Pregnancy-Related MiRNAs Participate in the Regulation of the Immune System during the Gestational Period

Laresgoiti-Servitje, Estibalitz

doi:10.4172/2155-9899.1000361

Cited by 3 publications

(3 citation statements)

References 67 publications

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“…We further validated that 8 and 4 out of the 84 significant miRNAs were differentially expressed in milk 33 and plasma 34 , respectively, during early pregnancy in cattle, including bta-miR-20a, bta-miR-106b, bta-miR-100, bta-miR-143, bta-miR-99b, bta-miR-125b, bta-miR-125a, bta-miR-93, bta-miR-99a-5p, bta-miR-99b, bta-miR-125b, and bta-miR-29a. Our findings were consistent with previous findings that miRNAs played an important role in pregnancy, especially for fetal growth and regulation of the immune system 35 , 36 .…”

Section: Resultssupporting

confidence: 93%

Genetic and epigenetic architecture of paternal origin contribute to gestation length in cattle

Fang

Jiang

et al. 2019

Commun Biol

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The length of gestation can affect offspring health and performance. Both maternal and fetal effects contribute to gestation length; however, paternal contributions to gestation length remain elusive. Using genome-wide association study (GWAS) in 27,214 Holstein bulls with millions of gestation records, here we identify nine paternal genomic loci associated with cattle gestation length. We demonstrate that these GWAS signals are enriched in pathways relevant to embryonic development, and in differentially methylated regions between sperm samples with long and short gestation length. We reveal that gestation length shares genetic and epigenetic architecture in sperm with calving ability, body depth, and conception rate. While several candidate genes are detected in our fine-mapping analysis, we provide evidence indicating ZNF613 as a promising candidate for cattle gestation length. Collectively, our findings support that the paternal genome and epigenome can impact gestation length potentially through regulation of the embryonic development.

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Section: Resultssupporting

confidence: 93%

Genetic and epigenetic architecture of paternal origin contribute to gestation length in cattle

Fang

Jiang

et al. 2019

Commun Biol

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“…For our data analysis, we focused on placenta-specific EV-miRNAs identified a priori based on a literature search. [22][23][24][25][26] Specifically, several miRNAs from the chromosome 19 miRNA cluster (C19MC) and C14MC clusters were selected as candidates. 24…”

Section: Isolation and Profiling Of Placenta-derived Circulating Ev-mmentioning

confidence: 99%

Maternal Phthalate and Personal Care Products Exposure Alters Extracellular Placental miRNA Profile in Twin Pregnancies

et al. 2019

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Prenatal exposure to endocrine-disrupting chemicals (EDCs) exerts both short- and long-term adverse effects on the developing fetus. However, the mechanisms underlying these effects have yet to be uncovered. Maternal-fetal signaling is mediated in part by signaling molecules (eg, microRNAs [miRNAs]) contained in extracellular vesicles (EVs) that are released by the placenta into the maternal circulation. We investigated whether maternal exposure to the EDCs phthalates and personal care products alters the miRNA profile of placental-derived EVs circulating in maternal blood. Blood and urine samples from pregnant women with uncomplicated term dichorionic, diamniotic twin pregnancies were analyzed as part of a larger study investigating correlations between exposure of phthalate and personal care products and epigenetic alterations in twin pregnancies. We explored correlations between maternal urinary levels of 13 phthalate and 12 personal care products metabolites and the miRNA profile of placental EVs (EV-miRNAs) circulating in maternal blood. The expression of miR-518e was highest among women with high urinary levels of monobenzyl phthalate and methyl paraben. miR-373-3p was the least expressed in women exposed to high levels of methyl paraben, and miR-543 was significantly downregulated in women exposed to high levels of paraben metabolites, dichlorophenol metabolites, and triclosan. In conclusion, this pilot study reveals that prenatal exposure to EDCs is associated with altered profile of circulating placenta-derived EV-miRNAs. Further studies are needed to generalize these results to singleton pregnancies and to assess whether these alterations are associated with pregnancy complications.

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“…The miRNAs are non‐coding, genetically transcribed, small molecules that fine‐tune the protein regulation, post‐transcriptionally (Gottesman, 2004 ). They are among the novel regulators of gene expression in the foetal and maternal tissues not only during conception but also throughout the pregnancy (Bidarimath and Tayade, 2017 , Laresgoiti‐Servitje, 2015 ). The pregnancy‐associated miRNAs are implicated in numerous processes such as angiogenesis, trophoblast differentiation and the regulation of the maternal immune system (Laresgoiti‐Servitje, 2015 , Lycoudi et al., 2015 , Ali et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

miR‐1246 is implicated as a possible candidate for endometrium remodelling facilitating implantation in buffalo (Bubalus bubalis)

Dubey

Batra

Sarwalia

et al. 2022

Veterinary Medicine & Sci

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Background The microRNAs (miRs) secreted by the trophectoderm (TE) cells have recently been implicated in the conceptus‐endometrial cross talk during implantation and placentation. These miRs modulate various cellular processes during conception and throughout the pregnancy by regulating the gene expression in the foetal and maternal tissues. Objectives This study was undertaken to elucidate the function of TE secreted miRNAs in the maternal‐foetal cross‐talk during implantation/placentation in buffalo. Methods The in vitro produced blastocysts were cultured on a cumulus feeder layer for 21 days. The relative expression profiles of a selected panel of miRs was generated using the spent media collected on Days 0, 7, 12, 16, and 21. A custom‐designed mirVana™ miRNA mimic was used to transfect the endometrial epithelial cells (EECs) in order to determine the role of miRNA exhibiting highest expression on Days 21 and 21. Results The expression of miR‐1246 ( p < 0.001) and let‐7b ( p < 0.01) was found to be significantly higher on Day 21 of TE culture in comparison to the control (Day 0). This elevated expression indicated the involvement of these miRs in the maternal‐foetal cross‐talk. Interestingly, after the transfection of EECs with miRNA mimic for miR‐1246 (a novel molecule vis‐à‐vis implantation), the expression of beta‐catenin and mucin1 in these cells was found to be significantly ( p < 0.05) downregulated vis‐à‐vis the control, that is, the IFN‐τ primed EECs (before transfection). Conclusions The TE secreted miR‐1246 appeared to lower the expression of the endometrial receptivity genes (mucin1 and beta‐catenin) which apparently assists the endometrium in preparing for placentation.

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Pregnancy-Related MiRNAs Participate in the Regulation of the Immune System during the Gestational Period

Cited by 3 publications

References 67 publications

Genetic and epigenetic architecture of paternal origin contribute to gestation length in cattle

Genetic and epigenetic architecture of paternal origin contribute to gestation length in cattle

Maternal Phthalate and Personal Care Products Exposure Alters Extracellular Placental miRNA Profile in Twin Pregnancies

miR‐1246 is implicated as a possible candidate for endometrium remodelling facilitating implantation in buffalo (Bubalus bubalis)

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