Preliminary Assessment of Photoactivated Antimicrobial Collagen on Bioburden in a Murine Pressure Ulcer Model

Lanzafame, Raymond J.; Stadler, István; Cunningham, R. S.; Muhlbauer, Aaron; Griggs, Jacob; Soltz, Robert; Soltz, Barbara A.

doi:10.1089/pho.2012.3423

Cited by 13 publications

(4 citation statements)

References 27 publications

(35 reference statements)

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“…11 It is important to emphasize that bacterial colonization of ulcers can interfere with the healing process. In an early experiment, Lanzafame et al 12 inoculated methicillin-resistant S. aureus in a murine pressure ulcer model (Balb/C mice) and observed no positive results following blue-laser irradiation at 455 -5 nm (350 mW, 1 cm diameter, for 15 min). There are scant studies demonstrating the effect of blue laser on bacterial control, and among the existing studies, few used this light spectrum in different bacterial species.…”

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confidence: 99%

Blue Laser Inhibits Bacterial Growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa

Sousa

Santos

Gomes

et al. 2015

Photomedicine and Laser Surgery

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Objective: The purpose of this study was to analyze the influence of blue laser on bacterial growth of the main species that usually colonize cutaneous ulcers, as well as its effect over time following irradiation. Background data: The use of blue laser has been described as an adjuvant therapeutic method to inhibit bacterial growth, but there is no consensus about the best parameters to be used. Methods: Strains of Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, and Escherichia coli ATCC 25922 were suspended in saline solution at a concentration of 1.5 · 10 3 colony forming units (CFU)/mL. Next, 300 lL of this suspension was transferred to a microtitulation plate and exposed to a single blue laser irradiation (450 nm) at fluences of 0 (control), 3, 6, 12, 18, and 24 J/cm 2 . Each suspension was spread over the surface of a Petri plate before being incubated at 37°C, and counts of CFU were determined after 24 and 48 h. Results: Blue laser inhibited the growth of S. aureus and P. aeruginosa at fluences > 6 J/cm 2 . On the other hand, E. coli was inhibited at all fluences tested, except at 24 J/cm 2 . Conclusions: Blue laser light was capable of inhibiting bacterial growth at low fluences over time, thus presenting no time-dependent effect.

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confidence: 99%

Blue Laser Inhibits Bacterial Growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa

Sousa

Santos

Gomes

et al. 2015

Photomedicine and Laser Surgery

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“…First, the study has employed a murine skin wound ischemia reperfusion model of pressure injury. Although this model has been validated in multiple publications, 12 – 15 , 37 – 39 the translatability of findings in a murine skin model to the human condition faces some challenges. Rodent skin healing occurs in part due to contraction rather than reepithelialization.…”

Section: Discussionmentioning

confidence: 99%

A Novel, Sterilized Microvascular Tissue Product Improves Healing in a Murine Pressure Ulcer Model

Gimble

Frazier

et al. 2018

Plastic and Reconstructive Surgery - Global Open

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Background:Processed microvascular tissue (PMVT), a human structural allograft, is derived from lyophilized human tissue containing microcirculatory cellular components. Since PMVT serves as a source of extracellular matrix (ECM), growth factors, cytokines, and chemokines modulating angiogenesis, inflammation, apoptosis, and endogenous cell recruitment, we hypothesized its application would accelerate wound regeneration in a validated pressure ulcer (PU) model developed in C57BL/6 mice using two 24-hour cycles of skin ischemia/reperfusion created by placement and removal of external magnets.Methods:Two identical PU injuries (n = 50 female mice) were treated with (a) topical particulate PMVT, (b) injected rehydrated PMVT, or (c) saline control injection, and assessed daily for closure rates, scab formation/removal, and temperature. A baseline control cohort (n = 5) was euthanized at day 0 and treatment group cohorts (n = 5) were killed at 3, 7, or 14 days postinjury. The PU injuries were collagenase-digested for flow cytometric analysis of inflammatory, reparative, and stem cell frequencies and analyzed by hematoxylin and eosin (H&E) histology and immunofluorescence.Results:PMVT-accelerated wound closure, most notably, topical PMVT significantly increased mean closure from d5 (13% versus -9%) through d13 (92% versus 38%) compared with phosphate-buffered saline (PBS) controls (P < 0.05). PMVT also hastened scab formation/removal, significantly accelerated disappearance of inflammatory myeloid (CD11b+) cells while upregulating α-smooth muscle actin, vascular endothelial growth factor A, and placental growth factor and raised skin temperature surrounding the PU site, consistent with increased blood flow.Conclusions:These results indicate that PMVT has potential as an advanced treatment for restoring normal tissue function in ischemic wounds and merits clinical study.

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“…Because of the highest expression level of FSP-1 in M-MDSCs among all MDSC subpopulations, to test our hypothesis, we used FSP-1-Cre/KLF4(flox) mice to produce PUs [72]. The dorsal skin of WT and FSP-1-Cre/KLF4(flox) (KLF4 null) mice were shaved, gently pulled up and placed between two cylinders of magnets (12 mm in diameter and 5 mm in thickness), producing a compressive pressure of 50 mmHg between the two magnets according to the established PU model [72][73][74]. A single ischemiareperfusion cycle (I/R) consisted of a period of magnet placement for 16 h followed by a release or rest of 8 h. Three I/R cycles were used in each animal to initiate decubitus ulcer formation.…”

Section: Klf4 Deficiency Compromised Cutaneous Wound Healing Dependin...mentioning

confidence: 99%

KLF4-Mediated Plasticity of Myeloid-Derived Suppressor Cells (MDSCs)

Fan¹,

Raychoudhury²,

Ai³

2020

Cells of the Immune System

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Robustness of tissues refers to their capability to maintain normal functions despite perturbation such as injuries. Recent studies suggest a key role of the immune system in injury repair. In this process, several immune cell lineages exhibit considerable plasticity as they migrate toward the site of damage and contribute to repair. For example, myeloid-derived suppressor cells (MDSCs) are a heterogeneous group of immature cells and possess phenotypic plasticity in cancer, a pathological status that is considered as "wounds that do not heal." They are characterized by their potent ability to suppress immune responses. In cutaneous wound healing, MDSCs not only execute their immunosuppressive function to inhibit inflammation but also stimulate cell proliferation once they adopt a fate of a totally different cell type. At a molecular level, we found that Krüppel-like factor 4 (KLF4), a transcription factor with multiple roles in homeostasis and disease development plays a critical role in regulating MDSCs. In this review, KLF4-mediated plasticity of MDSCs and the underlying mechanisms are discussed.

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Preliminary Assessment of Photoactivated Antimicrobial Collagen on Bioburden in a Murine Pressure Ulcer Model

Cited by 13 publications

References 27 publications

Blue Laser Inhibits Bacterial Growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa

Blue Laser Inhibits Bacterial Growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa

A Novel, Sterilized Microvascular Tissue Product Improves Healing in a Murine Pressure Ulcer Model

KLF4-Mediated Plasticity of Myeloid-Derived Suppressor Cells (MDSCs)

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