Pregnancy-associated glycoprotein (PAG) pattern and pregnancy detection in Boer goats using an ELISA with different antisera

Shahin, M; Friedrich, M; Gauly, Matthias; Beckers, Jean‐François; Holtz, W.

doi:10.1016/j.smallrumres.2013.01.016

Cited by 15 publications

(18 citation statements)

References 21 publications

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“…According to Gonzalez et al (2004) detectable blood plasma PAGs concentrations in goats appear around the 25-26 day of gestation. Shahin et al (2013) reported statistically significant differences in PAGs levels by the 21 st day post breeding between pregnant and nonpregnant goats. The presence of PAGs in milk samples is also a precise criterion for pregnancy after the 32 nd gestation day (Gonzalez et al, 2004).…”

Section: Pregnancy Associated Glycoproteinsmentioning

confidence: 91%

“…The detection of pregnancy associated glycoproteins (PAGs) in blood plasma or milk samples is a reliable method for early pregnancy diagnosis in goats (Batalha et al, 2011;Zamfirescu et al, 2011;Shahin et al, 2013). According to Gonzalez et al (2004) detectable blood plasma PAGs concentrations in goats appear around the 25-26 day of gestation.…”

Section: Pregnancy Associated Glycoproteinsmentioning

confidence: 99%

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Pregnancy diagnosis techniques in goats – a review

Karadaev¹

2015

BJVM

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SummaryKaradaev, M., 2015. Pregnancy diagnosis techniques in goats -a review. Bulg. J. Vet. Med., 18, No 3,[183][184][185][186][187][188][189][190][191][192][193] Several techniques for pregnancy diagnosis in goats are developed. Some of them are efficient and applicable in the field, but not enough accurate. Others are outlined with a high precision, but require equipment and specialised skills. Laparoscopy, laparotomy and vaginal biopsy are accurate but invasive and thus, inappropriate for the routine practice. Abdominal inspection, transabdominal palpation and increased live weight could be indicative for pregnancy in goats but they are reliable only after the second half of gestation. Udder examination and palpation are of low accuracy and their independent application for pregnancy diagnosis is not suitable. Blood, milk and faecal progesterone assays could indicate pregnancy in goats after the 21 st post insemination day until the end of gestation. The detection of pregnancy associated glycoproteins (PAGs) in blood plasma or milk samples is an early pregnancy marker but requires laboratory equipment. Non-return to oestrus is a cheap, practical and widely used method for detection of pregnancy between post insemination days 17 and 21 and does without signs of oestrus are assumed to be pregnant. A-mode, B-mode and Doppler ultrasonography are alternative methods for pregnancy diagnosis. Their accuracy and practical application potential are different. The two-dimensional ultrasound is one of the most appropriate methods for pregnancy diagnosis in goats. The accuracy of the method is about 100% on post insemination day 25-30 using the transrectal approach, whereas via the transabdominal approach, this accuracy is attained between the 40 th and the 45 th day.

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Section: Pregnancy Associated Glycoproteinsmentioning

confidence: 91%

Section: Pregnancy Associated Glycoproteinsmentioning

confidence: 99%

Pregnancy diagnosis techniques in goats – a review

Karadaev¹

2015

BJVM

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“…Thereafter, caPAGs concentration starts to decrease at the 9th week of pregnancy, reaching 16-32 ng/ mL around between the 12th and 17th weeks, and then decreases slowly until parturition. After parturition, caPAGs concentrations decrease rapidly, and continue to decrease until the 4th week post parturition (Humblot et al, 1990;Garbayo et al, 1998;Shahin et al, 2013). Thereby, determination of caPAGs may have a considerable diagnostic merit in Aardi goats.…”

Section: Resultsmentioning

confidence: 99%

“…The appearance of small quantities of binucleate cells and these novel antigen-specific proteins for pregnancy are observed around 18 days after mating in goats and significantly the value of such screening measure lies in its capacity to discriminate between does those that are pregnant and those with extended inter-estrous intervals or non-pregnant as early as Day 21 after breeding, which cannot be determined either by estrous behavior, P4 assay, or US technique. Moreover, the test has the potential to be highly suitability for field practice (Shahin et al, 2013). Nevertheless, to our knowledge, studies on the diagnostic reliability of caPAGs in detecting early pregnancy in Aardi goats are lacking.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic reliability of enzymatic-immunoassayed serum pregnancy-associated glycoproteins in detecting early pregnancy compared to other screening measures in Aardi goats (Capra hircus) in saudi arabia

Al-Samawi

Al-Hassan

Swelum

2015

IJAR

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Despite proven ability of pregnancy-associated glycoproteins (PAGs) for early detection of pregnancy in several livestock, studies on its diagnostic reliability in Aardi goats are lacking. Thirty-two healthy mature Aardi does, managed under an intensive condition, were screened for pregnancy at 1, 7, 15, 23, 35 and 60 days post natural mating (NM) to evaluate the ability of utilizing caprine pregnancy-associated glycoproteins (caPAGs) compared to other methods frequently appliedbut have several setbacks-in screening goats for early pregnancy diagnosis. According to the enzymatic-immunoassay data, our results indicated that pregnant Aardi does had higher (P < 0.0001) serum caPAGs concentration than their nonpregnant counterparts after 23, 35 and 60 days post NM. Therefore, determination of serum caPAGs may have a considerable diagnostic merit in Aardi goats. To confirm, the ability of caPAGs in detecting early pregnancy was then compared with enzymatic-immunoassayed serum progesterone (P4) assay as well as trans-rectal and abdominal ultrasonography (US) technique. The present data revealed that there were no differences (P > 0.05) between the sensitivity, specificity, predictive values, and accuracy of serum caPAGs and other methods as early as day 23 post NM. Moreover, both serum caPAGs and US technique were proven to give higher (P < 0.0001) sensitivity, specificity, predictive values, and accuracy than serum P4 at 35 days post NM. Thereby, this implies that serum caPAGs can be used alone or in combination with other methods for early pregnancy diagnosis in Aardi goats. In conclusion, the obtained results herein clearly demonstrated that enzymaticimmunoassayed serum caPAGs, as a direct, simple, accurate, inexpensive, practical and fast screening measure, was greatly feasible for distinguishing pregnant from non-pregnant as early as day 23 post NM, which is crucial to treat fertility problems early and efficiently.

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“…With the antibody rose against caprine PAG a steep increase to a peak level of 69±9ng/ml on day 56 of pregnancy was followed by a gradual decline to 16±3 ng/ml at parturition and 0.3±0.07 ng/ml four weeks postpartum. With antibovine PAG, the PAG level increased to a maximum of 3.1±0.2 ng/ ml on day 105 of pregnancy and fluctuated around 3ng/ml until the end of pregnancy suggesting its ability to diagnose early pregnancy and its possible use to evaluate feto-placental well-being [60,61].…”

Section: Pregnancy Associated Glycoprotein (Pag)mentioning

confidence: 99%