Introduction: Blood left in the umbilical cord and placenta after birth is known as umbilical cord blood stem cell. Umbilical cord blood is taken from the umbilical vein after the birth when cord is cut and clamped. Umbilical cord blood is stored in both private and public cord banks. Umbilical cord blood can be stored for up to 25-75 years. It is collected through a non-invasive procedure after the birth of the child. This procedure is safe and doesn’t harm the mother and her baby. Objectives /Aims: To assess the level of knowledge among antenatal mothers regarding umbilical cord blood stem cell banking and to find the significant association of knowledge and their demographic variables. Method: The research approach for present study is quantitative approach aimed to assess the knowledge regarding the umbilical cord blood stem cell banking among antenatal mothers. The research design used for this study is descriptive design. The study was conducted in selected hospital at Dehradun. The sample size for this study are 90 antenatal mothers. Convenient sampling technique are used for this study. Self- structured questionnaire method are used for data collection. Result: The present study finding revealed that the majority of the antenatal mothers (64.4%) had poor knowledge, (35.5%) had average knowledge and remaining (0%) had good knowledge. The study result revealed that there was statistically significant association between the level of knowledge with their demographic variables. Conclusion: The purpose of the present study was to find out the knowledge regarding the umbilical cord blood stem cell banking among antenatal mothers in selected hospital of Dehradun. Majority of the antenatal mothers had less knowledge regarding umbilical cord blood stem cell banking and there is need to improve it with the help of health education programme.