Preliminary evaluation of the positively and negatively charge effects of tetra-substituted porphyrins on photoinactivation of rapidly growing mycobacteria

Guterres, Kevim Bordignon; Rossi, Grazielle Guidolin; Menezes, Lucas B.; Campos, Marli Matiko Anraku de; Iglesias, Bernardo A.

doi:10.1016/j.tube.2019.06.001

Cited by 27 publications

(18 citation statements)

References 42 publications

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“…In another study, antiviral photodynamic activity was observed for porphyrin-derivative PSs [ 134 ]. Guterres et al [ 135 ] reported that cationic porphyrin derivatives showed higher singlet oxygen production with higher photostability than anionic porphyrin derivatives. Moreover, low concentrations of cationic porphyrin derivatives showed very strong antimicrobial photodynamic activity against MRSA.…”

Section: Synthetic Derivatives Of Natural Pssmentioning

confidence: 99%

Natural Photosensitizers in Antimicrobial Photodynamic Therapy

2021

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Health problems and reduced treatment effectiveness due to antimicrobial resistance have become important global problems and are important factors that negatively affect life expectancy. Antimicrobial photodynamic therapy (APDT) is constantly evolving and can minimize this antimicrobial resistance problem. Reactive oxygen species produced when nontoxic photosensitizers are exposed to light are the main functional components of APDT responsible for microbial destruction; therefore, APDT has a broad spectrum of target pathogens, such as bacteria, fungi, and viruses. Various photosensitizers, including natural extracts, compounds, and their synthetic derivatives, are being investigated. The main limitations, such as weak antimicrobial activity against Gram-negative bacteria, solubility, specificity, and cost, encourage the exploration of new photosensitizer candidates. Many additional methods, such as cell surface engineering, cotreatment with membrane-damaging agents, nanotechnology, computational simulation, and sonodynamic therapy, are also being investigated to develop novel APDT methods with improved properties. In this review, we summarize APDT research, focusing on natural photosensitizers used in in vitro and in vivo experimental models. In addition, we describe the limitations observed for natural photosensitizers and the methods developed to counter those limitations with emerging technologies.

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Section: Synthetic Derivatives Of Natural Pssmentioning

confidence: 99%

Natural Photosensitizers in Antimicrobial Photodynamic Therapy

2021

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“…Experiments in vitro have shown that Mycobacteroides abscessus subsp. Massiliense and Mycolicibacterium fortuitum were 100 times more susceptible to white-light illumination when cationic porphyrins were used (Guterres et al 2019). However, the bactericidal action of the porphyrin derivatives containing the Pt (II) complexes Massiliense and Mycolicibacterium smegmatis strains in the MIC and 2xMIC concentrations in the first 48 h after white-light illumination.…”

Section: Porphyrin Derivativesmentioning

confidence: 99%

“…In general, anionic porphyrins have a considerably lower PDI effect on mycobacteria (Guterres et al 2019). These findings were confirmed in another study (Feese and Ghiladi 2009), which evaluated the lethal effect of various photosensitisers [5,10,15,20- This work demonstrated that, in contrast to the use of anionic PS, using cationic photosensitisers in the nanomolar concentration range resulted in a 5-6 log of CFU reduction, which approximately corresponds to the destruction of 99.99% viable cells of M. smegmatis (Feese and Ghiladi 2009).…”

Section: Porphyrin Derivativesmentioning

confidence: 99%

“…In general, anionic porphyrins have a considerably lower PDI effect on mycobacteria (Guterres et al 2019 ). These findings were confirmed in another study (Feese and Ghiladi 2009 ), which evaluated the lethal effect of various photosensitisers [5,10,15,20-tetrakis (1-methyl-4-pyridinyl) porphyrin tetratosylate (TMPyP); 5,10,15,20-tetrakis (4-N,N,N-trimethylanilinium) porphyrin tetra-chloride (TNMAP); 5,10,15,20-tetrakis (4-sulphonatophenyl) porphyrin (TSPP); 5,10,15,20-tetrakis (4-carboxyphenyl) porphyrin-Pd (II)(TCPP-Pd) at different doses on M. smegmatis in vitro.…”

Section: Exogenous Photosensitisers For Antimycobacterial Pdimentioning

confidence: 99%

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Photoinactivation of mycobacteria to combat infection diseases: current state and perspectives

Shleeva

Savitsky

Kaprelyants

2021

Appl Microbiol Biotechnol

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The spread of multi-drug-resistant bacterial strains causing serious infectious diseases dictates the development of new approaches to combat these diseases. In addition to drug resistance, the important causative agent of tuberculosis (Mycobacterium tuberculosis (Mtb)) is able to persist asymptomatically in individuals for many years, causing latent forms of tuberculosis. In such a dormant state, Mtb cells are also resistant to known antibiotics. In this regard, photodynamic inactivation (PDI) could be an effective alternative to antibiotics as its action is based on the generation of active forms of oxygen independently on the presence of specific antibiotic targets, thereby inactivating both drug-resistant and dormant bacteria. In this review, we summarise examples of the application of PDI for the elimination of representatives of the genus Mycobacteria, both in vitro and in vivo. According to published results, including photosensitisers in the PDI regime results in a significantly higher lethal effect. Such experiments were mainly performed using chemically synthesised photosensitisers, which need to be transported to the areas of bacterial infections, limiting PDI usage by surface (skin) diseases. In this regard, endogenous photosensitisers (mainly porphyrins) could be used to solve the problem of transportation. In vitro experiments demonstrate the effective application of PDI for mycobacteria, including Mtb, using endogenous porphyrins; the intracellular contents of these substances can be elevated by administration of 5-aminolevulenic acid, a precursor of porphyrin synthesis. Photodynamic inactivation can also be used for dormant mycobacteria, which are characterised by high levels of endogenous porphyrins. Thus, PDI can effectively eliminate drug-resistant mycobacteria. The exploitation of modern light-transmitting techniques opens new possibilities to use PDI in clinical settings. Key points•The potential effects of photodynamic inactivation of mycobacteria are critically reviewed.•Approaches to photoinactivation of mycobacteria using exogenous and endogenous photosensitisers are described.•Prospects for the use of photodynamic inactivation in the treatment of tuberculosis are discussed.

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“…2,3 While PDT offers a promising avenue for novel drug development in governing highlyselective methods of treatment, limitations in application due to the inherent hydrophobicity and poor solubility of porphyrins and other organic photosensitizers has been noted. [4][5][6] To overcome solubility issues, zinc insertion has been shown to increase the lipophilicity of compounds, resulting in reduced aggregation, increased vesicle and cell uptake, more effective membrane binding and photooxidation, as well as higher specicity in target site delivery. 7 Glycosylation of various porphyrin systems has also proved to be an effective strategy in expanding both water-solubility and specicity in organic photosensitizers, 8 and has been shown to facilitate carbohydrate-protein interactions in cellular recognition.…”

mentioning

confidence: 99%