Preimplantation genetic screening for aneuploidy of embryos after in vitro fertilization in women aged at least 35 years: a prospective randomized trial

Debrock, Sophie; Melotte, Cindy; Spiessens, Carl; Peeraer, Karen; Vanneste, Evelyne; Meeuwis, Luc; Meuleman, Christel; Frijns, Jean-Pierre; Vermeesch, Joris Robert; D’Hooghe, Thomas

doi:10.1016/j.fertnstert.2008.10.072

Cited by 124 publications

(76 citation statements)

References 24 publications

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“…Thus, downstream developmental processes can be irreparably compromised by removing a cell from the embryo. Such an impact is also reflected in a lower blastocyst rate after cleavage-stage biopsy with respect to undisturbed embryos, as reported in several papers [32][33][34].…”

Section: Discussionmentioning

confidence: 71%

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

Bar-El

Kalma

Malcov

et al. 2016

J Assist Reprod Genet

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Purpose The purpose of the study was to explore the effect of blastomere biopsy for preimplantation genetic diagnosis (PGD) on the embryos' dynamics, further cleavage, development, and implantation. Methods The study group included 366 embryos from all PGD treatments (September 2012 to June 2014) cultured in the EmbryoScope™ time-lapse monitoring system. The control group included all intracytoplasmic sperm injection (ICSI) embryos cultured in EmbryoScope™ until day 5 during the same time period (385 embryos). Time points of key embryonic events were analyzed with an EmbryoViewer™. Results Most (88 %) of the embryos were biopsied at ≥8 cells. These results summarize the further dynamic development of the largest cohort of biopsied embryos and demonstrate that blastomere biopsy of cleavage-stage embryos significantly delayed compaction and blastulation compared to the control non-biopsied embryos. This delay in preimplanation developmental events also affected postimplantation development as observed when the dynamics of non-implanted embryos (known implantation data (KID) negative) were compared to those of implanted embryos (KID positive). Conclusion Analysis of morphokinetic parameters enabled us to explore how blastomere biopsy interferes with the dynamic sequence of developmental events. Our results show that biopsy delays the compaction and the blastulation of the embryos, leading to a decrease in implantation.

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Section: Discussionmentioning

confidence: 71%

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

Bar-El

Kalma

Malcov

et al. 2016

J Assist Reprod Genet

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“…It took above noted Dutch study [2] to stop to a degree this worldwide utilization of PGS. Other studies later reaffirmed the results reported by Mastenbroek et al [3][4][5][6]. Professional organizations followed by unequivocally concluding that PGS, at current practice levels, did not improve clinical IVF outcomes [7][8][9].…”

Section: Introductionmentioning

confidence: 72%

“…In such patients a day-3 embryo transfer may result in a viable pregnancy, while the same marginal embryo, cultured to days-5/6, may not survive prolonged culture. As a consequence, PGS#2, like PGS#1 before [2][3][4][5][6] in DOR patients may, therefore, actually reduce pregnancy chances.…”

Section: The Misdirection Of Ongoing Studies Of Pgs#2mentioning

confidence: 99%

A review of, and commentary on, the ongoing second clinical introduction of preimplantation genetic screening (PGS) to routine IVF practice

Gleicher

Barad

2012

J Assist Reprod Genet

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“…Contrary to expectation, results from numerous studies did not show that the PGS-FISH was capable of improving live birth rates in patients of advanced maternal age, with recurrent implantation failure or repeated pregnancy loss [35][36][37][38][39][40][41][42][43][44][45][46]. This is because FISH screening does not make it possible to test for all 23 chromosome pairs.…”

Section: Time Lapse Monitoringmentioning

confidence: 83%

Selecting a Single Embryo for Transfer after In-vitro Fertilization: A Translational Medicine Perspective

Faduola¹

2015

Transl Biomed

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Preimplantation genetic screening for aneuploidy of embryos after in vitro fertilization in women aged at least 35 years: a prospective randomized trial

Cited by 124 publications

References 24 publications

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

A review of, and commentary on, the ongoing second clinical introduction of preimplantation genetic screening (PGS) to routine IVF practice

Selecting a Single Embryo for Transfer after In-vitro Fertilization: A Translational Medicine Perspective

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