Preliminary analysis of the antimicrobial activity of a postoperative wound dressing containing chlorhexidine gluconate against methicillin-resistant Staphylococcus aureus in an in vivo porcine incisional wound model

Mana, Thriveen Sankar Chittoor; Donskey, Curtis J.; Carty, Neal; Perry, Larry; Leaper, David; Edmiston, Charles E.

doi:10.1016/j.ajic.2019.05.012

Cited by 10 publications

(6 citation statements)

References 34 publications

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“…Furthermore, alginate and other polymeric hydrogels-based wound dressings have also shown effectiveness in managing surgical incisions [ 316 , 317 ]. In another study, chlorhexidine gluconate (CHG) film-based antimicrobial wound dressings have shown effectiveness with strong antimicrobial activity and wound healing properties in post-operative wounds in the porcine incisional wound model [ 318 ].…”

Section: In Vivo Methods For Evaluation Of Wound Dressing Materialsmentioning

confidence: 99%

In Vitro and In Vivo Characterization Methods for Evaluation of Modern Wound Dressings

Ahmad

2022

Pharmaceutics

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Chronic wound management represents a major challenge in the healthcare sector owing to its delayed wound-healing process progression and huge financial burden. In this regard, wound dressings provide an appropriate platform for facilitating wound healing for several decades. However, adherent traditional wound dressings do not provide effective wound healing for highly exudating chronic wounds and need the development of newer and innovative wound dressings to facilitate accelerated wound healing. In addition, these dressings need frequent changing, resulting in more pain and discomfort. In order to overcome these issues, a wide range of affordable and innovative modern wound dressings have been developed and explored recently to accelerate and improve the wound healing process. However, a comprehensive understanding of various in vitro and in vivo characterization methods being utilized for the evaluation of different modern wound dressings is lacking. In this context, an overview of modern dressings and their complete in vitro and in vivo characterization methods for wound healing assessment is provided in this review. Herein, various emerging modern wound dressings with advantages and challenges have also been reviewed. Furthermore, different in vitro wound healing assays and in vivo wound models being utilized for the evaluation of wound healing progression and wound healing rate using wound dressings are discussed in detail. Finally, a summary of modern wound dressings with challenges and the future outlook is highlighted.

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Section: In Vivo Methods For Evaluation Of Wound Dressing Materialsmentioning

confidence: 99%

In Vitro and In Vivo Characterization Methods for Evaluation of Modern Wound Dressings

Ahmad

2022

Pharmaceutics

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“…Regarding CHG, its efficacy in reducing MRSA contamination in surgical wounds has been previously tested in a porcine model, demonstrating significant antimicrobial activity and emphasizing the potential of CHG dressings against MRSA [37]. Furthermore, meta-analyses support this by stating that dressings impregnated with CHG significantly reduce infections related to central venous catheters, with rare adverse effects [38,39].…”

Section: Discussionmentioning

confidence: 95%

Wound Dressing Impregnated with Vancomycin and Chlorhexidine Digluconate in the Prevention of Orthopedic Surgical Site Infections: A Pilot Study Using a Low-Cost Procedu

Campelo,

Maziero,

Manfrinato

et al. 2023

Preprint

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This study introduces a novel approach by proposing a dressing impregnated with vancomycin and chlorhexidine for postoperative care, specifically targeting patients undergoing orthopedic surgeries. The research design involved a pilot study with a randomized control group, aiming to evaluate the vancomycin with chlorhexidine dressing's efficacy and safety. A total of 12 patients used impregnated dressing and 9 used the same dressing without impregnation. Despite limitations, including the small sample size and single-center study location, our findings demonstrate the safety of the impregnated dressing in trauma surgeries, indicating potential applicability in broader surgical contexts. In conclusion, this study contributes to the discourse on preventive strategies for SSIs, presenting a pioneering approach with the vancomycin and chlorhexidine-impregnated dressing. Future research endeavors, incorporating larger-scale studies and addressing study limitations, are crucial for advancing the understanding and implementation of effective postoperative care strategies.

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“…CD and bacitracin are widely used as wound dressings and in topical treatment. However, they fail to completely decontaminate S. aureus -infected wounds [ 36 ], in addition to their associated cytotoxicity that might interfere with the healing process [ 37 ]. We showed that combination of non-antibiotic agents including antiseptics with bacitracin successfully upgraded their capacity to kill planktonic S. aureus (although the results are unpublished).…”

Section: Discussionmentioning

confidence: 99%

A Three-Dimensional Model of Bacterial Biofilms and Its Use in Antimicrobial Susceptibility Testing

Ali,

Collier,

Bayston

2024

Microorganisms

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(1) Background: The discrepant antimicrobial susceptibility between planktonic and biofilm bacterial modes poses a problem for clinical microbiology laboratories and necessitates a relevant 3D experimental model allowing bacteria to grow in biofilm mode, in vitro, for use in anti-biofilm susceptibility testing. (2) Methods: This work develops a 3D biofilm model consisting of alginate beads containing S. aureus biofilm and encased within two thick layers of alginate matrix. The constructed model was placed on a thin Boyden chamber insert suspended on a 24-well culture plate containing the culture medium. The antibacterial activity of bacitracin and chlorhexidine digluconate (CD), either combined or separately, against 2D S. aureus culture was compared to that in the 3D biofilm model. Quantitative analysis and imaging analysis were performed by assessing the bacterial load within the matrix as well as measuring the optical density of the culture medium nourishing the matrix. (3) Results: The 3D biofilm model represented the typical complex characteristics of biofilm with greater insusceptibility to the tested antimicrobials than the 2D culture. Only bacitracin and CD in combination at 100× the concentration found to be successful against 2D culture were able to completely eliminate the 3D biofilm matrix. (4) Conclusions: The 3D biofilm model, designed to be more clinically relevant, exhibits higher antimicrobial insusceptibility than the 2D culture, demonstrating that the model might be useful for testing and discovering new antimicrobial therapies. The data also support the view that combination therapy might be the optimal approach to combat biofilm infections.

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Preliminary analysis of the antimicrobial activity of a postoperative wound dressing containing chlorhexidine gluconate against methicillin-resistant Staphylococcus aureus in an in vivo porcine incisional wound model

Cited by 10 publications

References 34 publications

In Vitro and In Vivo Characterization Methods for Evaluation of Modern Wound Dressings

In Vitro and In Vivo Characterization Methods for Evaluation of Modern Wound Dressings

Wound Dressing Impregnated with Vancomycin and Chlorhexidine Digluconate in the Prevention of Orthopedic Surgical Site Infections: A Pilot Study Using a Low-Cost Procedu

A Three-Dimensional Model of Bacterial Biofilms and Its Use in Antimicrobial Susceptibility Testing

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