Preliminary findings of age and male sexual characteristics andand potential effect to semen characteristics and cryopreservation of the critically endangered Bornean orangutan in Malaysia

Zainuddin, Zainal Zahari; Tarmizi, Reza; Chee, Yap Keng; Sarkawi, Nur Nabila; Ahmad, Hafandi; Salleh, Annas; Tahir, Nur Diyana Mohamad; Baiee, Falah; Che-Amat, Azlan; Fitri, Wan-Nor

doi:10.1007/s10329-022-00989-z

Cited by 2 publications

(1 citation statement)

References 30 publications

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“…We emphasize, however, that the data from the evaluation of the five animals are still highly relevant, given the scarcity of information in this species. Indeed, it is worth noting that similar sample sizes were used in previous studies to describe sperm parameters of other wild species, from critically endangered Southern muriqui (Brachyteles arachnoides) [45] and Bornean orangutans (Pongo pygmaeus) [46] to species of least concern, such as six-banded armadillos (Euphractus sexcinctus) [47] and crab-eating foxes (Cerdocyon thous) [48]. Therefore, numerous directions for future studies will come from these initial results as the description of functional aspects of the rhea sperm, such as the kinetic parameters determined by computer analysis, mitochondrial activity, and chromatin status.…”

Section: Discussionmentioning

confidence: 99%

Changes in Sperm Morphology, Morphometry, and Motility from the Epididymis to the Vas Deferens in Rheas (Rhea americana, Linnaeus, 1758)

Bezerra

Jurema

Dantas

et al. 2023

Animals

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The objective was to characterize morphological, morphometric, and ultrastructural changes in rhea spermatozoa between the epididymis and the vas deferens. Sperm samples were collected from the reproductive tracts of seven adult individuals and evaluated for sperm characteristics using brightfield microscopy as well as ultrastructural features using scanning electron microscopy (SM). Mean sperm count tended to increase in the vas deferens (378.0 ± 135.0 × 106) compared to the epididymis (201.0 ± 77.4 × 106). Percentages of motile sperm grew from 37.0 ± 4.9% in the epididymis to 58.5 ± 7.7% in the vas deferens. The proportion of normal spermatozoa was 75.6 ± 1.8% and most common defects were bent tails (9.7 ± 0.9%). However, these proportions were not different between epididymis and vas deferens. SM analysis revealed further features of rhea spermatozoa. Normal rhea spermatozoa were threadlike with an acrosome (0.95 ± 0.0 µm), head (7.53 ± 0.01 µm), midpiece (2.08 ± 0.01 µm), and tail (30.7 ± 0.06 µm). Lengths of sperm acrosome, head, midpiece, and tail were longer in the vas deferens compared to the epididymis. Our findings suggest that rhea spermatozoa undergo a maturation process during the passage from the epididymis to the vas deferens.

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Section: Discussionmentioning

confidence: 99%

Changes in Sperm Morphology, Morphometry, and Motility from the Epididymis to the Vas Deferens in Rheas (Rhea americana, Linnaeus, 1758)

Bezerra

Jurema

Dantas

et al. 2023

Animals

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Benchmarks defining high‐quality sperm in the common marmoset (Callithrix jacchus)

Sadeghi,

Mustoe,

Ross

et al. 2024

Andrology

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BackgroundCommon marmosets (Callithrix jacchus) are increasingly recognized as valuable nonhuman primates (NHPs) for biomedical research due to their small size and short reproductive cycle and lifespan relative to other NHP species. Maximizing the utility of captive research marmosets, including genetically manipulated animals, will require the use of assisted reproductive techniques (ART) including manipulation, storage, and sharing of marmoset sperm. Here, we identify characteristics of high‐quality semen samples and validate a simple method for selecting high‐quality sperm.MethodsComputer‐assisted sperm analysis (CASA) was used to evaluate sperm quality in semen samples collected from 44 marmosets and assessed the use of the swim‐up method for the selection of high‐quality sperm was also tested in half the samples as a potential means to optimize in vitro fertilization or intrauterine insemination.ResultsFor each reference parameter, samples at or below the 5th percentile were categorized as abnormal sperm, while those above the 5th percentile were considered to be normal. Among normal samples, those at or above the 50th percentile were categorized as high‐quality. High‐quality semen samples exhibited the following characteristics: semen volume ≥ 30 µL; sperm count ≥ 107/ejaculate; total motility ≥ 35%; and normal morphology ≥ 5%. Sperm isolated by swim‐up exhibited superior sperm progressive motility (19.7% ± 4.5 vs. 5.6% ± 2.1; P = 0.01) and normal morphology (13.1 ± 1.59 vs. 7.65 ± 1.1; P < 0.001) compared with unselected sperm.ConclusionThis study defines robust, statistically supported reference values for evaluating marmoset semen samples to assist with the identification of optimal sperm donors and the selection of high‐quality sperm samples for assisted reproduction. Ultimately, these reference values combined with a validated selection method will contribute to consistent standards for the international sharing of genetically diverse and/or gene‐edited marmoset sperm for research and reproduction.

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Preliminary findings of age and male sexual characteristics andand potential effect to semen characteristics and cryopreservation of the critically endangered Bornean orangutan in Malaysia

Cited by 2 publications

References 30 publications

Changes in Sperm Morphology, Morphometry, and Motility from the Epididymis to the Vas Deferens in Rheas (Rhea americana, Linnaeus, 1758)

Changes in Sperm Morphology, Morphometry, and Motility from the Epididymis to the Vas Deferens in Rheas (Rhea americana, Linnaeus, 1758)

Benchmarks defining high‐quality sperm in the common marmoset (Callithrix jacchus)

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