Preformed gelatin microcryogels as injectable cell carriers for enhanced skin wound healing

Zeng, Yang; Zhu, Lin; Han, Qin; Liu, Wei; Mao, Xiaojing; Li, Yaqian; Yu, Nanze; Feng, Siyu; Fu, Qinyouen; Wang, Xiaojun; Du, Yanan; Zhao, Robert Chunhua

doi:10.1016/j.actbio.2015.07.042

Cited by 108 publications

(64 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Wound healing has also been a targeted research area for potential stem cell-derived therapies. Delivery of hASCs for full thickness skin wounds using injectable gelatin microspheres was shown to significantly improve the wound healing rate, stem cell retention, and growth factor secretion levels compared to delivery of cells alone [170]. Similar functional results in wound healing have been observed when BMSCs were delivered using cell-protective alginate beads within injectable hydrophobic poly(ether urethane) hydrogels compared to implanting pre-formed scaffolds [171] or transplanting cells within gelatin-poly(ethylene glycol) hydrogels [172].…”

Section: Specific Hydrogel Design Choices For Specific Tissue Applmentioning

confidence: 99%

Design of Injectable Materials to Improve Stem Cell Transplantation

2016

View full text Add to dashboard Cite

Stem cell-based therapies are steadily gaining traction for regenerative medicine approaches to treating disease and injury throughout the body. While a significant body of work has shown success in preclinical studies, results often fail to translate in clinical settings. One potential cause is the massive transplanted cell death that occurs post injection, preventing functional integration with host tissue. Therefore, current research is focusing on developing injectable hydrogel materials to protect cells during delivery and to stimulate endogenous regeneration through interactions of transplanted cells and host tissue. This review explores the design of targeted injectable hydrogel systems for improving the therapeutic potential of stem cells across a variety of tissue engineering applications with a focus on hydrogel materials that have progressed to the stage of preclinical testing.

show abstract

Section: Specific Hydrogel Design Choices For Specific Tissue Applmentioning

confidence: 99%

Design of Injectable Materials to Improve Stem Cell Transplantation

2016

View full text Add to dashboard Cite

show abstract

“…First, it is easier to achieve high cell density in the modular tissues (usually less than 1 mm in length) than in bulk tissues (BTs, usually more than 1 mm in length), which makes it possible to fabricate complicated 3D grafts with a random size and uniform distribution of seed cells. 16 Second, the BU approach is more suitable for biomimetic applications as many tissues are organized into repeating functional units, such as the osteon in bone and the myofiber in muscle. 17 However, it is still unknown whether the in vivo osteogenic performance of TEBGs fabricated by BU strategy are superior to those fabricated using the TD strategy.…”

Section: Introductionmentioning

confidence: 99%

An in vivo comparative study of the gelatin microtissue‐based bottom‐up strategy and top‐down strategy in bone tissue engineering application

Luo

Fang

et al. 2018

J Biomedical Materials Res

View full text Add to dashboard Cite

Tissue‐engineered bone grafts (TEBGs) represent a promising treatment for bone defects. Nevertheless, drawbacks of the current construction strategy (top‐down [TD] strategy) such as limited transmission of nutrients and nonuniform distribution of seeded cells, result in an unsatisfied therapeutic effect on large segmental bone defects. Theoretically, tissue‐engineered microtissue (TEMT)‐based bottom‐up (BU) strategy is effective in preserving seed cells and vascularization, thus being regarded as a better alternative for TEBGs. Yet, there are few studies focusing on the comparison of the in vivo performance of TEBGs fabricated by TD or BU strategy. Here, we developed an ectopic bone formation rat model to compare the performance of these two construction strategies in vivo. TEBGs made from gelatin TEMT (BU strategy) and bulk tissue (BT; TD strategy) were seeded with equal number of rat bone marrow‐derived mesenchymal stem cells and fabricated in 5 mm polydimethylsiloxane chambers. The grafts were implanted into subcutaneous pockets in the same rat. Four weeks after implantation, microcomputed tomography and hematoxylin and eosin staining results demonstrated that more bony tissue was formed in the microtissue (MT) group than in the BT group. CD31 staining further confirmed that there were more blood vessels in the MT group, indicating that the BU strategy was superior in inducing angiogenesis. This comparative study provides evidence that the BU construction strategy is more effective for in vivo application and bone defect treatment by bone tissue engineering. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 678–688, 2019.

show abstract

“…Beside, artificial dermis consisted of type I collagen fiber coated with 3% a-elastin hydrolysate reduced human burn wound contracture and promoted dermal reconstruction [69]. In most recent reports, MSCs were encapsulated in and delivered by gelatin microsphere and gelatin microcryogels to the margins of dermal wound and were found to accelerate wound closure rate and prevent scarring by maintaining MSC-released protein [70, 71]. Li et al demonstrated that 3-dimentional graphene foam (3D-GF) loaded with MSCs decreases scar formation, potentially because of biomechanical and biochemical signals from 3D-GFs.…”

Section: Introductionmentioning

confidence: 99%

Biomaterials and tissue engineering for scar management in wound care

2017

View full text Add to dashboard Cite

Scars are a natural and unavoidable result from most wound repair procedures and the body’s physiological healing response. However, they scars can cause considerable functional impairment and emotional and social distress. There are different forms of treatments that have been adopted to manage or eliminate scar formation. This review covers the latest research in the past decade on using either natural agents or synthetic biomaterials in treatments for scar reduction.

show abstract

Preformed gelatin microcryogels as injectable cell carriers for enhanced skin wound healing

Cited by 108 publications

References 63 publications

Design of Injectable Materials to Improve Stem Cell Transplantation

Design of Injectable Materials to Improve Stem Cell Transplantation

An in vivo comparative study of the gelatin microtissue‐based bottom‐up strategy and top‐down strategy in bone tissue engineering application

Biomaterials and tissue engineering for scar management in wound care

Contact Info

Product

Resources

About