Preliminary Evaluation of the Wound Healing Effect of &lt;em&gt;Vitex Doniana&lt;/em&gt; Sweet (Verbenaceae) in Mice

Amegbor, kossi; Metowogo, Koffi; Eklu-Gadégbéku, Kwashie; Agbonon, Amégnona; Aklikokou, Kodjo; Napo‐Koura, Gado; Gbéassor, Messanvi

doi:10.4314/ajtcam.v9i4.16

Cited by 15 publications

(14 citation statements)

References 22 publications

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“…Mice were weighed and assessed for grooming and alertness, and images of the wound were obtained daily to measure wound contraction using ImageJ (NIH). Wound contraction was calculated as a percentage of wound area reduction using the following formula: WCd ϭ (1 Ϫ WAd/WA0) ϫ 100, where WCd is the wound contraction on day d, WAd is the wound area on day d, and WA0 is the wound area on the initial day, as previously described (47).…”

Section: Methodsmentioning

confidence: 99%

Neomycin Sulfate Improves the Antimicrobial Activity of Mupirocin-Based Antibacterial Ointments

Blanchard

Brooks

Beckley

et al. 2016

Antimicrob Agents Chemother

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In the midst of the current antimicrobial pipeline void, alternative approaches are needed to reduce the incidence of infection and decrease reliance on last-resort antibiotics for the therapeutic intervention of bacterial pathogens. In that regard, mupirocin ointment-based decolonization and wound maintenance practices have proven effective in reducing Staphylococcus aureus transmission and mitigating invasive disease. However, the emergence of mupirocin-resistant strains has compromised the agent's efficacy, necessitating new strategies for the prevention of staphylococcal infections. Herein, we set out to improve the performance of mupirocin-based ointments. A screen of a Food and Drug Administration (FDA)-approved drug library revealed that the antibiotic neomycin sulfate potentiates the antimicrobial activity of mupirocin, whereas other library antibiotics did not. Preliminary mechanism of action studies indicate that neomycin's potentiating activity may be mediated by inhibition of the organism's RNase P function, an enzyme that is believed to participate in the tRNA processing pathway immediately upstream of the primary target of mupirocin. The improved antimicrobial activity of neomycin and mupirocin was maintained in ointment formulations and reduced S. aureus bacterial burden in murine models of nasal colonization and wound site infections. Combination therapy improved upon the effects of either agent alone and was effective in the treatment of contemporary methicillin-susceptible, methicillin-resistant, and high-level mupirocin-resistant S. aureus strains. From these perspectives, combination mupirocin-and-neomycin ointments appear to be superior to that of mupirocin alone and warrant further development.

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Section: Methodsmentioning

confidence: 99%

Neomycin Sulfate Improves the Antimicrobial Activity of Mupirocin-Based Antibacterial Ointments

Blanchard

Brooks

Beckley

et al. 2016

Antimicrob Agents Chemother

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“…Leaves and fruits are sold on local and regional markets (Mapongmetsem et al, 2012a). Young leaves (eaten as spinach) and barks are used in the treatment of several human diseases (Okafor, 1991;Sanogo et al, 2009;Amegbor et al, 2012;Ouattara et al, 2013;KranjacBerisavljevic and Gandaa, 2013;Adetoro et al, 2013;Ochieng et al, 2013) as well as animal diseases (Suleiman and Yusuf, 2008;Tijjani et al, 2012;Njidda, 2012). In addition, there is a great variability in the fruit organoleptic and morphological characteristics according to the local people of the Northern Cameroon.…”

Section: Introductionmentioning

confidence: 99%

VEGETATIVE PROPAGATION Of VITEX DONIANA SWEET fROM ROOT SEGMENT CuTTINGS

Mapongmetsem¹,

Guidawa²,

Noubissie-Tchiagam³

et al. 2015

Bois for. trop.

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B o i s e t f o r ê t s d e s t r o p i q u e s , 2 0 1 6 , n° 3 2 7 ( PROPAGATION VÉGÉTATIVE DE VITEX DONIANA SWEET À PARTIR DE SECTIONS DE RACINEVitex doniana Sweet est une espèce à usages multiples d'une grande importance socio-économique et commune en Afrique tropicale. Malgré sa forte utilisation en milieu rural, elle est encore présente à l'état sauvage. Il existe peu de données sur sa domestication. La propagation de cet arbre par bouturage de segments de racine constitue une alternative à sa difficile régénéra-tion sexuée. L'objectif de notre travail était d'évaluer deux des facteurs clés (substrat d'enracinement, diamètre des boutures) qui influencent l'aptitude des boutures de segments de racine (BSR) à néoformer des pousses feuillées et des racines. Le système racinaire de 23 arbres a été partiellement excavé sur une profondeur de 20 cm. Des BSR de 15 cm de long ont été disposées horizontalement dans des polypropagateurs sur cinq substrats différents, arrosés matin et soir. Le dispositif était un split-plot à trois ré-pétitions. Le traitement principal recouvrait cinq substrats : terre noire (Tn), sable fin (S), sciure de bois (Sc), 50 % Tn/50 % Sc (TnSc) et 50 % Tn/50 % S (Tn-S). Le traitement secondaire distinguait deux classes de diamètre (0,5-1cm ; 1,1-2,5 cm). Après la mise en culture des BSR, le temps de latence d'émer-gence était de 8 semaines pour les pousses aériennes et 12 pour les racines. Après 28 semaines, le pourcentage de pousses feuillées formées variait de 28 % (Tn) à 55 % (S). Les pousses aériennes se sont développées majoritairement (82 %) sur le pôle distal. Le diamètre des BSR a déterminé le déve-loppement des pousses feuillées (P < 0,01). Le taux de bourgeonnement des BSR oscillait entre 21,0 ± 1,8 % pour les BSR de 0,5-1 cm et 86,0 ± 7,8 % pour la classe 1,1-2,5 cm. Le diamètre a également impacté l'enracinement des BSR (P < 0,001). Le taux d'enracinement des BSR variait de 12,0 ± 2,3 % pour les petits diamètres à 59,3 ± 4,7 % pour les plus gros. La multiplication végétative par BSR peut améliorer la filière économique de V. doniana dans les hautes savanes guinéennes du Cameroun. Mots-clés :Vitex doniana, multiplication végétative, domestication, aptitude à l'enracinement, bouturage de segments de racine, Cameroun. ABSTrACT VEGETATIVE PROPAGATION Of VITEX DONIANA SWEET fROM ROOT SEGMENT CuTTINGSVitex doniana Sweet is a common multipurpose species in tropical Africa, which has considerable socio-economic importance. Unfortunately, it is extracted from the wild and there has been little or no focused effort to domesticate and cultivate the species. Mastering its propagation through root segment cuttings (RSC) is a real alternative to the difficult process of regeneration from seeds. The aim of our study was to assess two of the key factors (rooting medium and root diameter) that influence its rooting ability. The root system of 23 trees was partially excavated to a depth of 20 cm. Root segment cuttings 15 cm in length were carefully cut and arranged horizontally in a polypropagato...

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“…The gel was prepared as follows: 0.3 g of Carbopol 974P NF (Goodrich, USA) was dispersed in 27 g of distilled water and mixed by stirring continuously on a magnetic stirrer (IKA-Combimag RCT) at 800 rpm for 1 h. The mixture under continuous stirring was neutralized by drop-wise addition of NaOH 1 mol/l. Mixing was continued until a transparent gel was formed [6,7]. Three types of gel formulations were prepared: empty gel, gel containing 2.5% and 5% of A. nilotica pods extract.…”

Section: Preparation Of Gelsmentioning

confidence: 99%

“…Later on, wound area was evaluated by using Image J program. Wound contraction (WC) was calculated as a percentage of the reduction in wound area by using the mathematic expression: WCd= (1-WAd/WA0) X 100 where WAd is the area of the wound on the day d and WA0 the wound area on day 0 [7,9].…”

Section: Measurement Of Wound Areamentioning

confidence: 99%

Effect of Topical Application of Hydro-Ethanolic Extract of Acacia nilotica Pods on Second-Degree Cutaneous Infected Burns

Metowogo

Darré

Daubeuf

et al. 2015

J. Pharm. Nutr. Sci.

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Acacia nilotica is a medicinal plant used traditionally in Togo to treat various diseases including burn wounds. The aim of this study was to evaluate experimentally Acacia nilotica burn wound healing effect. Cutaneous burn wounds were symmetrically caused on depilated areas of rat skin through contact with an aluminum bar (r = 10 mm), preheated at 80°C for 30 sec. Five groups of animal were constituted and each group contained 8 mice. Four groups of animal's burn wounds were infected by Staphylococcus aureus. One group burn wound is uninfected and serves as negative control. Burn wounds were assessed by planimetry and histological parameters of healing. Twelve days after burn wound induction, wound contraction in the uninfected groups (negative control) was 19.9% for topical application against-2.43% for infected control. In the infected groups treated with extract, wound contraction was generally stimulates. Histological examination showed granulated tissue developing over the wounds treated with the extract of A. nilotica at 2.5% and 5% where the proliferation of fibroblasts and neo-blood vessels was very marked. In conclusion, A. nilotica pod contents tannin, flavonoids, alkaloids and protein reduces DPPH solution and significantly accelerates wound healing of burns, and this is the case even if wounds are infected with 10 9 CFU/mL of S. aureus.

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Preliminary Evaluation of the Wound Healing Effect of <em>Vitex Doniana</em> Sweet (Verbenaceae) in Mice

Cited by 15 publications

References 22 publications

Neomycin Sulfate Improves the Antimicrobial Activity of Mupirocin-Based Antibacterial Ointments

Neomycin Sulfate Improves the Antimicrobial Activity of Mupirocin-Based Antibacterial Ointments

VEGETATIVE PROPAGATION Of VITEX DONIANA SWEET fROM ROOT SEGMENT CuTTINGS

Effect of Topical Application of Hydro-Ethanolic Extract of Acacia nilotica Pods on Second-Degree Cutaneous Infected Burns

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