Preliminary assessment of methanogenic microbial communities in marine caves of Zakynthos Island (Ionian Sea, Greece)

Abstract

Mediterranean marine caves remain largely unexplored, while particularly limited information is available about microbial life in these unique environments. This study is a preliminary assessment of the composition of the active anaerobic microbial community colonizing the walls of newly explored systems of underwater caves and small cavities in Zakynthos Island. The interior of these caves is densely coated with egg-shaped, foam-shaped and filamentous biological structures that are characterised by a strong odour of hydrogen sulphide gas. A total of twelve structures scrapped from cave rocks were subjected to anaerobic cultivation for up to 208 days. Strong to moderate methanogenesis was observed in two different types of egg-shaped structures and one foamlike structure. Interestingly, this was observed in experiments that were performed at room temperature (i.e. 25 o C), which is substantially lower than those typically considered optimum for methane production (e.g. 35 o C). An analysis of the 16S rRNA genes revealed a clear dominance of archaea and bacteria closely related to known methane producers and sulphate reducers, including members of the families Methanomicrobiaceae, Desulfobulbaceae, Desulfobacteraceae, Desulfuromonaceae, Campylobacteraceae, Marinifilaceae, Clostridiaceae, Incertae Sedis -Family I & II. These results show that Mediterranean marine caves can host members of archaea and bacteria with potential biotechnological interest that deserves further investigation.

The Essentials of Marine Biotechnology

“…The organisms hosted in these environments are of particular biotechnological interest. A range of mesophilic and thermotolerant microorganisms have been reported from submarine caves and cavities, characterized by elevated concentrations of hydrogen sulfide (Canganella et al, 2006), while methanogenic and sulfate reducing microbial species with potential applications in biogas production and bioremediation have been isolated from similar environments (Polymenakou et al, 2018). Besides biogas production, the biotechnological interest of bacteria originating from unique submarine caves could be even greater in terms of their secondary metabolites.…”