Pregnancy percentage following deposition of sex-sorted sperm at different sites within the uterus in estrus-synchronized heifers

Kurykin, J.; Jaakma, Ülle; Jalakas, M.; Aidnik, Madis; Waldmann, A.; Majas, L.

doi:10.1016/j.theriogenology.2006.10.006

Cited by 23 publications

(18 citation statements)

References 28 publications

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“…The proportion of live female calves after confirmation was 93% in this experiment. This result was similar to the proportion of females (93.2%) following the use of sex-sorted semen in the estrus synchronization program in the experiment of Kurykin et al (2007). Other studies showed that the proportion of females following the use of sexed AI in lactating cows can reach 90% (DeJarnette et al 2009; Norman et al 2010).…”

Section: Discussionsupporting

confidence: 85%

“…In a study by Kurykin et al (2007), estrus synchronization in heifer insemination was conducted using approximately 2.2 million sperm per straw of sex-sorted semen in the UB, UUH, and utero-tubal junction. In their experiment, heifers exhibiting strong signs of estrus reached 45.9%, but weak signs of estrus only reached 20.8% of pregnancy rate.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that the deposition sites and estrus status can greatly affect the outcome of sexed AI. Kurykin et al (2007) reported that pregnancy rates are higher than 100% when the animals are in strong estrus (45.9%) than when they are in weak estrus (20.8%).…”

Section: Introductionmentioning

confidence: 99%

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Artificial insemination of Holstein heifers with sex-sorted semen during the hot season in a subtropical region

Chang

Chou

Shiu

et al. 2017

Trop Anim Health Prod

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Our aim was to investigate insemination techniques in order to improve pregnancy rates of artificial insemination (AI) using sex-sorted semen (sexed AI) in cattle in tropical and subtropical (T/ST) regions. In T/ST regions, the pregnancy rates by sexed AI are reportedly the lowest in the hottest months of the year, with less than 15% in cows and 35–40% in heifers (PMID 24048822). We compared sexed AI by depositing the semen into the uterine body (UB-AI, n = 12) versus the unilateral uterine horn (UUH-AI, n = 14) of pre-ovulation heifers. The ovary and follicle were assessed by rectal ultrasound before AI. After insemination, pregnancy was determined by ultrasound at approximately 40 days and approximately 70 days. In the present study, we demonstrated that high pregnancy rates (>70%) by sexed AI in the hottest season in a subtropical region such as Taiwan can be achieved when heifers with pre-ovulation follicles are used. The overall pregnancy rates were 54% higher in the UUH-AI (71%) group than in the UB-AI (42%) group (P = 0.06), examined on approximately 40 days post-sexed AI. Surprisingly, however, the pregnancy outcome appeared to be higher in the hot season (62%) than in the cool season (46%) although this difference was not statistically significant. Based on the present study, we recommend that cattle breeders perform UUH-AI using sex-sorted semen for heifers with pre-ovulation follicles in order to achieve satisfactory pregnancy outcome in the hot seasons in T/ST regions.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

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Artificial insemination of Holstein heifers with sex-sorted semen during the hot season in a subtropical region

Chang

Chou

Shiu

et al. 2017

Trop Anim Health Prod

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“…Wpływ miejsca zdeponowania nasienia na wyniki zacieleń był przedmiotem wielu badań (5,6,9,11,12). Część z nich poświęcona jest efektywności superowulacji, mierzonej liczbą zarodków przydatnych do transferu po zastosowaniu nasienia sortowanego, o zmniejszonej w porównaniu do mrożonego nasienia konwencjonalnego liczbie plemników w dawce.…”

Section: Wyniki I Omówienieunclassified

Influence of semen placement location and other factors on the number and morphological status of obtained embryos from donor cows

Żychlińska-Buczek¹,

Jaśkowski²,

Gil³

et al. 2016

Medycyna Weterynaryjna

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Przenoszenie zarodków ze względu na swoje walory znajduje zastosowanie w nowoczesnej hodowli, szczególnie w hodowli bydła. Obok inseminacji jest biotechniką rozrodu znacznie przyspieszającą postęp hodowlany. Metoda ta rozszerza możliwości wynikające z inseminacji, a w związku z zastosowaniem zaawansowanej techniki, wymaga zaangażowania bardziej wykwalifikowanego personelu, specjalistycznego sprzętu i odpowiedniego przygotowania zwierząt.W rezultacie -w porównaniu do inseminacji -jest to metoda biotechniczna o wiele droższa.Efektywność przenoszenia zarodków uwarunkowana jest wieloma czynnikami. Jednym z istotnych czynników mających wpływ na opłacalność tej metody jest liczba pozyskiwanych zarodków. Zależy ona m.in. od miejsca depozycji nasienia w drogach rodnych dawczyni. Na ogół uważa się, że głębokie, dorogowe wprowadzenie nasienia (krzywizna duża macicy) lub Efektywność pozyskiwania zarodków w zależności od miejsca deponowania nasienia w rui indukowanej u krów dawczyń

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“…(64). W takich przypadkach elementem decydującym jest głębokość wprowadzenia pistoletu do macicy, w okolicę cieśni maciczno-jajowodowej (37,53). Uzasadniona wydaje się głęboka depozycja nasienia sortowanego do ipsilateralnego rogu (63).…”

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Sex-sorted sperm in farm animals: scale of use, the effectiveness of insemination, risk factors

Jaśkowski

Woźna

Gehrke

2017

Medycyna Weterynaryjna

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The aim of the study was to present the scale of use, risk factors and possibilities, which sorter semen gives in biotechnics used in reproduction of cattle. Modern sorters allow for the evaluation of 6 million X and Y spermatozoa per hour. Sex-sorted semen, which is commercially used, contains 2.1 x 106 of spermatozoa. It is used mostly in AI of milk heifers, mainly in large cattle herds. Sorted semen containing Y spermatozoa is sold less often in the world than the one with X spermatozoa. The percentage of the desired sex of the young is higher than 90. The pregnancy rate after application of sorted semen is about 20–25% lower than after insemination of non-sorted semen and depends on a number of factors. The main factors are: breed of female, service number, the herd of origin, the depth of semen deposition, the bull producing semen, ambient temperature and technical parameters during sperm sorting. A number of methods have been developed to improve conception rate, including timed artificial insemination (TAI) and synchronization of heat and ovulation. Results of donor inseminations with the use of sorter semen are presented, with the lower percentage of embryos suitable for the transfer and embryos of the highest quality highlighted. Previous studies do not indicate a reduction of the conception rate after the transfer of embryos obtained in vitro and in vivo after fertilization using sorted semen. It remains difficult to justify a significant increase in the frequency of stillbirths of bulls after using sorted sperm. Similarly, 16% of stillbirths of bulls were observed after embryo transfer, when donors were inseminated with sorter semen. The percentage of stillbirths of bulls after embryo transfer with the use of conventional semen is 9%. The sorted semen is not often used for inseminations in pigs, sheep and goats.

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Pregnancy percentage following deposition of sex-sorted sperm at different sites within the uterus in estrus-synchronized heifers

Cited by 23 publications

References 28 publications

Artificial insemination of Holstein heifers with sex-sorted semen during the hot season in a subtropical region

Artificial insemination of Holstein heifers with sex-sorted semen during the hot season in a subtropical region

Influence of semen placement location and other factors on the number and morphological status of obtained embryos from donor cows

Sex-sorted sperm in farm animals: scale of use, the effectiveness of insemination, risk factors

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