Preliminary characterisation of a lipolytic activity from an extremely halophilic archaeon, Natronococcus sp.

Boutaiba, Saad; Bhatnagar, Tej; Hacène, Hocine; Mitchell, David A.; Baratti, J.

doi:10.1016/j.molcatb.2006.03.010

Cited by 78 publications

(52 citation statements)

References 35 publications

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“…It contains an extracellular lipase that was optimally active at 4 M NaCl, pH 7, and 50°C, and it was more active against the substrate p-nitrophenyl palmitate (C16). The enzyme hydrolyzed olive oil, indicating the presence of a true lipase, being the first one reported in archaea [71]. After that, a total of 118 halophilic archaeal strains were screened for lipolytic activity.…”

Section: Esterases and Lipasesmentioning

confidence: 99%

Biocompounds from Haloarchaea and Their Uses in Biotechnology

Torregrosa-Crespo¹,

Galiana²,

MaríaMartínez-Espinosa³

2017

Archaea - New Biocatalysts, Novel Pharmaceuticals and Various Biotechnological Applications

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New advances in the understanding of haloarchaea physiology, metabolism, biochemistry, and molecular biology show that these kinds of microorganisms produce several compounds in response to the extreme conditions of their ecosystems. Thus, the complete metabolic and genetic machineries are fully adapted to nutrient starvation, high sun radiation, and high ionic strength. Due to these adaptations, some of the primary and secondary metabolites produced by haloarchaea are of high interest in terms of potential biotechnological uses. The principal goal of the chapter is to present a review about the main characteristics of these biocompounds and their potential uses in biomedicine, pharmacy, and industry.

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Section: Esterases and Lipasesmentioning

confidence: 99%

Biocompounds from Haloarchaea and Their Uses in Biotechnology

Torregrosa-Crespo¹,

Galiana²,

MaríaMartínez-Espinosa³

2017

Archaea - New Biocatalysts, Novel Pharmaceuticals and Various Biotechnological Applications

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“…Many lipases lose their activity under these conditions and, therefore, efforts are being made to obtain lipases that remain active [234]. It has been proposed that esterases and lipases from extremophiles are suitable candidates for applications in the above-mentioned harsh reaction conditions [235].…”

Section: Lipases and Esterasesmentioning

confidence: 99%

“…strain TC6 produced a thermostable lipase with optimum activity at 4 M NaCl, 50 C and pH 7, without any activity in the absence of salt. The enzyme showed thermal stability and retained more than 90 % of its original activity after incubation at 50 C for 60 min [234].…”

Section: Lipases and Esterasesmentioning

confidence: 99%

Systematics of haloarchaea and biotechnological potential of their hydrolytic enzymes

et al. 2017

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Halophilic archaea, also referred to as haloarchaea, dominate hypersaline environments. To survive under such extreme conditions, haloarchaea and their enzymes have evolved to function optimally in environments with high salt concentrations and, sometimes, with extreme pH and temperatures. These features make haloarchaea attractive sources of a wide variety of biotechnological products, such as hydrolytic enzymes, with numerous potential applications in biotechnology. The unique trait of haloarchaeal enzymes, haloenzymes, to sustain activity under hypersaline conditions has extended the range of already-available biocatalysts and industrial processes in which high salt concentrations inhibit the activity of regular enzymes. In addition to their halostable properties, haloenzymes can also withstand other conditions such as extreme pH and temperature. In spite of these benefits, the industrial potential of these natural catalysts remains largely unexplored, with only a few characterized extracellular hydrolases. Because of the applied impact of haloarchaea and their specific ability to live in the presence of high salt concentrations, studies on their systematics have intensified in recent years, identifying many new genera and species. This review summarizes the current status of the haloarchaeal genera and species, and discusses the properties of haloenzymes and their potential industrial applications. SYSTEMATICS OF HALOPHILIC ARCHAEAHalophilic micro-organisms are found in the three domains of life, and are extremophiles living in hypersaline environments in the presence of salt (generally NaCl) as a specific requisite for their growth [1]. Hypersaline environments contain higher salt concentrations than seawater (3.5 % total dissolved salts) [2]. According to the optimum NaCl concentration for growth, micro-organisms fall into one of the following categories: extreme halophiles with optimum growth at 15-30 % (2.5-5.2 M) NaCl; moderate halophiles with optimum growth at 3-15 % (0.5-2.5 M) NaCl; slight halophiles with optimum growth at 1-3 % (0.2-0.5 M) NaCl; non-halophiles with optimum growth at less than 1 % (0.2 M) NaCl; and halotolerant micro-organisms which are non-halophiles showing the ability to tolerate high NaCl concentrations [3]. Halophiles can live in hypersaline environments because they are able to sustain osmotic balance. They use two main strategies to withstand the adverse consequences of water loss when exposed to high salt concentrations. The first strategy used by extremely halophilic archaea (haloarchaea) and halophilic anaerobic bacteria such as members of Halanaerobiales is known as the 'salt-in-cytoplasm' strategy, during which salts such as NaCl or KCl accumulate in the cytoplasm at concentrations similar to the extracellular ones [4,5]. Another mechanism to cope with high salt concentrations is the accumulation of organic molecules such as amino acids, sugars and polyols, known as compatible solutes or osmolytes. Compatible solutes are small and highly soluble molecules that can...

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“…They look to be the very good aspirant for industrial application, besides existence salt-loving, they may consume excellent activity, at high temperature, low water activity and high pH. Present day, only a few reports have occurred on the characterization of halophilic archaea isolated from Algerian saline ecosystem that studies absorbed mainly on saline lakes [24][25][26][27].…”

Section: Enzyme Productionmentioning

confidence: 99%

An Overview of Saltpan Halophilic Bacterium

Manikandan¹,

Pk²

2017

J Antimicrob Agents

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Hypersaline environments provide an excellent medium for natural microbial communities which serve as a potential source of pharmaceutical substances. Salt is widely present in the earth. Almost 73% of earth was covered with marine water which contains 2.5% of common salt. Protease enzyme activity widespread in microorganisms, plant and animals. Proteolytic enzymes used in the industrial application and bioremediation process. In recent years' new mutant's microbe resistant to commonly used antibiotics. Protease inhibitors used as potential antibiotics for controlling microbial infections. A Hypersaline environment such as salt pans and salt lakes has high salt concentration and pH. The saltpan provides a diversity of different environmental conditions of alkalinity, salinity, temperature, pH and nutrition. Halophilic organisms growing between 0.5 and 3.0 M salt concentration. Extreme environments are the best source of bioactive compound producing halophiles microbes.

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Preliminary characterisation of a lipolytic activity from an extremely halophilic archaeon, Natronococcus sp.

Cited by 78 publications

References 35 publications

Biocompounds from Haloarchaea and Their Uses in Biotechnology

Biocompounds from Haloarchaea and Their Uses in Biotechnology

Systematics of haloarchaea and biotechnological potential of their hydrolytic enzymes

An Overview of Saltpan Halophilic Bacterium

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