Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte

Palermo, Gianpiero D.; Joris, Hubert; Devroey, Paul; Steirteghem, A.C. Van

doi:10.1016/0140-6736(92)92425-f

Cited by 3,227 publications

(1,553 citation statements)

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“…The most successful, but also the most invasive, among these techniques is intracytoplasmic sperm injection (ICSI) (1). Present knowledge about the process of fertilization of oocytes in vivo is based predominantly on extrapolation of data from different types of in vitro fertilization studies.…”

Section: ~Troduction Materials and Methodsmentioning

confidence: 99%

Is paternal mitochondrial DNA transferred to the offspring following intracytoplasmic sperm injection?

Houshmand

Holme

Hanson

et al. 1997

J Assist Reprod Genet

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Section: ~Troduction Materials and Methodsmentioning

confidence: 99%

Is paternal mitochondrial DNA transferred to the offspring following intracytoplasmic sperm injection?

Houshmand

Holme

Hanson

et al. 1997

J Assist Reprod Genet

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“…A total of 40 patients undergoing ICSI treatment [29] for male factor infertility were enrolled into a specific ART program. All women were pre-menopausal, 25-45 years of age with a normal hormonal profile according to WHO guidelines.…”

Section: Patient Populationmentioning

confidence: 99%

LH receptor gene expression in cumulus cells in women entering an ART program

Papamentzelopoulou

Mavrogianni

Partsinevelos

et al. 2012

J Assist Reprod Genet

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Purpose Luteinizing hormone (LH) exerts its actions through its receptor (LHR), which is mainly expressed in theca cells and to a lesser extent in oocytes, granulosa and cumulus cells. The aim of the present study was the investigation of a possible correlation between LHR gene and LHR splice variants expression in cumulus cells and ovarian response as well as ART outcome. Methods Forty patients undergoing ICSI treatment for male factor infertility underwent a long luteal GnRH-agonist downregulation protocol with a fixed 5-day rLH pretreatment prior to rFSH stimulation and samples of cumulus cells were collected on the day of egg collection. RNA extraction and cDNA preparation was followed by LHR gene expression investigation through real-time PCR. Furthermore, cumulus cells were investigated for the detection of LHR splice variants using reverse transcription PCR. Results Concerning LHR expression in cumulus cells, a statistically significant negative association was observed with the duration of ovarian stimulation (odds ratio00.23, p00.012). Interestingly, 6 over 7 women who fell pregnant expressed at least two specific types of LHR splice variants (735 bp, 621 bp), while only 1 out of 19 women that did not express any splice variant achieved a pregnancy. Conclusions Consequently, the present study provide a step towards a new role of LHR gene expression profiling as a biomarker in the prediction of ovarian response at least in terms of duration of stimulation and also a tentative role of LHR splice variants expression in the prediction of pregnancy success.

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“…The introduction of intracytoplasmic sperm injection (ICSI) technology [1] has revolutionised the treatment of patients with severe oligozoospermia and azoospermia. A patient is usually considered oligozoospermic if his sperm concentration is less than 20 million mL −1 .…”

Section: Dear Editormentioning

confidence: 99%