Preimplantation Endometrial Transcriptomics in Natural Conception Cycle of the Rhesus Monkey

Bhat, Muzaffer Ahmed; Khan, Meraj A.; Lalitkumar, P. G. L.; Poorasamy, Jeevitha; Sengupta, Jayasree; Ghosh, Debabrata

doi:10.3390/reprodmed3010003

Cited by 2 publications

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References 84 publications

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“…Further, there is a notion that post-ovulatory sexual activity may help facilitate a successful implantation and pregnancy, suggesting an adaptive role for this reproductive behaviour. Reports have demonstrated that semen facilitates the endometrial receptivity towards embryo growth and implantation [7][8][9] .…”

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confidence: 99%

Sperm in the implantation-stage blastocyst: Is it a check point of embryo quality?

Huppertz

Sengupta

Kroneis

et al. 2022

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The early human embryo derived from fusion of an oocyte with a single sperm undergoes growth and differentiation and results in an implantation-ready blastocyst. To initiate implantation, the blastocyst hatches from the zona pellucida, thus making it accessible to external inputs. Our modelling of sperm distribution through the uterus indicates that 200–5000 sperms following natural coitus during mid-luteal phase are in a position of reaching the implantation-stage blastocyst in the maternal uterus. We indeed have ultrastructural evidence of sperm cells intruding into the trophectoderm cells of uterine zona-free blastocysts obtained from rhesus monkeys. The question arises whether the negotiation between additional sperm and azonal blastocyst is a feature of evolution yielding adaptation processes influencing the developmental fate of an individual embryo or a neutral by-product in placental mammals. This process potentially bears implications in pregnancy success, sperm competition, and human health.

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Section: Main Textmentioning

confidence: 99%

Sperm in the implantation-stage blastocyst: Is it a check point of embryo quality?

Huppertz

Sengupta

Kroneis

et al. 2022

Preprint

Self Cite

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Overexpression of ErbB-1 (EGFR) Protein in Eutopic Endometrium of Infertile Women with Severe Ovarian Endometriosis during the ‘Implantation Window’ of Menstrual Cycle

et al. 2022

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The strong association between endometriosis and infertility is of high clinical significance. High proliferative bias in eutopic endometrium during the secretory phase is a hallmark of endometriosis, which may result in high occurrence of implantation failure and resultant infertility in endometriosis. The ErbB family of proteins regulates the proliferation capacity in the endometrium, potentially causing endometrial hostility to the implantation process in endometriosis. However, our knowledge regarding the involvement of the ErbB family in human endometrium during the window of implantation (WOI) in endometriosis-associated infertility is scant. In the present study, the cellular profiles of immunopositive ErbBs-1 to -4 in the endometrium of endometriosis-free, infertile women (Group 1; n = 11) and in eutopic endometrium of infertile women diagnosed with stage IV ovarian endometriosis (Group 2; n = 13) during the mid-secretory phase were compared using standardized guidelines. Computer-aided standardized combinative analysis of immunoprecipitation in different compartments revealed an overexpression of ErbB-1 in the epithelial, stromal and vascular compartments, along with marginally higher ErbB-3 expression (p < 0.06) in the vascular compartment and ErbB-4 expression (p < 0.05) in the glandular epithelium and stroma in the endometrium during the WOI in women with primary infertility associated with stage IV ovarian endometriosis compared with disease-free endometrium of control infertile women. It appears that changes in ErbBs in the eutopic endometrium during WOI induce anomalous proliferative, inflammatory and angiogenic activities in it, which can antagonize endometrial preparation for embryo implantation in endometriosis. This knowledge appears usable in strategizing methods for the treatment of endometriosis-associated infertility, as well as preempting the oncogenic potential of endometriosis.

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Preimplantation Endometrial Transcriptomics in Natural Conception Cycle of the Rhesus Monkey

Cited by 2 publications

References 84 publications

Sperm in the implantation-stage blastocyst: Is it a check point of embryo quality?

Sperm in the implantation-stage blastocyst: Is it a check point of embryo quality?

Overexpression of ErbB-1 (EGFR) Protein in Eutopic Endometrium of Infertile Women with Severe Ovarian Endometriosis during the ‘Implantation Window’ of Menstrual Cycle

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