Prefermented cereals containing fungal gamma-linolenic acid and their effect on rumen metabolism in vitro

Abstract

ABSTRACT:The application of Thamnidium elegans fungal strain CCF 1456 (TE) for effective utilization of various agroindustrial materials creates new perspectives for animal cereal diets enriched with microbial γ-linolenic acid (GLA). Diets consisting of lucerne hay (LH) plus prefermented cereals (wheat bran/spent malt grains, WB+TE or WB+TE enriched with sunflower oil, WB+SO+TE in the first experiment and ground maize grains, GC+TE in the second experiment) were used in the artificial rumen. We examined their effect on the rumen fermentation pattern and lipid metabolism. The diet affected the results of degradability of dry matter, organic matter, neutral detergent fibre and acid detergent fibre of LH+WB diets (P < 0.05 and P < 0.01). The GLA daily output of prefermented diet substrates LH+WB+TE and LH+WB+SO+TE, or LH+GC+TE was higher compared to the non-prefermented LH+WB or LH+GC, respectively (P < 0.05 and P < 0.01). Daily outputs of trans11 oleic (TVA) of the LH+GC+TE diet were higher versus the non-prefermented LH+GC (P < 0.01). The biohydrogenation of fatty acids (C18:1 cis9 oleic, C18:2 linoleic, C18:3n-3 alpha-linolenic, C18:3n-6 GLA and total FA) of prefermented cereal diets was not influenced. Cereal diets containing microbial GLA might positively enhance GLA daily outputs in the RUSITEC effluent, but they are not effective enough to decrease the biohydrogenation of unsaturated fatty acids.

Effect of feeding of 5% prefermented cereal-based bioproduct enriched with γ-linolenic acid on production indicators, chemical composition, fatty acid profile and lipid oxidation of broiler meat

“…Modifications in the fatty acid composition of poultry meat require addition of the PUFA source to feed. A current global trend in the production of diets with supplemented components of PUFA has increased the demand for feeds containing GLA in animal nutrition (Laho et al 2011). However, GLA is rarely found in standard feed.…”

Effect of adding prefermented cereal product containing gamma-linolenic acid to broiler feed on production indicators and fatty acid profile of chicken breast

“…Poultry production is a potential alternative and sustainable source of PUFA. The current worldwide trend in the production of diets with supplemented components of PUFA has increased the demand for feeds containing γ-linolenic acid (GLA) for animal nutrition (Laho et al 2011). Gamma-linolenic acid (C18:3 n-6) via conversion to prostaglandin E1 shows antiinflammatory, antithrombotic, antiproliferative, and lipid-lowering potential (Kawashima et al 2009).…”

Substrates enriched by the fungusCunninghamella echinulata: anin vitrostudy of nutrient composition, sheep rumen fermentation and lipid metabolism

“…Our findings are consistent with prior results, with the fungus Thamnidium elegans fermented in rumen fluid (Laho et al . ,b). On the other hand, the control substrates (i.e., WB, CM and BF) and the substrates with brewer's grains (i.e., WBG and CMG) had similar DM, with a mean of DM 909 g kg −1 .…”

“…However, only limited research related to application of oleaginous micro‐organisms for preparation of PUFA‐enriched feed for ruminant nutrition is available (Laho et al . ,b). The objectives of this study were to determine the effects of C. echinulata ‐enriched substrates (i) on rumen fermentation processes and (ii) on rumen lipid metabolism.…”