Preformulation Studies and Physicochemical Properties of Intranasal Low Sialic Acid Erythropoietin

Muñoz-Cernada, A.; Cardentey-Fernández, J.; Fernández, L.; Calvo, Loany; Figueredo-Gonzáles, T.; Rodríguez-Abreu, Delvys; Fernández-Cervera, Mirna; Sosa-Testé, I.; Amaro-González, Daniel

doi:10.12665/j152.amaro

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Preparation Of Physical Mixtures/binary Blends1

Non-denaturing Sds-page and Densitometry1

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2019

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“…One set of tubes were analyzed as soon as prepared. The second sets of samples were assigned as control samples and stored at 5±3°C [13,14]. These samples were evaluated further by FTIR, non-denaturing SDS-PAGE while third sets of samples were stored for isothermal stress studies (IST) at 25°C ± 2°C/60 ± 5% RH in dark stability chamber as per ICH guidelines.…”

Section: Preparation Of Physical Mixtures/binary Blendsmentioning

confidence: 99%

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Analytical Approaches in Unveiling the Compatibility of Collagenase With Additives in View of Its Colloidal Delivery

Vijay

Krishnamoorthy

2019

J. Chil. Chem. Soc.

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The core objective of present work was to investigate the chemical interactions of selected additives with therapeutic enzyme Collagenase (COLG) and determination of its compatibility for futuristic colloidal formulation. Initially, solubility of COLG with additives was determined and emulsification efficiency of surfactants was examined. FTIR spectra of COLG with its binary blends were compared. Non-denaturing SDS-PAGE and Circular Dichroism (CD) used to investigate primary and secondary structure of enzyme in binary mixture. Finally, the control and accelerated studies was performed by spectroscopy.

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Section: Preparation Of Physical Mixtures/binary Blendsmentioning

confidence: 99%

“…The destained gels were imaged and analyzed using GelQuant software (BiochemLab Solution). The band volumes (pixel intensity) of the peaks corresponding to the COLG standards concentrations were recorded and the amount was calculated from the regression equation obtained from the calibration plot [14,[19][20].…”

Section: Non-denaturing Sds-page and Densitometrymentioning

confidence: 99%

Analytical Approaches in Unveiling the Compatibility of Collagenase With Additives in View of Its Colloidal Delivery

Vijay

Krishnamoorthy

2019

J. Chil. Chem. Soc.

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show abstract

Preformulation Studies and Physicochemical Properties of Intranasal Low Sialic Acid Erythropoietin

Cited by 1 publication

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Analytical Approaches in Unveiling the Compatibility of Collagenase With Additives in View of Its Colloidal Delivery

Analytical Approaches in Unveiling the Compatibility of Collagenase With Additives in View of Its Colloidal Delivery

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