Pregnancy-associated glycoprotein and progesterone profiles during pregnancy and postpartum in native goats from the north-east of Brazil

Sousa, Noelita Melo de; Garbayo, J. M.; Figueiredo, José Ricardo de; Sulon, Joseph; Gonçalves, P. B. D.; Beckers, Jean‐François

doi:10.1016/s0921-4488(98)00171-0

Cited by 58 publications

(59 citation statements)

References 21 publications

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“…In cows [31,32] and sheep [33], except for the highest levels on the day of estrus, LH does not vary significantly throughout pregnancy. Additionally, we recorded a concurrent increase in the plasma concentration of progesterone, and this is in agreement with previous reports in pregnant goats [1][2][3][4][5][6][7]. These findings confirm the selective suppressing effect of progesterone on the pituitary secretion of LH through a negative feedback mechanism.…”

Section: Figsupporting

confidence: 93%

Profiles of Circulating Steroid Hormones, Gonadotropins, Immunoreactive Inhibin and Prolactin During Pregnancy in Goats and Immunolocalization of Inhibin Subunits, Steroidogenic Enzymes and Prolactin in the Corpus Luteum and Placenta

Kandiel

Watanabe

Sosa

et al. 2010

Journal of Reproduction and Development

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Abstract. The current study was performed to follow up the circulating hormonal changes and to correlate the findings with the physiological activity of the corpus luteum (CL) and placenta during pregnancy in goats. Blood samples were collected weekly from five goats during pregnancy for measuring steroid and protein hormones. A gradual increase was observed in immunoreactive (ir-) inhibin, with maximal levels at the 17th week. The plasma concentrations of estradiol and prolactin (PRL) showed nearly similar patterns during pregnancy, where they declined to basal levels during the first 4 weeks post-breeding and then increased significantly, with the maximal concentration during late pregnancy. The plasma FSH and LH concentrations were maintained at basal levels throughout the gestation period. The plasma progesterone concentration abruptly increased in the first week post-breeding and remained at high values throughout the pregnancy period. Immunohistochemical localization of inhibin alpha, betaA, betaB and steroidogenic enzymes cytochrome P450 aromatase, 3alpha-hydroxysteroid dehydrogenase (3betaHSD), cytochrome 17alpha-hydroxylase P450 and cholesterol side-chain cleavage cytochrome P450 in the cyclic and pregnant goat CL revealed positive immunoreactivity without affinity differences between the luteal and pregnancy stages. The placental syncytiotrophoblasts also showed positive staining, except for inhibin betaA and 3betaHSD. The giant binucleate cells of the placenta showed positive immunoreactions to PRL. These results suggest that the high concentrations of irinhibin, estradiol and PRL during late pregnancy are of placental origin and that the placenta may have a vital role in the maintenance of pregnancy, regulation of mammary growth and preparation for kidding and lactation in goats. Key words: Goat, Hormone, Inhibin, Placenta, Pregnancy (J. Reprod. Dev. 56: [243][244][245][246][247][248][249][250] 2010) tudying hormonal changes during the gestation period knocks down doors that block understanding of the regulatory mechanisms accompanying fetal development and maintenance of pregnancy; the most important stage of animal reproductive life. In goats, pregnancy is dependant on the corpus luteum (CL) with minimal contribution from placenta. However, day by day, scientists are working hard to discover the additional roles played by the placenta. The pattern of progesterone levels during pregnancy is changeable; it is low from mating until the 6th day [1], increases noticeably by the 3rd week and remains high until the 19th week [2][3][4][5][6][7]. Maintenance of progesterone secretion by the CL during pregnancy in the goat is luteinizing hormone (LH)-dependant; moreover, prolactin (PRL) is synergistic with LH in stimulating this function [8]. The highest concentration of estradiol in the blood is found 2 days before ovulation [1,9]; the concentration decreases during the first 30 days after mating and then increases again between weeks 7 and 11, with the maximal output near parturition [7,10].Up till now,...

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Section: Figsupporting

confidence: 93%

Profiles of Circulating Steroid Hormones, Gonadotropins, Immunoreactive Inhibin and Prolactin During Pregnancy in Goats and Immunolocalization of Inhibin Subunits, Steroidogenic Enzymes and Prolactin in the Corpus Luteum and Placenta

Kandiel

Watanabe

Sosa

et al. 2010

Journal of Reproduction and Development

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“…The assay of steroid hormone concentrations (progesterone, estrone sulfate) at a determined time after insemination/mating aids the early detection of pregnancy (Worsfold et al, 1986;Murray & Newstead, 1988;Refstal et al, 1991;Sousa et al, 1999).…”

Section: Hormonal Assaysmentioning

confidence: 99%

Pregnancy diagnosis techniques in goats – a review

Karadaev¹

2015

BJVM

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SummaryKaradaev, M., 2015. Pregnancy diagnosis techniques in goats -a review. Bulg. J. Vet. Med., 18, No 3,[183][184][185][186][187][188][189][190][191][192][193] Several techniques for pregnancy diagnosis in goats are developed. Some of them are efficient and applicable in the field, but not enough accurate. Others are outlined with a high precision, but require equipment and specialised skills. Laparoscopy, laparotomy and vaginal biopsy are accurate but invasive and thus, inappropriate for the routine practice. Abdominal inspection, transabdominal palpation and increased live weight could be indicative for pregnancy in goats but they are reliable only after the second half of gestation. Udder examination and palpation are of low accuracy and their independent application for pregnancy diagnosis is not suitable. Blood, milk and faecal progesterone assays could indicate pregnancy in goats after the 21 st post insemination day until the end of gestation. The detection of pregnancy associated glycoproteins (PAGs) in blood plasma or milk samples is an early pregnancy marker but requires laboratory equipment. Non-return to oestrus is a cheap, practical and widely used method for detection of pregnancy between post insemination days 17 and 21 and does without signs of oestrus are assumed to be pregnant. A-mode, B-mode and Doppler ultrasonography are alternative methods for pregnancy diagnosis. Their accuracy and practical application potential are different. The two-dimensional ultrasound is one of the most appropriate methods for pregnancy diagnosis in goats. The accuracy of the method is about 100% on post insemination day 25-30 using the transrectal approach, whereas via the transabdominal approach, this accuracy is attained between the 40 th and the 45 th day.

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“…Chez la brebis n° 1 007, les concentrations en progestérone sont restées faibles pendant les cinq premières semaines de la gravidité, pouvant ainsi suggérer une synthèse insuffisante de progestérone comme étant à l'origine de la mortalité embryonnaire. Ces résultats vont dans le même sens que ceux signalés suite aux dosages de la Pspb et de la progestérone chez les brebis (32) et les chèvres (13), ainsi que de la PAG et de la progestérone chez les chèvres (34, 35) et chez les vaches (25). Ces travaux confirment que si le dosage de la progestérone (17 e et 21 e jours de gravidité) ou de la PAG (à partir du 28 e jour) permettent de diagnostiquer une gravidité, leur combinaison, en revanche, permet d'identifier les pertes embryonnaires et d'estimer le moment de leur survenue (4,12,24,25,26).…”

Section: Identification Des Différents éVénements Physiopathologiquesunclassified

“…Ces résultats vont dans le même sens que ceux signalés suite aux dosages de la Pspb et de la progestérone chez les brebis (32) et les chèvres (13), ainsi que de la PAG et de la progestérone chez les chèvres (34, 35) et chez les vaches (25). Ces travaux confirment que si le dosage de la progestérone (17 e et 21 e jours de gravidité) ou de la PAG (à partir du 28 e jour) permettent de diagnostiquer une gravidité, leur combinaison, en revanche, permet d'identifier les pertes embryonnaires et d'estimer le moment de leur survenue (4,12,24,25,26). De ce fait, ils permettent de différencier plus précocement des échecs de fécondation, suite à l'insémination artificielle ou à la monte naturelle, de ceux dus à des mortalités embryonnaires.…”

Section: Identification Des Différents éVénements Physiopathologiquesunclassified

“…De même, le déclin des concentrations a pré-cédé l'expulsion du foetus. Des profils similaires suite à des avortements ont été décrits chez la vache (4,12,24,26), la chèvre (7,12,25,34,35) et la brebis (16,32). Des études complémentaires sont nécessaires pour établir si l'altération de la fonction trophoblastique a été la cause ou la conséquence de la détresse et de la mort foetale.…”

Section: Identification Des Différents éVénements Physiopathologiquesunclassified

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Suivi de la gravidité chez la brebis Ouled Djellal par dosage de la protéine associée à la gestation et de la progestérone

Benyounes

Lamrani²,

Sousa

et al. 2006

Rev. Elev. Med. Vet. Pays Trop.

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L’objectif de la présente étude a été la caractérisation des profils plasmatiques de la protéine associée à la gestation (PAG) et de la progestérone, au cours de la gravidité et deux semaines après le part chez la race Ouled Djellal. Vingtcinq brebis ont été prélevées hebdomadairement après synchronisation des chaleurs et monte naturelle. Les concentrations de PAG et de progestérone ont été déterminées par dosage radio-immunologique. Deux femelles n’ont pas été fécondées tandis que 23 ont eu des gestations considérées comme normales (n = 18) ou pathologiques (n = 5). Chez 18 brebis (12 gestations simples et 6 multiples), les concentrations de PAG et de progestérone ont été significativement influencées par le stade de la gestation (P < 0,0001) et non par le nombre de foetus. Des profils individuels ont été décrits chez sept brebis, dont trois femelles ayant eu des pertes embryonnaires, et deux brebis ayant avorté (66e et 110e jours), et enfin chez deux brebis ayant donné naissance à terme chacune à un agneau vivant et expulsé au moment de la mise bas un ou deux foetus momifiés. Les concentrations en PAG ont été plus faibles chez les femelles ayant eu des foetus momifiés en parallèle avec des foetus vivants.

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Pregnancy-associated glycoprotein and progesterone profiles during pregnancy and postpartum in native goats from the north-east of Brazil

Cited by 58 publications

References 21 publications

Profiles of Circulating Steroid Hormones, Gonadotropins, Immunoreactive Inhibin and Prolactin During Pregnancy in Goats and Immunolocalization of Inhibin Subunits, Steroidogenic Enzymes and Prolactin in the Corpus Luteum and Placenta

Profiles of Circulating Steroid Hormones, Gonadotropins, Immunoreactive Inhibin and Prolactin During Pregnancy in Goats and Immunolocalization of Inhibin Subunits, Steroidogenic Enzymes and Prolactin in the Corpus Luteum and Placenta

Pregnancy diagnosis techniques in goats – a review

Suivi de la gravidité chez la brebis Ouled Djellal par dosage de la protéine associée à la gestation et de la progestérone

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