Preinvasive and invasive cervical cancer: an ex vivo proton magic angle spinning magnetic resonance spectroscopy study

Abstract

The aim of this study was to obtain (1)H MR spectra using magic angle spinning (MAS) techniques from punch biopsies (<20 mg) of preinvasive and invasive cervical disease and to correlate the spectral profiles with sample classification on the basis of histopathology. Tissue samples were obtained at colposcopic examination, during local treatment of cervical intraepithelial neoplasia (CIN) or at hysterectomy. (1)H MAS MRS was performed at 25 degrees C while spinning the sample at 4.5 kHz. After measurement, the tissue was immersed in formalin and the pathology determined. Histological examination after (1)H MAS MRS defined 27 samples with squamous cell carcinoma (SCC), 12 with CIN and 39 with only normal tissue. The standardized integrals of the lipid, choline and creatine regions of the spectra were significantly higher in SCC than in normal or CIN tissue. There was no obvious difference in the standardized integral of the region 4.15-3.5 ppm. The acyl fatty acid side-chain length was longer or less unsaturated in SCC than in normal tissue. Normal tissue from patients with SCC showed significantly higher triglycerides than normal tissue from patients with benign uterine disease but significantly lower triglycerides than SCC tissue. (1)H MAS MRS of the uterine cervix ex vivo may be used to differentiate non-invasive from invasive cervical lesions, increase interpretation of in vivo MRS and provide insights into tumor biology.

Can nuclear magnetic resonance (NMR) spectroscopy reveal different metabolic signatures for lung tumours?

“…In particular, it proved to be very powerful for corroborating and complementing noninvasive in vivo magnetic resonance spectroscopy studies by aiding assignment and quantification of the in vivo data, generally characterised by poor resolution and signal-tonoise at clinical magnetic field strengths [10][11][12][13][14]. Moreover, much research work has focused on the metabolic differentiation between tumour and healthy tissues, aiming to find possible biomarkers for the presence and/or grade of different cancers such as breast [15,16], brain [17,18], prostate [19][20][21], cervical [22][23][24], colorectal [25,26], hepatic [27], renal [28,29] and gastric cancer [30].…”

Detection of cancer in cervical tissue biopsies using mobile lipid resonances measured with diffusion-weighted 1 H magnetic resonance spectroscopy

“…MLR have been shown to be involved in tumour cell physiology, growth and metastasis (5) and cellular studies, which measure various lipid components in defined conditions, have demonstrated a correlation between an increase in MLR with suppressed proliferation (6)(7)(8), ongoing apoptosis (9)(10)(11)(12), growth arrest (7,12), and necrosis (13,14). In tissue studies, metabolic profiling of cervical biopsies (15)(16)(17) and other cancers (18,19) with magnetic resonance spectroscopy has been previously described, but measurement of MLR peaks has been confounded by signals from low molecular weight metabolites which complicate peak assignment and quantification. Several methods have been used to improve the discrimination of lipid signals from those of low molecular weight metabolites.…”

Detection of Metabolite Changes in C6 Glioma Cells Cultured with Antimitotic Oleyl Glycoside by 1H MAS NMR

“…[8][9][10] In this regard, proton MAS NMR combined with T 2 -filtering has been used to narrow the resonances from small molecules and improve the resolution of intact cells and tissues spectra, allowing weak resonances associated with metabolites present at low concentrations to be revealed. [11][12][13][14][15] The nondestructive nature of the technique allows the acquisition of metabolic information directly from cells with minimal preparation and their analysis by other methods. In addition, the solid state approach offers the advantage over conventional liquid state NMR (i.e., used in analysis of cells extracts) of simultaneous observation of lipophilic and hydrophilic metabolites.…”