Preliminary examination of factors affecting Agrobacterium tumefaciens-mediated transformation of marula, Sclerocarya birrea subsp. caffra (Anacardiacease)

Mollel, Margaret; Goyvaerts, Elisabeth

doi:10.1007/s11240-004-5364-z

Cited by 7 publications

(4 citation statements)

References 34 publications

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“…During large scale GUS assays, we found a large proportion of chimeras in both genotypes (9 out of 12 samples of 'F39' and 9 out of 13 samples of '150'). Chimeric shoots exhibited blue sectors of various sizes following GUS histochemical staining as reported in other crop species (Moore 1995;Mollel et al 2004;Kathiravan et al 2006). Most chimeric shoots did not survive kanamycin selection and died before or during rooting.…”

Section: Gus Staining Pcr and Southern Blot Analysissupporting

confidence: 62%

Agrobacterium-mediated transformation and shoot regeneration in elite breeding lines of western shipper cantaloupe and honeydew melons (Cucumis melo L.)

Ren

Bang

Curtis

et al. 2011

Plant Cell Tiss Organ Cult

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Section: Gus Staining Pcr and Southern Blot Analysissupporting

confidence: 62%

Agrobacterium-mediated transformation and shoot regeneration in elite breeding lines of western shipper cantaloupe and honeydew melons (Cucumis melo L.)

Ren

Bang

Curtis

et al. 2011

Plant Cell Tiss Organ Cult

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“…The immense potential of the marula plant is underpinned by its diverse nutritional properties, including high vitamin C levels (2.0 mg per gram of fresh juice), high protein content (60%), and high quality stable oil (56% of the kernel). The long regeneration cycle and the heterozygous nature of the plant make conventional breeding slow and difficult (Mollel and Goyvaerts 2004); hence research into its microculture will generate knowledge that will form the basis for genetic transformation research for plant improvement. The objectives of this study were to evaluate the effects of different aromatic cytokinins and auxins on the induction of shoots and roots, respectively, in the tissue culture of S. birrea, a known recalcitrant woody plant.…”

Section: Introductionmentioning

confidence: 99%

Recalcitrant effects associated with the development of basal callus-like tissue on caulogenesis and rhizogenesis in Sclerocarya birrea

2011

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In the micropropagation of woody plant species, adventitious root and shoot formation remain some of the major bottlenecks due to their recalcitrance to in vitro manipulation. Some plant growth regulators may ameliorate these recalcitrant effects and improve in vitro caulogenic and rhizogenic processes. Shoot induction on shoot meristems, hypocotyls and epicotyls was evaluated using equimolar concentrations of benzyladenine (BA), meta-topolin (mT), meta-topolin riboside (mTR), and meta-methoxytopolin riboside (MemTR). Three auxins, indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and a-naphthalene acetic acid (NAA) were used in the induction of adventitious roots. Moderately high shoot formation (62.7%) was achieved at a concentration of 8.0 lM mT after 8 weeks in culture. The highest number of adventitious shoots per explant (2.4 ± 0.3) and the longest shoots (23.5 ± 3.16 mm) were recorded on 8.0 lM mT, though not significantly different from BA treatments. Most shoots progressively produced brown basal callus, which is a potential sink for cytokinin conjugates that are inhibitory to further proliferation of adventitious shoots. Good adventitious shoot formation occurred in 55% of hypocotyl explants on 8.0 lM mT. The highest rooting (91.6%) was achieved with IBA-treated shoots at a concentration of 4.0 lM. The use of mT and IBA provide an efficient micropropagation method for S. birrea, though further research is required especially in overcoming ex vitro plantlet survival challenges.

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“…Owing to its economic potential, S. birrea has been earmarked for crop development and improvement in southern Africa (Mollel and Goyvaerts, 2004). As industrial demand for its products is increasing, there is a growing concern about the sustainable supply (Nwonwu, 2006) and conservation of wild populations.…”

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confidence: 99%

After-ripening, Light Conditions, and Cold Stratification Influence Germination of Marula [Sclerocarya birrea (A. Rich.) Hochst. subsp. caffra (Sond.) Kokwaro] Seeds

Moyo¹,

Kulkarni²,

Finnie³

et al. 2009

horts

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Marula [Sclerocarya birrea (A. Rich.) Hochst. subsp. caffra (Sond.) Kokwaro (Anacardiaceae)] is used in many African countries as a food crop and is also in demand for industrial purposes. The fruit pulp has high vitamin C levels and the nuts have a high protein and oil content. The fruit pulp is commercially used in the production of an alcoholic beverage (Amarula Cream) and the oil is gaining importance in the cosmetic industry. Although attempts are being made to domesticate this high-value indigenous tree, there is very limited information available on aspects of seed germination. Our study investigated the role of light, temperature, cold stratification, and after-ripening on seed germination of S. birrea. Temperatures between 25 and 35 °C favored germination of opercula-removed seeds under continuous dark conditions. White light completely inhibited seed germination with the inhibitory effect being reversed when seeds were transferred to dark conditions. This photoinhibitory effect on opercula-removed seeds was lost after 12 months of seed storage at room temperature in the dark. Cold stratification (5 °C) of intact seeds for 14 days significantly improved germination (65%) as compared with nonstratified seeds (32%). Pregermination treatments (acid scarification, boiling water, dry heat, soaking, and plant growth regulators) of S. birrea seeds did not promote germination. Seeds of S. birrea can be considered orthodox because they tolerated desiccation without significant loss of viability. Both intact and opercula-removed seeds readily imbibe water suggesting physiological rather than physical dormancy. The highest germination percentage was recorded under constant dark conditions at 25 °C for opercula-removed seeds exposed to an after-ripening period of 12 months. This study indicates that after-ripening, light conditions, and cold stratification are critical factors for germination of S. birrea seeds.

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Preliminary examination of factors affecting Agrobacterium tumefaciens-mediated transformation of marula, Sclerocarya birrea subsp. caffra (Anacardiacease)

Cited by 7 publications

References 34 publications

Agrobacterium-mediated transformation and shoot regeneration in elite breeding lines of western shipper cantaloupe and honeydew melons (Cucumis melo L.)

Agrobacterium-mediated transformation and shoot regeneration in elite breeding lines of western shipper cantaloupe and honeydew melons (Cucumis melo L.)

Recalcitrant effects associated with the development of basal callus-like tissue on caulogenesis and rhizogenesis in Sclerocarya birrea

After-ripening, Light Conditions, and Cold Stratification Influence Germination of Marula [Sclerocarya birrea (A. Rich.) Hochst. subsp. caffra (Sond.) Kokwaro] Seeds

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