Preimplantation genetic screening in women of advanced maternal age caused a decrease in clinical pregnancy rate: a randomized controlled trial

Hardarson, Thorir; Hanson, Charles; Lundin, Kersti; Hillensjö, T.; Nilsson, Lars; Stevic, Justina; Reismer, E.; Borg, Kia; Wikland, Matts; Bergh, Christina

doi:10.1093/humrep/den217

Cited by 219 publications

(162 citation statements)

References 31 publications

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“…Thus, downstream developmental processes can be irreparably compromised by removing a cell from the embryo. Such an impact is also reflected in a lower blastocyst rate after cleavage-stage biopsy with respect to undisturbed embryos, as reported in several papers [32][33][34].…”

Section: Discussionmentioning

confidence: 71%

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

Bar-El

Kalma

Malcov

et al. 2016

J Assist Reprod Genet

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Purpose The purpose of the study was to explore the effect of blastomere biopsy for preimplantation genetic diagnosis (PGD) on the embryos' dynamics, further cleavage, development, and implantation. Methods The study group included 366 embryos from all PGD treatments (September 2012 to June 2014) cultured in the EmbryoScope™ time-lapse monitoring system. The control group included all intracytoplasmic sperm injection (ICSI) embryos cultured in EmbryoScope™ until day 5 during the same time period (385 embryos). Time points of key embryonic events were analyzed with an EmbryoViewer™. Results Most (88 %) of the embryos were biopsied at ≥8 cells. These results summarize the further dynamic development of the largest cohort of biopsied embryos and demonstrate that blastomere biopsy of cleavage-stage embryos significantly delayed compaction and blastulation compared to the control non-biopsied embryos. This delay in preimplanation developmental events also affected postimplantation development as observed when the dynamics of non-implanted embryos (known implantation data (KID) negative) were compared to those of implanted embryos (KID positive). Conclusion Analysis of morphokinetic parameters enabled us to explore how blastomere biopsy interferes with the dynamic sequence of developmental events. Our results show that biopsy delays the compaction and the blastulation of the embryos, leading to a decrease in implantation.

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Section: Discussionmentioning

confidence: 71%

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

Bar-El

Kalma

Malcov

et al. 2016

J Assist Reprod Genet

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“…It took above noted Dutch study [2] to stop to a degree this worldwide utilization of PGS. Other studies later reaffirmed the results reported by Mastenbroek et al [3][4][5][6]. Professional organizations followed by unequivocally concluding that PGS, at current practice levels, did not improve clinical IVF outcomes [7][8][9].…”

Section: Introductionmentioning

confidence: 72%

“…The failure of PGS#1, however, refuted their prediction in its universality. As noted before, studies demonstrated not only lack of efficacy in improving IVF outcomes but, actually, decreased pregnancy chances as consequence of PGS in older women [1][2][3]. The theoretical concept that pretransfer elimination of aneuploid embryos should improve IVF outcomes has, however, nevertheless survived, though remains in search of a suitable patient population where PGS can, indeed, be unequivocally demonstrated to be effective in improving IVF outcomes.…”

Section: Search Strategymentioning

confidence: 95%

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A review of, and commentary on, the ongoing second clinical introduction of preimplantation genetic screening (PGS) to routine IVF practice

Gleicher

Barad

2012

J Assist Reprod Genet

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“…93% of trisomy 18, 95% of trisomy 21 and 100% of trisomy 16. Advanced maternal age is the major risk factor for oocyte aneuploidy, mainly due to premature separation of sister chromatids [4,5].…”

Section: Introductionmentioning

confidence: 99%

Are zona pellucida laser drilling and polar body biopsy safe for in vitro matured oocytes?

Hammoud

Molina-Gomès

Albert

et al. 2010

J Assist Reprod Genet

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Introduction Preconception diagnosis requires first polar body biopsy. When the hole in the zona pellucida is made with a laser beam, heat propagation could, like the biopsy itself, be deleterious. Our aim was to evaluate the effect of this technique on human in vitro matured oocyte and embryo development. Methods One hunded fifty five retrieved immature oocytes from 75 women, matured in vitro, were distributed in 3 groups: 50 oocytes in a control group, without laser drilling and first polar body biopsy, 52 oocytes in a group with only laser drilling, and 53 oocytes in a group with both laser drilling and first polar body biopsy. Safety was evaluated using four criteria: [1] oocyte lysis rate, [2] oocyte activation rate, [3] oocyte development after calcium ionophore treatment, [4] and embryo chromosome breakage incidence after Tarkowski preparation. Results No difference in the four criteria was observed between the 3 oocyte groups.Conclusions We did not find evidence of deleterious effect of laser drilling and first polar body biopsy on in vitro matured oocytes, according to our criteria.

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Preimplantation genetic screening in women of advanced maternal age caused a decrease in clinical pregnancy rate: a randomized controlled trial

Abstract: ISRCTN38014610.

Cited by 219 publications

References 31 publications

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

A review of, and commentary on, the ongoing second clinical introduction of preimplantation genetic screening (PGS) to routine IVF practice

Are zona pellucida laser drilling and polar body biopsy safe for in vitro matured oocytes?

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