Preliminary analysis of recombinant β-tubulin of Pseudocohnilembus persalinus (Ciliophora: Scuticociliatida) as a vaccine antigen candidate against scuticociliatosis

Kim, Sung Mi; Lee, Eun Hye; Kwon, Se Ryun; Lee, Sun Joung; Kim, Sung Koo; Nam, Yoon Kwon; Kim, Ki Hong

doi:10.1016/j.aquaculture.2006.06.016

Cited by 6 publications

(2 citation statements)

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“…By immune proteomic screening, tubulin has been determined as one of the candidates for vaccine development against C. irritans ( 19 ). In addition, recombinant β-tubulin has been suggested as a target antigen to control scuticociliatosis, which is caused by parasitic ciliates ( 22 ). I-antigens are surface membrane proteins present on ciliates, and they are considered as the primary vaccine candidates against parasitic ciliates ( 23 – 25 ).…”

Section: Discussionmentioning

confidence: 99%

“…However, as the protective immunity induced by I-antigens is serotype specific ( 26 , 27 ), vaccination based on I-antigens may not cover different C. irritans serotypes existing in nature. Tubulin is a well-conserved protein in ciliates, and vaccines using this molecule may solve the problem raised by different serotypes ( 22 ). As expected, grouper immunized with rTt-tubulin showed induced protective immunity, but the protective rate was lower than that of T. thermophila –immunized grouper, which may due to other antigens existing in T. thermophila that may provide immunity stimulation.…”

Section: Discussionmentioning

confidence: 99%

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Protection of Grouper Against Cryptocaryon irritans by Immunization With Tetrahymena thermophila and Protective Cross-Reactive Antigen Identification

et al. 2022

Front. Immunol.

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Vaccination is an effective method to prevent Cryptocaryon irritans infection. Although some vaccines have been developed, large-scale production of these vaccines is costly. Development of a heterogenous vaccine generated by low-cost antigens is an alternative method. In the present study, grouper immunized with Tetrahymena thermophila, a free-living ciliate that easily grows in inexpensive culture media at high density, showed protective immunity against C. irritans infection. Higher immobilization against C. irritans theronts was detected in T. thermophila–immunized grouper serum, which suggested the existence of a cross-reactive antibody in the serum. By immunoprecipitation and mass spectrometry analyses, tubulin was identified as a potential cross-reactive antigen between C. irritans and T. thermophila. Recombinant T. thermophila tubulin protein (rTt-tubulin) and its antibody were prepared, and immunofluorescence showed that both C. irritans and T. thermophila cilia were stained by the anti–rTt-tubulin antibody. Grouper immunized with rTt-tubulin showed a reduced infective rate after the C. irritans challenge. An enhanced level of C. irritans–binding immunoglobulin M (IgM) antibody was detected in serum from rTt-tubulin–immunized grouper. Moreover, specific antibodies were also found in the mucus and tissue culture medium from rTt-tubulin–immunized grouper. Overall, these findings suggested that vaccination with T. thermophila elicits cross-reactive protective immunity in grouper against C. irritans, and T. thermophila may be a potential heterologous antigen for vaccine development.

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