREx3-TK-luc and pCMX-bgal were transfected into HEK293 cells together with pCMX-PPARg1. 20 h after the transfection, the cells were treated with EPA; eicosapentaenoic acid, LA; linoleic acid, OA; oleic acid, SA; stearic acid, 13-HODE, 9-HODE, 13-E,E-HODE (1) and 9-E,E-HODE (2) at the concentration of 40 mM for 20 h. Troglitazone (TGZ) was used at 5 mM.…”

“…29,30) Given that 9-HODE can be produced in monocytes or lymphocytes, 26) leading to activation of PPARg that is more potent than 13-HODE activation, 18) 9-HODE is expected to exhibit anti-inflammatory or immunomodulating activity more efficiently than 13-HODE. Therefore, 9-E,E-HODE (2), as a potent PPARg agonist, may also serve as an inflammatory modulator like 9-HODE.…”

Chemotaxis and activation of human peripheral blood eosinophils induced by pollen-associated lipid mediators

“…Linoleic as well as alinolenic acid structurally resemble arachidonic acid and can, as arachidonic acid, easily be converted to hydroxylated derivates either enzymatically or via auto-oxidation. 5,6 Recently, oxidized derivates of linoleic acid have been shown to exhibit a number of biological functions such as modulation of cell proliferation, apoptosis, and inflammation. [7][8][9] They have also been shown to be potent chemoattractants and activators of human polymorphonuclear granulocytes.…”