Preferential solvation of lysozyme by dimethyl sulfoxide in binary solutions of water and dimethyl sulfoxide

Kamiyama, Tadashi; Liu, H. L.; Kimura, Takayoshi

doi:10.1007/s10973-008-9232-7

Cited by 25 publications

(33 citation statements)

References 24 publications

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“…Compared to glycerol, little is known about the DMSO-lysozyme interactions, apart from a preferential hydration of lysozyme in water-DMSO mixtures indicating a weak or no binding of DMSO to lysozyme. 3,4 This suggests that DMSO mainly influences the dielectric properties of the solution, and is consistent with the observed weaker temperature dependence of the cut-off length δ(T) in the case of DMSO.…”

Section: A Refractive Indices and Static Dielectric Constantssupporting

confidence: 84%

“…26 Furthermore, Arakawa et al 3 and Kamiyama et al 4 observed a preferential hydration of lysozyme in water-DMSO mixtures, indicating that DMSO does not bind to the lysozyme surface. 4 This suggests that DMSO mainly affects the dielectric properties of the bulk solution. Nevertheless, DMSO leads to denaturation at volume fractions beyond 70 vol.%.…”

Section: Introductionmentioning

confidence: 99%

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Effect of glycerol and dimethyl sulfoxide on the phase behavior of lysozyme: Theory and experiments

Gögelein

Wagner

Cardinaux

et al. 2012

The Journal of Chemical Physics

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Salt, glycerol, and dimethyl sulfoxide (DMSO) are used to modify the properties of protein solutions. We experimentally determined the effect of these additives on the phase behavior of lysozyme solutions. Upon the addition of glycerol and DMSO, the fluid-solid transition and the gas-liquid coexistence curve (binodal) shift to lower temperatures and the gap between them increases. The experimentally observed trends are consistent with our theoretical predictions based on the thermodynamic perturbation theory and the Derjaguin-Landau-Verwey-Overbeek model for the lysozyme-lysozyme pair interactions. The values of the parameters describing the interactions, namely the refractive indices, dielectric constants, Hamaker constant and cut-off length, are extracted from literature or are experimentally determined by independent experiments, including static light scattering, to determine the second virial coefficient. We observe that both, glycerol and DMSO, render the potential more repulsive, while sodium chloride reduces the repulsion.

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Section: A Refractive Indices and Static Dielectric Constantssupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Effect of glycerol and dimethyl sulfoxide on the phase behavior of lysozyme: Theory and experiments

Gögelein

Wagner

Cardinaux

et al. 2012

The Journal of Chemical Physics

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“…10,12,13 As expected, the methyl groups face hydrophobic surfaces leaving the sulfoxide available for hydrogen bonding. We used a variety of spectroscopic and calorimetric techniques to test the hypothesis that cosolvents directly interact with amino-acid residues important in network formation and subsequently decrease solution viscosity.…”

Section: Polar Solvents Preferentially Solvate and Directly Interact supporting

confidence: 66%

“…Indeed, polar solvents have been shown to preferentially solvate hydrophobic surfaces and interact with protein residues through hydrogen bonding and dispersive-type interactions. [10][11][12][13] Large-length-scale water density fluctuations near hydrophobic surfaces have been shown to control hydrophobic assembly in aqueous solution. 14 If protein self-association is at least partially mediated via dispersive-type interactions, then at high concentrations as the two surfaces (protein-protein) approach each other, formation of the soft liquid-vapor-like interface characteristic of the hydrophobic force of assembly could be induced.…”

Section: Introductionmentioning

confidence: 99%

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Polar Solvents Decrease the Viscosity of High Concentration IgG1 Solutions Through Hydrophobic Solvation and Interaction: Formulation and Biocompatibility Considerations

Kamerzell

Pace

et al. 2013

Journal of Pharmaceutical Sciences

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Toxic effects of dimethyl sulfoxide on red blood cells, platelets, and vascular endothelial cells in vitro

Yi¹,

Min-xia²,

Luo

et al. 2017

FEBS Open Bio

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Dimethyl sulfoxide (DMSO) is widely used in biological studies as a cryoprotective agent for cells and tissues, and also for cryopreserved platelets (PLTs). However, few data on the toxic effects of DMSO following intravenous infusion of cryopreserved PLTs are available. The aim of this study was to explore dose‐related effects of DMSO on red blood cells (RBCs), PLTs and vascular endothelial cells in vitro. The results showed that DMSO treatments had significant effects on RBCs, affecting osmotic fragility and increasing hemolysis. Free hemoglobin (FHb) level of RBCs was 0.64 ± 0.19 g L−1 after incubation for 6 h with 0.6% DMSO, and these levels were elevated compared with controls (0.09 ± 0.05 g L−1). Aggregation of PLTs induced by adenosine diphosphate, thrombin (THR), and thrombin receptor activator peptide (TRAP) were inhibited by DMSO treatment because the THR generation capacity was reduced. The intensity of the cytosolic esterase‐induced fluorescence response from carboxy dimethyl fluorescein diacetate (CMFDA) in PLTs was decreased about 29% ± 0.04% after treatment with DMSO. DMSO also inhibited the proliferation of the vascular endothelial cell line EAhy926 cells by blocking the G1 phase. Apoptosis of EAhy926 cells with 0.6% DMSO stimulation was increased threefold compared to controls. On the basis of these findings, it was concluded that DMSO was toxic to the hematologic system. This should be taken into account when assessing the infusion effects of cryopreserved PLTs or other blood products requiring DMSO as a vehicle, such as cryopreserved stem cells, in order to avoid adverse therapeutic effects.

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Preferential solvation of lysozyme by dimethyl sulfoxide in binary solutions of water and dimethyl sulfoxide

Cited by 25 publications

References 24 publications

Effect of glycerol and dimethyl sulfoxide on the phase behavior of lysozyme: Theory and experiments

Effect of glycerol and dimethyl sulfoxide on the phase behavior of lysozyme: Theory and experiments

Polar Solvents Decrease the Viscosity of High Concentration IgG1 Solutions Through Hydrophobic Solvation and Interaction: Formulation and Biocompatibility Considerations

Toxic effects of dimethyl sulfoxide on red blood cells, platelets, and vascular endothelial cells in vitro

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