2006
DOI: 10.1590/s1516-93322006000100005
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Preliminary approach to detect amylolytic and pectinolytic activities from maca (Lepidium meyenii Walp.)

Abstract: Amylase (AM), pectinesterase (PE) and polygalacturonase (PG) were extracted from maca roots (Lepidium meyenii Walp). The response surface model was used to determine the optimum conditions of pH and temperature activity of each enzyme on the crude extract. The highest values of AM activity occurred at pH 6.1 and 33.6 °C, very close to the experimental central point. The PE activity had a maximum activity at pH 6.6 and 49.4 and PG activity showed optimum activity at pH 5.4 and 46 °C.

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Cited by 2 publications
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“…For example, glucosinolates are hydrolyzed by endogenous myrosinases into glucose, 6) 7) and structural carbohydrates are hydrolyzed into low molecular weight sugars by endogenous amylase, pectinesterase, and polygalacturonase. 26) Consequently, reducing sugars will react with the amino groups of proteins. Although there is a lack of evidence for the presence of enzymes responsible for enzymatic browning (i.e.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…For example, glucosinolates are hydrolyzed by endogenous myrosinases into glucose, 6) 7) and structural carbohydrates are hydrolyzed into low molecular weight sugars by endogenous amylase, pectinesterase, and polygalacturonase. 26) Consequently, reducing sugars will react with the amino groups of proteins. Although there is a lack of evidence for the presence of enzymes responsible for enzymatic browning (i.e.…”
Section: Resultsmentioning
confidence: 99%
“… 25) 30) 31) 32) In a comparison of the ∆ H gel values between the freeze-dried maca and commercial one, we found that the ∆ H gel of commercial maca was much lower than that of freeze-dried maca ( Table 1 ). Partial gelatinization and enzymatic hydrolysis induced by endogenous amylases 26) occur during air-drying and thermal sterilization. This interpretation is supported by the fact that commercial maca had a much higher OD 420 than freeze-dried maca samples.…”
Section: Resultsmentioning
confidence: 99%