Pregnancy rates following intra-uterine insemination with pellet or straw-frozen ram semen

Hunton, J. R.; Flecker, Sally E.; Maxwell, W.M.C.

doi:10.1017/s0021859600081144

Cited by 15 publications

(5 citation statements)

References 7 publications

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“…The obtained better post-thawed motility and plasma membrane integrity of ram spermatozoa in straws than in pelleted form proved in our study were also reported in goats (Bezerra et al, 2012), boar (Dai et al, 2009). Also, Hunton et al (1987) indicated that semen frozen in straws was more motile than pelleted semen, but both applied cryopreservation methods (pellets and straws) caused a decrease in the percentage of sperm livability. Pickett and Berndtson (1974) reported that superior sperm viability, greater storage efficiency, and higher conception rates resulting from storage in plastic straws have led to rapid increases in the use of this packaging technique.…”

Section: Discussionsupporting

confidence: 83%

Effect of Some Alternative Components of Egg Yolk in Tris-Extender on Sperm Characteristics of Ram Semen Frozen with Two Methods of Packaging Semen

Abdel-Khalek

Khalil

El-Saidy

et al. 2018

Journal of Animal and Poultry Production

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This study aimed to evaluate the effect of alternative components of egg yolk in Tris extender and semen packaging methods during cryopreservation on sperm characteristics and antioxidant system in seminal plasma of frozen-thawed semen of Finnish Landrace rams. Semen was collected from 5 sexually matured Finnish rams (50-70 kg LBW and 2-4 years old) by artificial vagina once weekly for 7 weeks. Only semen with mass motility of ≥70% was pooled and diluted with Tris-citric extender containing 15% egg yolk (Tris-EY) or 1% soybean lecithin (Tris-SBL) or 2 mM butylated hydroxytoluene (Tris-BHT).Semen was extended at a rate of 1:5 (semen/extender) with three extender types. After semen extension, semen was placed for cooling in the refrigerator (5 o C) for 4 hours as equilibration period and packaged in 0.25 ml French straws or 0.25 ml pellets in liquid nitrogen. Semen was thawed at 37°C for 30 s. Semen was evaluated after dilution, equilibration and thawing, for progressive motility, livability and abnormality of spermatozoa and curled tail spermatozoa responded to a solution of osmolarity of 75 mOsm for 30 min. The concentration of total antioxidants (TAC), malondialdehyde (MDA) and lactic dehydrogenase (LDH) activity in post-thawed seminal plasma were determined. Results showed that sperm characteristics, including percentages of progressive motility, livability, abnormality and curled tail in post-diluted, post-equilibrated or post-thawed semen were not affected significantly by the type of extender. Progressive motility and curled tail percentages in post-thawed semen were higher (P<0.001; P<0.05) in straws than in pellets. Livability and abnormality percentages were insignificantly better at straws than in pellets. The recovery rate of motility and livability was higher at straws than in pellets. All sperm characteristics indicated insignificant effect of interaction between type of extender and semen packaging method. TAC was higher (P<0.05), while MDA concentration was lower (P<0.05) in Tris-SBL and Tris-BHT than in Tris-EY. The activity of LDH was insignificantly the highest in Tris-BHT than in other extenders. The TAC, MDA concentration and LDH activity in post-thawed semen were not affected significantly by semen packaging method. Effects of interaction between type of extender and semen packaging method on each of TAC, MDA concentration and LDH activity were not significant. The current study concluded the successful usage of Tris-SBL or Tris-BHT in comparing with the possible disadvantages of using egg yolk in Tris-based extender of ram semen.

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Section: Discussionsupporting

confidence: 83%

Effect of Some Alternative Components of Egg Yolk in Tris-Extender on Sperm Characteristics of Ram Semen Frozen with Two Methods of Packaging Semen

Abdel-Khalek

Khalil

El-Saidy

et al. 2018

Journal of Animal and Poultry Production

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“…In our study, AI was performed in the ewes selected one by one from MAP-, FGA-, CIDR-and P sponge-treated groups in order during the period of 44 to 52 h after removal of the intravaginal devices. The range of the fixed-time AI with frozen semen in the ewes treated with MAP or FGA sponge with eCG treatment at the time of sponge removal is generally 48 to 60 h [3,4,[27][28][29][30]. Nevertheless, when an eCG injection was given one day before the removal of the intravaginal sponges as in the present study, estrus was induced earlier than when the injection was given at the time of sponge removal [19,20].…”

Section: Discussionmentioning

confidence: 55%

Comparison of Fertility of Estrous Synchronized Ewes with Four Different Intravaginal Devices during the Breeding Season.

et al. 1999

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Comparative studies of four different intravaginal devices containing progesterone or synthetic progestogens on estrous incidence, LH surge and fertility of synchronized ewes by an intrauterine insemination with frozen semen by laparoscopy were conducted during the breeding season. A total of 161 Suffolk or Suffolk-crossed ewes at two sheep farms (Farms A and B) were used. In Farm A, the mean times of estrous onset after the removal of four intravaginal devices were 27.3, 31.2, 21.8 and 22.8 h for MAP, FGA, CIDR and P sponge (self-made progesterone sponge), respectively. The progesterone-impregnated intravaginal devices (CIDR and P sponge) induced estrus significantly earlier (P<0.05) than the devices impregnated with the synthetic progestogens (MAP and FGA) (22.3 ± 2.0 and 29.4 ± 2.3 h, respectively, after the removal of the intravaginal device). CIDR induced estrus (P<0.01) and LH surge (P<0.05) significantly earlier than FGA and MAP sponges, respectively. Pregnancy determined by the plasma progesterone concentration (>2.0 ng/ml) on Day 21 (Farm B) and lambing rates (Farms A and B) of ewes inseminated once on a fixed-time basis between 44 and 52 h after removal of intravaginal devices were not significantly different: 45.0, 41.5, 57.9 and 39.5% in lambing rates for MAP, FGA, CIDR and P sponge, respectively. Also, no significant differences in prolificacy were observed among the four intravaginal devices. These results indicate that the fertility of ewes treated with the four different intravaginal devices including a self-made P sponge and inseminated with frozen semen is similar, although the onset time of estrus and the LH surge varies with the use of progesterone-or progestogen-impregnated intravaginal devices.

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“…Although the lower post-thawing motility of strawfrozen spermatozoa would suggest a reduction in the number of viable spermatozoa in the inseminate, we previously found no difference in the fertility of semen frozen by the straw and pellet methods when the number of motile spermatozoa was reduced to 5 x 10" (Ritar et al 19906). Similar findings in sheep led Hunton et al (1987) to suggest that it may be of questionable value to adjust the number of spermatozoa inseminated on the basis of post-thawing motility. However, they possibly did not reach a threshold where the number of motile spermatozoa may be critical to maintain fertility.…”

Section: Resultsmentioning

confidence: 70%

Fertility of young Cashmere goats after laparoscopic insemination

Ritar¹,

Ball²

1991

J. Agric. Sci.

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SUMMARYMaiden 18-month-old Cashmere does (JV=254) were inseminated laparoscopically with frozenthawed semen after treatment with Controlled Internal Drug Release devices and pregnant mare's serum gonadotrophin (PMSG) in the breeding season of 1989 in Tasmania. Kidding rates(does kidded/inseminated) were not statistically different after the insemination of 1, 5 or 25 × 106 motile spermatozoa (50·0, 55·8 and 56·4%), andfor semen frozen by the pellet or straw methods (56·8 and 50·8%). The fertility results suggest that the critical threshold may not have been reached at 1 × 106 frozen-thawed motile spermatozoa.

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Pregnancy rates following intra-uterine insemination with pellet or straw-frozen ram semen

Cited by 15 publications

References 7 publications

Effect of Some Alternative Components of Egg Yolk in Tris-Extender on Sperm Characteristics of Ram Semen Frozen with Two Methods of Packaging Semen

Effect of Some Alternative Components of Egg Yolk in Tris-Extender on Sperm Characteristics of Ram Semen Frozen with Two Methods of Packaging Semen

Comparison of Fertility of Estrous Synchronized Ewes with Four Different Intravaginal Devices during the Breeding Season.

Fertility of young Cashmere goats after laparoscopic insemination

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