Preferential targeting of conserved Gag regions after vaccination with a heterologous DNA prime Modified Vaccinia Ankara boost HIV vaccine regime

Bauer, Andreas; Podola, Lilli; Haule, Antelmo; Sudi, Lwitiho; Nilsson, Charlotta; Mann, Philipp; Missanga, Marco; Kaluwa, B.; Maboko, Leonard; Lueer, Cornelia; Mwakatima, Maria; Aboud, Said; Bakari, Muhammad; Currier, Jeffrey R.; Robb, ML; Joseph, Sarah; McCormack, Sheena; Lyamuya, E. F.; Wahrén, Britta; Sandström, Eric; Biberfeld, Gunnel; Höelscher, Michael; Kroidl, Arne; Geldmacher, Christof

doi:10.1186/1742-4690-9-s2-p330

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“…Another challenge is genetic diversity of virus that resulted in immune escape, alwaysrecombinant vaccines design prefers to target conserved antigenic regions of virus, it might accelerates better immune response 20 . The KFDV vaccinated individuals were reported for disease occurrence, it may because of variations in circulating virus 21 .…”

Section: Discussionmentioning

confidence: 99%

In-Silico Design of Envelope based Multi-epitope Vaccine Candidate Against Kyasanur Forest Disease Virus

Arumugam

2021

Preprint

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Kyasanur Forest Disease Virus (KFDV) causing common tick-borne hemorrhagic fever in south India, there is no approved anti-viral or efficacious vaccine against this disease. Recent KFDV spread into new geographic locations gives urgent call for development of new vaccine and drugs. In this study, we adapted in-silico approach to design multi-epitope subunit vaccine for KFDV. Conserved regions of KFDV envelope protein sequences reported during 1962 to 2016 were identified. Eight different immuno-informatics tools were employed to predict the linear B-cell and T-cell epitopes, high scored and/or multi-immunogenic epitopes were linked together and obtained two vaccine candidates (VC1 and VC2). Obtained vaccine candidates were found to be non-allergic and had good antigenic properties, also gives the cross-protection against to Alkhurma Hemorrhagic Fever virus (AHFV). The 3D structures of vaccine candidates were built and validated. Docking of vaccine candidates with toll-like receptor-8 (TLR-8) was performed by Hex 8.0 and Cluspro, highest binding energy observed between VC2 and TLR8. JCAT sever confirmed cloning efficiency of both vaccine constructs and in-silico cloning into pET30a (+) vector by SnapGene suggests successful translation of vaccine constructs. In this study, multi-epitope vaccine candidates were designed and validated, it paves the way for up-coming vaccine and diagnostic kit development.

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Section: Discussionmentioning

confidence: 99%

In-Silico Design of Envelope based Multi-epitope Vaccine Candidate Against Kyasanur Forest Disease Virus

Arumugam

2021

Preprint

View full text Add to dashboard Cite

show abstract

Preferential targeting of conserved Gag regions after vaccination with a heterologous DNA prime Modified Vaccinia Ankara boost HIV vaccine regime

Cited by 1 publication

References 0 publications

In-Silico Design of Envelope based Multi-epitope Vaccine Candidate Against Kyasanur Forest Disease Virus

In-Silico Design of Envelope based Multi-epitope Vaccine Candidate Against Kyasanur Forest Disease Virus

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