Pregnancy-specific beta-1-glycoprotein 1-enriched exosomes are involved in the regulation of vascular endothelial cell function during pregnancy

Jia, Linyan; Huang, Xiaojie; Hao, Peng; Jia, Yuanhui; Zhang, Ruonan; Wei, Yingying; Wei, Mengtian; Liang, Han; He, Qizhi; Wang, Kai

doi:10.1016/j.placenta.2023.06.014

Cited by 2 publications

(2 citation statements)

References 36 publications

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“…PSG1 has been demonstrated in numerous studies to stimulate cell invasion, migration, and proliferation and inhibit inflammatory factors [ 38 , 39 ]. The mechanism by which PSG1 impacts the function of human trophoblast cells remains unknown.…”

Section: Discussionmentioning

confidence: 99%

Pregnancy-Specific Beta-1-Glycoprotein 1 Increases HTR-8/SVneo Cell Migration through the Orai1/Akt Signaling Pathway

Wang,

Fang,

et al. 2024

Biomolecules

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The impaired invasion ability of trophoblast cells is related to the occurrence of preeclampsia (PE). We previously found that pregnancy-specific beta-1-glycoprotein 1 (PSG1) levels were decreased in the serum of individuals with early-onset preeclampsia (EOPE). This study investigated the effect of PSG1 on Orai1-mediated store-operated calcium entry (SOCE) and the Akt signaling pathway in human trophoblast cell migration. An enzyme-linked immunosorbent assay (ELISA) was used to determine the level of PSG1 in the serum of pregnant women with EOPE. The effects of PSG1 on trophoblast proliferation and migration were examined using cell counting kit-8 (CCK8) and wound healing experiments, respectively. The expression levels of Orai1, Akt, and phosphorylated Akt (p-Akt) were determined through Western blotting. The results confirmed that the serum PSG1 levels were lower in EOPE women than in healthy pregnant women. The PSG1 treatment upregulated the protein expression of Orai1 and p-Akt. The selective inhibitor of Orai1 (MRS1845) weakened the migration-promoting effect mediated by PSG1 via suppressing the Akt signaling pathway. Our findings revealed one of the mechanisms possibly involved in EOPE pathophysiology, which was that downregulated PSG1 may reduce the Orai1/Akt signaling pathway, thereby inhibiting trophoblast migration. PSG1 may serve as a potential target for the treatment and diagnosis of EOPE.

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Section: Discussionmentioning

confidence: 99%

Pregnancy-Specific Beta-1-Glycoprotein 1 Increases HTR-8/SVneo Cell Migration through the Orai1/Akt Signaling Pathway

Wang,

Fang,

et al. 2024

Biomolecules

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“…EVs interfere with a variety of physiological and pathological regulatory processes by participating in intercellular information exchange. EVs can not only participate in physiological processes such as nervous system signaling [ 22 ], reproduction and development [ 23 ], and cell proliferation and maturation [ 24 ] but also activate the immune system by releasing proinflammatory factors and tumor necrosis factor (TNF) and enhancing NK cell activity [ 25 ], thereby participating in pathological processes such as tumor progression, inflammation, and pathogen infection and transmission.…”

Section: The Formation and Function Of Evsmentioning

confidence: 99%

Research progress on the role of extracellular vesicles in the pathogenesis of diabetic kidney disease

Jin,

Zhang

2024

Renal Failure

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Diabetic kidney disease (DKD) is a serious complication of diabetes mellitus (DM) and has become the main cause of end-stage renal disease worldwide. In recent years, with the increasing incidence of DM, the pathogenesis of DKD has received increasing attention. The pathogenesis of DKD is diverse and complex. Extracellular vesicles (EVs) contain cell-derived membrane proteins, nucleic acids (such as DNA and RNA) and other important cellular components and are involved in intercellular information and substance transmission. In recent years, an increasing number of studies have confirmed that EVs play an important role in the development of DKD. The purpose of this paper is to explain the potential diagnostic value of EVs in DKD, analyze the mechanism by which EVs participate in intercellular communication, and explore whether EVs may become drug carriers for targeted therapy to provide a reference for promoting the implementation and application of exosome therapy strategies in clinical practice.

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Pregnancy-specific beta-1-glycoprotein 1-enriched exosomes are involved in the regulation of vascular endothelial cell function during pregnancy

Cited by 2 publications

References 36 publications

Pregnancy-Specific Beta-1-Glycoprotein 1 Increases HTR-8/SVneo Cell Migration through the Orai1/Akt Signaling Pathway

Pregnancy-Specific Beta-1-Glycoprotein 1 Increases HTR-8/SVneo Cell Migration through the Orai1/Akt Signaling Pathway

Research progress on the role of extracellular vesicles in the pathogenesis of diabetic kidney disease

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