Pregnancy-related changes in rat cervical glycosaminoglycans

Golichowski, Alan M.; King, Steven R.; Mascaro, K

doi:10.1042/bj1920001

Cited by 70 publications

(34 citation statements)

References 34 publications

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“…This includes a rise in % water and a fall in collagen concentration when expressed as a % wet weight (Table 2; Harkness, 1964;Bryant, Greenwell & Weeks, 1968;Golichowski, King & Mascara, 1980 (1980) and Cabrai, Huszar, Romero & Naftolin (1981) found no change in total glycosaminoglycan concentration (as a % of dry weight) during pregnancy although there was an alteration in the relative proportions of different glycosaminoglycans with a selective accumulation of hyaluronate. Therefore, the biochemical changes associated with softening have still to be resolved.…”

Section: Discussionmentioning

confidence: 99%

Changes in the tensile properties and fine structure of the rat cervix in late pregnancy and during parturition

Williams¹,

Hollingsworth²,

Dixon³

1982

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Section: Discussionmentioning

confidence: 99%

Changes in the tensile properties and fine structure of the rat cervix in late pregnancy and during parturition

Williams¹,

Hollingsworth²,

Dixon³

1982

Reproduction

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“…29 During pregnancy, the total collagen content of the cervix increases, whereas the concentration of collagen decreases. [29][30][31][32] As the cervix ripens, the collagen fibrils become disorganized, and more space is created between the fibrils; thus, the concentration of collagen decreases. 29,30,32 Hydroxyproline is an amino acid unique to collagen and is used to measure the collagen content of tissues.…”

mentioning

confidence: 99%

Quantitative Ultrasound Assessment of the Rat Cervix

McFarlin

O’Brien

Oelze

et al. 2006

Journal of Ultrasound in Medicine

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Objective-The purpose of this research was to detect cervical ripening with a new quantitative ultrasound technique.Methods-Cervices of 13 nonpregnant and 65 timed pregnant (days 15, 17, 19, 20, and 21 of pregnancy) Sprague Dawley rats were scanned ex vivo with a 70-MHz ultrasound transducer. Ultrasound scatterer property estimates (scatterer diameter [SD], acoustic concentration [AC], and scatterer strength factor [SSF]) from the cervices were quantified and then compared to hydroxyproline and water content. Insertion loss (attenuation) was measured in 3 rats in each of the 6 groups. Discriminant analysis was used to predict gestational age group (cervical ripening) from the ultrasound variables SD, SSF, and AC.Results-Differences were observed between the groups (SD, AC, and SSF; P < .0001). Quantitative ultrasound measures changed as the cervix ripened: (1) SD increased from days 15 to 21; (2) AC decreased from days 15 to 21; and (3) SSF was the greatest in the nonpregnant group and the least in the day 21 group. Cervix hydroxyproline content increased as pregnancy progressed (P < .003) and correlated with group, SD, AC, and SSF (P < .001). Discriminant analysis of ultrasound variables predicted 56.4% of gestational group assignment (P < .001) and increased to 77% within 2 days of the predicted analysis. Cervix insertion loss was greatest for the nonpregnant group and least for the day 21 group.Conclusions-Quantitative ultrasound predicted cervical ripening in the rat cervix, but before use in humans, quantitative ultrasound will need to predict gestational age in the later days of gestation with more precision.Keywords cervical attenuation; cervical ripening; collagen; preterm birth; quantitative ultrasound; rat cervixThe underlying mechanisms preceding labor are not yet fully understood. Preterm birth is the second leading cause of infant mortality in the United States. 1 Among the survivors, 20% to 25% have a major disability, 2 and it is estimated that just the initial neonatal care costs $6 billion annually. 3 The preterm birth rate was 12.5% in 2004, and the 16% increase in the incidence of preterm birth in the United States between 1990 and 2004 is an indication of the seriousness of this problem. , it is necessary to assume a geometric model (called a form factor) for the scatterers. These parameters were estimated by modeling the tissues as scatterers with a Gaussian form factor. The Gaussian form factor assumes that the scatterers do not have sharp discontinuities in acoustic impedance with the surrounding tissues (like glass beads in agar) but have more of a gradual change in impedance with surrounding tissues. The Gaussian form factor model was theorized to be appropriate for cervix tissue because this form factor has been shown to be a good predictor of scattering in many soft tissues. 17-21 The Gaussian form factor was chosen from several other available form factors because it gave the smallest χ 2 value relative to the scattered spectra. Furthermore, the properties of the spherical Gau...

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“…Hyaluronate is one of the primary constituents of the extracellular matrix of the human uterine cervix, and it has been reported that the mount of hyaluronate increases to about 10 times the regular level at the onset of labor and decreases quickly to the former level after parturition [7,9]. This dramatic change appears to indicate that hyaluronate plays an important role in the regulation of uterine cervical function during parturition.…”

Section: Discussionmentioning

confidence: 99%

“…In human uterine cervical connective tissue, it has been reported that the amount of hyaluronate increases markedly at the last stage of pregnancy and quickly decreases after parturition [7][8][9]. However, the mechanism responsible for the rapid depolymerization of hyaluronate after parturition in human uterine cervix has not yet been clarified.…”

Section: Introductionmentioning

confidence: 99%

Hyaluronate depolymerization activity induced by progesterone in cultured fibroblasts derived from human uterine cervix

et al. 1994

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AlmtractHigh-molecular-weight [t4C]hyaluronate was incubated with cultured fibroblasts from human uterine cervix and skin, and then the depolymerization of the hyaluronate was investigated.[14C]Hyaluronate in the medium of skin fibroblasts was depolymerized into a constant molecular weight (Mr about 40,000), whereas that of cervix fibroblasts was not depolymerized, irrespective of incubation period. However, when progesterone was added to the medium of cervix fibroblasts, hyaluronate was depolymerized to the same extent as that in skin fibroblasts. The reducing terminal sugar of the depolymerized hyaluronate was N-aeetylglucosamine. These results suggest that a hyaluronate-depolymerizing enzyme, endo-,&N-acetylglucosaminidase, was induced by progesterone in cultured fibroblasts derived from human uterine cervix.

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Pregnancy-related changes in rat cervical glycosaminoglycans

Cited by 70 publications

References 34 publications

Changes in the tensile properties and fine structure of the rat cervix in late pregnancy and during parturition

Changes in the tensile properties and fine structure of the rat cervix in late pregnancy and during parturition

Quantitative Ultrasound Assessment of the Rat Cervix

Hyaluronate depolymerization activity induced by progesterone in cultured fibroblasts derived from human uterine cervix

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