Pregabalin Alters Nociceptive Behavior and Expression Level of P2X3 Receptor in the Spinal Dorsal Horn in a Rat Model Induced by Chronic Compression of the Dorsal Root Ganglion

Yu, Jianfeng; Fu, Peng; Zhang, Yan; Liu, Shuzhen; Cui, Donghong

doi:10.1002/ar.22816

Cited by 11 publications

(5 citation statements)

References 34 publications

(37 reference statements)

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“…P2X3 receptor activation is closely correlated with inflammatory pain ( 40 ). It has been reported that the downregulation of P2X3 receptor expression may be involved in acupuncture analgesia in the spinal cord of rats with chronic constriction injuries ( 41 ), suggesting that the P2X3 receptor serves an essential role in the regulation of peripheral and central nervous system pain.…”

Section: Discussionmentioning

confidence: 99%

Methylene blue relieves the development of osteoarthritis by upregulating lncRNA MEG3

et al. 2018

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Methylene blue (MB) is a long-term inhibitor of peripheral nerve axons, thereby alleviating or permanently eliminating pain. However, it remains unknown whether MB is safe and effective method of treating osteoarthritis (OA). MB was injected into the knee joints of rabbits and they were monitored for any histological structural changes. The results revealed no evident changes in the histological structure of the normal knee joint following injection of 1 mg/kg MB at 1, 4, 8 and 24 weeks post-injection. Compared with the vehicle control, MB treatment significantly enhanced the weight distribution and significantly decreased the swelling ratio of the rabbits. Additionally, levels of long non-coding RNA (lncRNA) maternally expressed 3 (MEG3) mRNA were significantly increased following treatment with MB, but the protein expression of P2X purinoceptor 3 (P2X3) was significantly suppressed compared with the vehicle control. The levels of interleukin (IL) 6, tumor necrosis factor (TNF)α, IL-1β and IL-8 were significantly suppressed following MB treatment, indicating that MB protects against OA progression. It was also revealed that MEG3 overexpression significantly suppresses levels of P2X3 protein. ELISA indicated that the MEG3-induced reduction of IL-6, TNFα, IL-1β and IL-8 expression was significantly reversed following P2X3 overexpression. Therefore, the results of the present study demonstrated that MB is an effective method of treating OA-associated pain by upregulating lncRNA MEG3 levels. Additionally, lncRNA MEG3 relieves the OA-associated pain and inflammation in a rabbit model of OA by inhibiting P2X3 expression.

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Section: Discussionmentioning

confidence: 99%

Methylene blue relieves the development of osteoarthritis by upregulating lncRNA MEG3

et al. 2018

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“…Similar to TRPV1, P2X3 receptors in sensory neurons are known to mediate the transmission of nociceptive information, and the levels of both P2X3 receptor protein and mRNA were shown to be significantly upregulated in the rat model of neuropathic pain [14,15]. Down-regulation of P2X3 receptor expression in peripheral tissues and the nervous system is further shown to partially reverse mechanical hyperalgesia following the neuropathic and inflammatory pain [16,29]. In our current study, we discovered that both the P2X3 positive neurons and P2X3 proteins were significantly increased in the spinal cord of BV-administered rats, which was reversed by treating with dl-THP.…”

Section: Discussionmentioning

confidence: 96%

“…Accumulated knowledge from previous studies reveal a crucial role of P2X3 receptor and TRPV1 in the occurrence and maintenance of pain. P2X3 receptor is shown to be upregulated in pathological pain [13][14][15], and its down-regulation can alleviate mechanical hyperalgesia in different experimental pain models [13,16]. TRPV1 also has been shown to mediate thermal hyperalgesia in animal models of inflammatory and neuropathic pain [8,17,18].…”

Section: Introductionmentioning

confidence: 99%

Analgesic effect of dl-THP on inflammatory pain mediated by suppressing spinal TRPV1 and P2X3 receptors in rats

Wang

Sun

et al. 2021

Front. Biosci. (Landmark Ed)

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Introduction 3. Materials and methods 3.1 Animals 3.2 BV pain model and behavioral testing 3.3 Immunohistochemistry 3.4 Western blot analysis 3.5 Experimental drugs 3.6 Data analysis 4. Results 4.1 Effects of dl-THP pre-treatment on the BV-induced persistent spontaneous nociception and pain hyperalgesia 4.2 Effects of post-treatment with dl-THP on the BV-induced pain hyperalgesia. 4.3 Effects of dl-THP on the expression of TRPV1 in the spinal dorsal horn. 4.4 Effects of dl-THP on the expression of P2X3 receptor in the spinal dorsal horn. 4.5 Effects of dl-THP on motor function 5. Discussion 6. Author contributions 7. Ethics approval and consent to participate 8. Acknowledgment 9. Funding 10. Conflict of interest 11. References

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“…[23] Dorn et al [24] showed that pain tolerance increases with a significant decrease in the mRNA level of the P2X 3 receptor in the DRG of mice. Yu et al [25] reported that the protein expression of the P2X 3 receptor was significantly up-regulated in the spinal dorsal horns of rats with chronic compression of dorsal root ganglion (CCD) and that suppressing the protein expression of the P2X 3 receptor alleviated mechanical allodynia. This study demonstrated that rats in CCI group exhibited severe mechanical allodynia and thermal hyperalgesia after sciatic nerve ligation, and this NP was sustained throughout the experiment while it was treated using PRF.…”

Section: Discussionmentioning

confidence: 99%

Pulsed radiofrequency inhibits expression of P2X3 receptors and alleviates neuropathic pain induced by chronic constriction injury in rats

Miao

Ren

et al. 2019

Chinese Medical Journal

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Background:Pulsed radiofrequency (PRF) is a minimally invasive interventional technique that provides a novel and effective treatment strategy for neuropathic pain (NP). PRF is advantageous because it does not damage nerves and avoids sensory loss after treatment. At present, animal studies have demonstrated that PRF is safe and effective for relieving the NP associated with sciatic nerve damage in rats with chronic constriction injury (CCI). However, the mechanism through which this effect occurs is unknown. An increasing body of evidence shows that the expression of the P2X ligand-gated ion channel 3 (P2X3) receptor is closely related to NP; this study was to investigate whether the expression of this receptor is involved in NP relief due to PRF.Methods:A total of 36 healthy adult male Sprague-Dawley (SD) rats were randomly divided into three groups: Sham group, CCI group, and PRF group. The right sciatic nerve was ligated in CCI group and PRF group to establish a CCI model; the right sciatic nerve was separated but not ligated in Sham group. On day 14 after the operation, PRF was administered to the ligated sciatic nerve in PRF group (42°C, 45 V, 2 min). A non-live electrode was placed at the exposed sciatic nerve for the rats in Sham and CCI groups. The hindpaw withdrawal threshold (HWT) and thermal withdrawal latency (TWL) were measured at the right hindpaw at different time points before and after PRF or sham therapy. On day 28 after treatment, the dorsal root ganglion (DRG) and spinal dorsal horn of the right L4–6 were harvested from each group to determine the mRNA and protein levels of the P2X3 receptor.Results:On day 28 after PRF treatment, the HWT (8.33 ± 0.67 g vs. 3.62 ± 0.48 g) and TWL (25.42 ± 1.90 s vs. 15.10 ± 1.71 s) were significantly higher in PRF group as compared to CCI group (P < 0.05). The mRNA expression of the P2X3 receptor in the DRG in PRF group was 23.7% lower than that in CCI group (P < 0.05), in the spinal dorsal horns in PRF group was 22.7% lower than that in CCI group (P < 0.05). The protein expression of the P2X3 receptor in the DRG in PRF group was 27.8% lower than that in CCI group (P < 0.05), in the spinal dorsal horns in PRF group was 35.6% lower than that in CCI group (P < 0.05).Conclusion:PRF possibly reduces NP in CCI rats by inhibiting the expression of the P2X3 receptor in the L4–6 DRG and spinal dorsal horns.

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Pregabalin Alters Nociceptive Behavior and Expression Level of P2X3 Receptor in the Spinal Dorsal Horn in a Rat Model Induced by Chronic Compression of the Dorsal Root Ganglion

Cited by 11 publications

References 34 publications

Methylene blue relieves the development of osteoarthritis by upregulating lncRNA MEG3

Methylene blue relieves the development of osteoarthritis by upregulating lncRNA MEG3

Analgesic effect of dl-THP on inflammatory pain mediated by suppressing spinal TRPV1 and P2X3 receptors in rats

Pulsed radiofrequency inhibits expression of P2X3 receptors and alleviates neuropathic pain induced by chronic constriction injury in rats

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