Pregnancy by the tubal transfer of embryos developed after injection of round spermatids into oocyte cytoplasm of the cynomolgus monkey (Macaca fascicularis)

Ogonuki, Narumi; Tsuchiya, Hideaki; Hirose, Yoshihiro; Okada, Hiromasa; Ogura, Atsuo; Sankai, Tadashi

doi:10.1093/humrep/deg212

Cited by 28 publications

(18 citation statements)

References 41 publications

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“…Intracytoplasmic injection of round spermatids (ROSI) also produced normal offspring [13]. Today, animal species in which live offspring were obtained by ROSI include mice [12,13], mastomys [14], rats [15], rabbits [16], monkeys [17], and humans [18]. We report here the birth of hamster offspring following ROSI.…”

Section: Introductionmentioning

confidence: 96%

Full-Term Development of Hamster Embryos Produced by Injection of Round Spermatids into Oocytes

Haigo

Yamauchi

Yazama

et al. 2004

Biology of Reproduction

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The golden hamster is a mammal in which microinjection of round spermatids into oocytes (ROSI) was first attempted. However, no live ROSI offspring have ever been obtained in this species. This is the first report of live hamster offspring obtained by round spermatid injection. Over 90% of oocytes, injected with round spermatids, were activated without any additional stimulation. The proportion of the oocytes that were fertilized normally and that developed to morulae and blastocysts was higher when the plasma membranes of the spermatids were broken before injection, as compared with when the membranes were left intact. Five percent of 57 ROSI morulae/blastocysts developed into live offspring after transfer to foster mothers.

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Section: Introductionmentioning

confidence: 96%

Full-Term Development of Hamster Embryos Produced by Injection of Round Spermatids into Oocytes

Haigo

Yamauchi

Yazama

et al. 2004

Biology of Reproduction

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“…Since the first birth of a rhesus macaque after IVF in the mid-1980s (1), several laboratories have reported successfully obtaining infants of rhesus, cynomolgus monkey, and pigtailed macaque by IVF or ICSI (8)(9)(10)(11)(12)(13)(14). However, the success rate has been low in comparison with that for rodents and humans.…”

Section: Discussionmentioning

confidence: 99%

“…To date there has been no report using a pronuclear embryo transfer technique for the production of in vitro-fertilized nonhuman primates, including cynomolgus monkey (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14). Based on our and other researchers' extensive experiences in producing transgenic mice (15,(18)(19)(20)(21)(22), it is reasonable to postulate that transferring embryos at the pronuclear stage can come with the certain benefits, such as decreasing the risk of losing embryo quality during the course of long-term in vitro culture.…”

Section: Discussionmentioning

confidence: 99%

Efficient reproduction of cynomolgus monkey using pronuclear embryo transfer technique

Sun

Dong

Yang

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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One of the technical bottlenecks in producing nonhuman primate models is that current assisted reproductive techniques, such as in vitro culture and frozen conservation of multicell-stage embryos, often result in poor embryo quality and subsequently lead to low birth rates. We investigated whether pronuclear embryo transfer can be used as an effective means for improving pregnancy and live birth rates of nonhuman primates. We collected 174 metaphase II oocytes by laparoscopy from 22 superovulated mature females and then fertilized these eggs using either in vitro fertilization or intracytoplasmic sperm injection, resulting in a 33.3% and a 50% fertilization rate, respectively. These 66 fertilized pronuclear-stage embryos were then tubally transferred to 30 recipients and led to 7 births and 1 abortion. Importantly, we observed that the highest live birth rate of Ϸ64% was obtained when the transfer of pronuclear embryos was performed in the presence of new corpus luteum in the ovary of recipients between 24 h and 36 h after estradiol peak. Therefore, our experiments demonstrate that by matching the critical time window in the recipient's reproductive cycle for achieving optimal embryo-uterine synchrony, pronuclear embryo transfer technology can significantly improve the pregnancy rate and live birth of healthy baby monkeys. This efficient method should be valuable to the systematic efforts in construction of various transgenic primate disease models.in vitro fertilization ͉ intracytoplasmic sperm injection ͉ test-tube monkey ͉ transgenic monkey ͉ embryo-uterine synchrony C onsidering the great genetic and physiologic similarities with humans, nonhuman primates such as monkeys represent the most ideal experimental models for detailed analysis of biologic processes under physiologic or pathologic conditions. As a result, new drug candidates are typically required to go through systematic assessment of drug efficacy, pharmacokinetics, and toxicity in monkeys before their evaluation in human clinical trials. Furthermore, monkeys are the most favored organisms for in-depth analysis of neural mechanisms underlying high cognition and complex behavior. It is conceivable that the potential of the nonhuman primate model systems can be further expanded when researchers introduce or remove genes of interest by transgenic methods that have been proven so powerful in lower organisms such as mice, zebra fish, and fruit flies.One of the major bottlenecks in producing transgenic monkeys is the low birth rate of ''test-tube'' baby monkeys with assisted reproductive technologies. Although the first birth of a monkey (a rhesus macaque) after in vitro fertilization (IVF) was reported in 1984 (1), constraints on materials and resources for systematic experimentation with various in vitro culture conditions and procedures have contributed to the slow progress of the field. There are also many factors that can greatly affect the quality of embryo development and the rates of pregnancy and live birth. For example, the success of IVF meth...

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“…Several normal clinical pregnancies following round spermatid injection have been reported (Antinori et al 1997, Kahraman et al 1998, Barak et al 1998, although disappointingly poor fertilization and subsequent embryonic development are common outcomes (Levran et al 2000, Vicdan et al 2001. Fertilization of cynomolgus macaque oocytes with round spermatids has been reported; however, the only pregnancy established aborted at day 103 (Ogonuki et al 2003). Round spermatids differ biologically from mature sperm in several important ways, such as immaturity of certain cytoplasmic and nuclear proteins (Ziyyat & Lefevre 2001), which probably contributes to the poor pregnancy rates (Ghazzawi et al 1999).…”

Section: Cytoskeletal Architecture During Primate Icsimentioning

confidence: 99%

Primate models for assisted reproductive technologies

Hewitson¹

2004

Reproduction

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Although the deliberate creation of human embryos for scientific research is complicated by ethical and practical issues, a detailed understanding of the cellular and molecular events occurring during human fertilization is essential, particularly for understanding infertility. It is clear from cytoskeletal imaging studies of mouse fertilization that this information cannot be extrapolated to humans because of unique differences in centrosomal inheritance. However, the cytoskeletal rearrangements during non-human primate fertilization are very similar to humans, providing a compelling animal model in which to examine sperm-egg interactions. In order to address this key step in primate fertilization and to avoid the complexities in working with fertilized human zygotes, studies are now exploring the molecular foundations of various assisted fertilization techniques in a monkey model. While intracytoplasmic sperm injection with ejaculated or testicular sperm is quite successful in primate models, there are some specific differences when compared with standard IVF that warrant further investigation, particularly in regards to nuclear remodeling, genomic imprinting, Y-chromosome deletions and developmental outcomes. Similarly, primate models have been useful for examining spermatid function during fertilization but these have met with limited success. One area of primate reproductive research that has yet to be mastered is reproductive cloning. Genetically identical primates would provide the ultimate approach for accelerating stem cell-based therapies for a number of neurodegenerative diseases such as Alzheimer's and Parkinson's disease, as well as targeted gene therapies for various metabolic disorders.

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Pregnancy by the tubal transfer of embryos developed after injection of round spermatids into oocyte cytoplasm of the cynomolgus monkey (Macaca fascicularis)

Cited by 28 publications

References 41 publications

Full-Term Development of Hamster Embryos Produced by Injection of Round Spermatids into Oocytes

Full-Term Development of Hamster Embryos Produced by Injection of Round Spermatids into Oocytes

Efficient reproduction of cynomolgus monkey using pronuclear embryo transfer technique

Primate models for assisted reproductive technologies

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