Preliminary Characterization of Dog Derived Pathogenic Strains of Leptospira interrogans Serovar Australis in Nanchang of Jiangxi Province, China

Song, Ning; Zhang, Wenlong; Ding, Yue; Wu, Di; Dai, Zonghao; Xu, Lingling; Cao, Yongguo

doi:10.3389/fvets.2020.607115

Cited by 6 publications

(4 citation statements)

References 19 publications

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“…There are only a few epidemiological reports of canine leptospirosis in China, most based on surveys of cities in the south [ 22 , 23 ], which is consistent with the fact that leptospirosis occurs mainly in warm and humid parts of the south [ 24 , 25 ]. Changchun is located in the subtropical region of China.…”

Section: Introductionmentioning

confidence: 78%

“…In our study, the other three serogroups, L. Australis, L. Ballum and L. Pyrogenes, accounted for more than half of the positive samples and should be concerned. Although pathogenic L. Pomona and L. Pyrogenes have not been reported in China, our team successfully isolated the L. Australis strain of Leptospirosis from an infected dog in 2020 [24]. These three types of Leptospira are likely to become potential pathogens and threaten dogs' health.…”

Section: Discussionmentioning

confidence: 99%

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Seroepidemiological Analysis of Canine Leptospira Species Infections in Changchun, China

et al. 2023

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Leptospirosis is a significant worldwide zoonotic infectious disease that infects a wide range of animals and humans. Leptospira will colonize the animal’s urinary and reproductive systems and be excreted with urine, potentially causing a wide range of infections. Dogs are an essential host for Leptospira, and epidemiological investigation studies of leptospirosis must be conducted to clarify the prevalence of leptospirosis and to reduce the risk of transmission to humans. This study aimed to investigate the seroepidemiology of leptospiral infection in dogs from Changchun, China, using Microscopic Agglutination Test (MAT). A total of 1053 canine blood samples were collected and tested by MAT. The positive rate of MAT was approximately 19.1%. The main prevalent Leptospira serogroups were L. Icterohaemorrhagiae (8.1%), L. Canicola (7.6%), L. Australis (5.3%), L. Ballum (4.7%) and L. Pyrogenes (4.2%). No statistically significant difference among different varieties, sexes and sampling seasons (p > 0.05), except the age (p < 0.05). The seropositive rate was much higher in adult and aged dogs than in juvenile dogs. Our results showed the seroprevalence and the prevalent serogroup of Canine leptospirosis in Changchun, China.

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Section: Introductionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Seroepidemiological Analysis of Canine Leptospira Species Infections in Changchun, China

et al. 2023

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“…Nonetheless, Leptospira spp. are important zoonotic pathogens with approximately 500,000 globally reported cases annually, with high lethality depending on species, strain, and case severity (Esson et al., 2019; Song et al., 2021). Exposure to Leptospira has been demonstrated in humans with unexplained sepsis in Cambodia and in people with undifferentiated febrile illness in Northern Thailand (Rozo et al., 2020; Takhampunya et al., 2019).…”

Section: Discussionmentioning

confidence: 99%

A multipronged next‐generation sequencing metabarcoding approach unearths hyperdiverse and abundant dog pathogen communities in Cambodia

Huggins

Colella

Koehler

et al. 2021

Transbounding Emerging Dis

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Recent surveys in Southeast Asia, including Cambodia, have identified canine vector‐borne pathogens (VBPs), including those with zoonotic potential, as highly prevalent. The lack of veterinary care alongside the close association semidomesticated dogs have with humans in the region exacerbates these zoonotic risks. Nonetheless, the number of studies investigating such pathogens and the threats they pose to dog and human health is limited. Here, we utilize a next‐generation sequencing (NGS)‐based metabarcoding protocol to conduct an assumption‐free characterization of the bacterial, apicomplexan, and kinetoplastid blood‐borne pathogens of free‐roaming dogs from across Cambodia. From 467 dogs at five field sites, 62% were infected with one of eight confirmed pathogens, comprising Anaplasma platys (32%), Ehrlichia canis (20%), Hepatozoon canis (18%), Babesia vogeli (14%), Mycoplasma haemocanis (13%), the zoonotic pathogen Bartonella clarridgeiae (3%), Candidatus Mycoplasma haematoparvum (0.2%), and Trypanosoma evansi (0.2%). Coinfections of between two and four VBPs were common with 28% of dogs found to have a mixed infection. Moreover, DNA from putatively infectious agents belonging to the bacterial family and genera Coxiella, Mycobacterium, Neisseria, Rickettsiaceae, Treponema, and two uncharacterized Mycoplasma species were identified, in addition to protozoan genera Colpodella, Parabodo, and Bodo. Using a multiple logistic regression model, the presence of ectoparasites, abnormal mucous membranes, anemia, and total protein were found as predictors of canine VBP exposure. This study represents the first time an NGS metabarcoding technique has been used to holistically detect the bacterial and protozoan hemoparasites communities of dogs through an in‐depth survey, highlighting the power of such methods to unearth a wide spectrum of pathogenic organisms in an unbiased manner.

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“…The disease is caused by pathogenic spirochetes in the genus Leptospira , which currently contains 68 species, in which pathogenic groups can be divided into over 24 pathogenic serogroups and 250 pathogenic serovars based on the surface epitopes of the lipopolysaccharide (LPS) antigens on their outer membrane [ 2 , 5 , 6 , 7 ]. As various pathogenic serovars can infect humans and animals, it is crucial to identify the serovar involved and develop improved serological diagnostic techniques to understand their epidemiology and prevent the spread of disease [ 5 , 8 , 9 , 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Improved Antibody Detection for Canine Leptospirosis: ELISAs Modified Using Local Leptospiral Serovar Isolates from Asymptomatic Dogs

Boonciew,

Saisongkorh,

Brameld

et al. 2024

Animals

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Leptospirosis is a zoonotic disease of significant concern for human and animal health, with domestic animals, including dogs, acting as reservoirs for human infection. Serology is widely used for leptospirosis diagnosis, even though the standard microscopic agglutination test (MAT) using a panel of serovars lacks specificity and can lead to detection limitations in certain regions. In this study, we aimed to develop an antibody detection tool for dogs using an indirect enzyme-linked immunosorbent assay (ELISA) with a set of local serovar isolates, including Paidjan, Dadas, and Mini, to enhance the accuracy of leptospirosis surveillance in our region. The specificity and sensitivity of various antigen preparations, namely leptospiral whole-cell protein (WCP), total membrane protein (TMP), and outer membrane protein (OMP), were assessed using sera from infected and non-infected dogs, as well as negative puppy sera. Leptospirosis diagnosis was supported using a genus-specific nested polymerase chain reaction test on all collected sera. Protein preparations were validated using SDS-PAGE and Western blotting analysis. In the results, the standard MAT failed to detect antibodies in any of the dogs confirmed as being infected using PCR and isolation, highlighting its limitations. In contrast, the OMP-based ELISAs using local isolates of Leptospira serovars gave positive results with sera from all infected dogs, and negative results with sera from all dogs from non-endemic areas. IgG titres of infected and unvaccinated dogs from endemically affected areas were significantly higher than those in non-endemic regions. Using the OMP-based IgG/ELISAs with the local serovar Dadas resulted in higher specificity and lower sensitivity than when using the WCP- and TMP-based IgG/ELISAs. Agreement analysis revealed fair and moderate concordance between OMP-based IgG/ELISAs and PCR results, whereas slight and fair agreement was observed between OMP-based ELISAs and the MAT. Overall, the modified OMP-based IgG/ELISAs, utilising relevant local serovar isolates from dogs, demonstrated improved accuracy in detecting leptospirosis in the study area, overcoming the limitations of the MAT. This study highlights the importance of identifying and incorporating these local circulating serovar isolates into serological techniques for leptospirosis diagnosis and surveillance.

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Preliminary Characterization of Dog Derived Pathogenic Strains of Leptospira interrogans Serovar Australis in Nanchang of Jiangxi Province, China

Cited by 6 publications

References 19 publications

Seroepidemiological Analysis of Canine Leptospira Species Infections in Changchun, China

Seroepidemiological Analysis of Canine Leptospira Species Infections in Changchun, China

A multipronged next‐generation sequencing metabarcoding approach unearths hyperdiverse and abundant dog pathogen communities in Cambodia

Improved Antibody Detection for Canine Leptospirosis: ELISAs Modified Using Local Leptospiral Serovar Isolates from Asymptomatic Dogs

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