Preincubation With bFGF but Not Sodium Ascorbate Improves Efficiency of Autologous Transplantation of Muscle-derived Cells Into Urethral Wall

Burdzińska, Anna; Bartoszuk, Urszula; Orzechowski, Arkadiusz

doi:10.1016/j.urology.2008.11.024

Cited by 10 publications

(8 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It should be emphasized that it is still not known whether autologous cell transfer itself is able to induce inflammation that can contribute to graft elimination; however, it is generally accepted that autotransplantation does not cause an inflammatory response. Surprisingly, a beneficial effect of preconditioning with antioxidant was not observed when MDC were autotransplanted into the urethral wall 36. Possible causes for this difference in observed reactions include the distinct site of the host tissue (skeletal muscle vs. urethral wall), the presence of repair processes at the injection site vs. no injury, and/or a different method of evaluation of cell survival.…”

Section: Discussionmentioning

confidence: 97%

Protective effect of sodium ascorbate on efficacy of intramuscular transplantation of autologous muscle‐derived cells

Bartoszuk-Bruzzone¹,

Burdzińska²,

Orzechowski³

et al. 2011

Muscle and Nerve

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 97%

Protective effect of sodium ascorbate on efficacy of intramuscular transplantation of autologous muscle‐derived cells

Bartoszuk-Bruzzone¹,

Burdzińska²,

Orzechowski³

et al. 2011

Muscle and Nerve

Self Cite

View full text Add to dashboard Cite

show abstract

“…17 The obtained population was resuspended in the growth medium, which consisted of Dulbecco Modified Eagle's Medium (DMEM) supplemented with 10% (v/v) fetal bovine serum, 5%…”

Section: Isolation and Culture Of Porcine Muscle-derived Cellsmentioning

confidence: 99%

The Effect of Endoscopic Administration of Autologous Porcine Muscle-derived Cells Into the Urethral Sphincter

Burdzińska

Crayton

Dybowski

et al. 2013

Urology

Self Cite

View full text Add to dashboard Cite

“…Currently, the available therapeutic approaches to improve the urethral rhabdosphincter function are limited. The transplantation of muscle derived cells (MDC) into the urethral wall was previously tested in small animal models (Yiou et al, ; Chermansky et al, ; Burdzinska et al, ). Promising outcomes have made it possible to start clinical trials.…”

mentioning

confidence: 99%

The Anatomy of Caprine Female Urethra and Characteristics of Muscle and Bone Marrow Derived Caprine Cells for Autologous Cell Therapy Testing

Burdzińska

Dybowski

Zarychta-Wiśniewska

et al. 2016

The Anatomical Record

View full text Add to dashboard Cite

Cell therapy is emerging as an alternative treatment of stress urinary incontinence. However, many aspects of the procedure require further optimization. A large animal model is needed to reliably test cell delivery methods. In this study, we aim to determine suitability of the goat as an experimental animal for testing intraurethral autologous cell transplantation in terms of urethral anatomy and cell culture parameters. The experiments were performed in 12 mature/aged female goats. Isolated caprine muscle derived cells (MDC) were myogenic in vitro and mesenchymal stem cells (MSC) population was able to differentiate into adipo-, osteo- and chondrogenic lineages. The median yield of cells after 3 weeks of culture amounted 47 × 10(6) for MDC and 37 × 10(6) for MSC. Urethral pressure profile measurements revealed the mean functional urethral length of 3.75 ± 0.7 cm. The mean maximal urethral closure pressure amounted 63.5 ± 5.9 cmH O and the mean functional area was 123.3 ± 19.4 cm*cmH O. The omega- shaped striated urethral sphincter was well developed in the middle and distal third of the urethra and its mean thickness on cross section was 2.3 mm. In the proximal part of the urethra only loosely arranged smooth muscle fibers were identified. To conclude, presented data demonstrate that caprine MDC and MSC can be expanded in vitro in a repeatable manner even when mature or aged animals are cell donors. Results suggest that female caprine urethra has similar parameters to those reported in human and therefore the goat can be an appropriate experimental animal for testing intraurethral cell transplantation. Anat Rec, 00:000-000, 2016. © 2016 Wiley Periodicals, Inc. Anat Rec, 300:577-588, 2017. © 2016 Wiley Periodicals, Inc.

show abstract

Preincubation With bFGF but Not Sodium Ascorbate Improves Efficiency of Autologous Transplantation of Muscle-derived Cells Into Urethral Wall

Cited by 10 publications

References 26 publications

Protective effect of sodium ascorbate on efficacy of intramuscular transplantation of autologous muscle‐derived cells

Protective effect of sodium ascorbate on efficacy of intramuscular transplantation of autologous muscle‐derived cells

The Effect of Endoscopic Administration of Autologous Porcine Muscle-derived Cells Into the Urethral Sphincter

The Anatomy of Caprine Female Urethra and Characteristics of Muscle and Bone Marrow Derived Caprine Cells for Autologous Cell Therapy Testing

Contact Info

Product

Resources

About