Preliminary findings on identification of mycorrhizal fungi from diverse orchids in the Central Highlands of Madagascar

Yokoya, K.; Zettler, Lawrence W.; Kendon, Jonathan P.; Bidartondo, Martin I.; Stice, Andrew L.; Skarha, Shannon; Corey, Laura L.; Knight, Audrey C.; Sarasan, Viswambharan

doi:10.1007/s00572-015-0635-6

Cited by 27 publications

(39 citation statements)

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“…Ultimately there is a great need to understand the importance of mycorrhizal fungi in epiphytes and, especially, lithophytes to improve reintroduction/restoration to support integrated conservation for meaningful outcomes in the longer term. At the moment very little is known about the mycorrhizae of lithophytes and epiphytes of Madagascan orchids except the preliminary studies conducted in our laboratory (Yokoya et al 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Collecting near mature and immature orchid seeds for ex situ conservation: ‘in vitro collecting’ as a case study

et al. 2017

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BackgroundLack of phenological information and efficient collecting methods are considered impediments for orchid seed collecting. This leads to opportunistic collecting as part of general seed collecting schedules that may last few weeks especially in remote areas. The study explored the feasibility of collecting near mature and immature seeds to support conservation action plans. Mature, near mature and immature seeds of orchids were collected from the wild in the Central Highlands of Madagascar (CHM). Seed capsules were collected in sterile culture medium in the wild, to prevent deterioration of seeds inside the capsule after collecting, later to be cultured under laboratory conditions.ResultsSeed capsules collected by the in vitro collecting (IVC) method were kept in very good condition for up to 4 weeks before germination under in vitro conditions. Significantly faster and higher germination rate (p < 0.001) than mature seeds was observed in a number of taxa collected during a 3 year-long study. In some taxa even immature seeds, with no apparent sign of testa covering the embryo, germinated following IVC where mature seeds failed to germinate.ConclusionsWe propose that IVC method has potential to complement conventional seed collecting by increasing the germplasm that can be used in integrated conservation action plans. Improvements can be made in developing collections for taxa from biodiversity hotspots and remote areas where collecting requires considerable resources. This method can further be used on a wider selection of plants from different geographic areas and on embryo rescue programmes for economically important plants.Electronic supplementary materialThe online version of this article (doi:10.1186/s40529-017-0187-5) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Collecting near mature and immature orchid seeds for ex situ conservation: ‘in vitro collecting’ as a case study

et al. 2017

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“…DNA was extracted from the mycelium of each cultured fungal isolate. To identify fungal isolates, the internal transcribed region of the rRNA gene (ITS) was sequenced (Yokoya et al, 2015). The DNA was amplified by PCR with primers ITS1 and ITS4 (White et al, 1990).…”

Section: Molecular Identificationmentioning

confidence: 99%

“…The current study aims to identify fungi that can be cultured and used for future seed germination, by isolating fungi from pelotons in root samples. This approach was preferred over the seed baiting methods as successful seed baiting may take years (Bidartondo & Read, 2008) while collecting root samples from plants at different maturity is faster (Yokoya et al, 2015). This may provide a list of potential symbionts that can be used for time-bound projects.…”

Section: Introductionmentioning

confidence: 99%

Diversity of root-associated culturable fungi ofCephalanthera rubra(Orchidaceae) in relation to soil characteristics

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Yokoya

Kendon

et al. 2020

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Cephalanthera rubra (L.) Rich., Red Helleborine, is a widespread orchid in Europe but known only from three very small populations in England. These populations are in decline with no natural seed setting for more than a decade. The species may become extinct in the UK soon unless viable strategies are in place for ex situ conservation, especially the use of symbiotic propagation. Because of the fragile nature of the populations in England mycorrhizal fungal diversity study is not feasible. Therefore, to understand the factors needed for healthy Red Helleborine populations, soil characteristics and diversity of culturable root-derived fungi of the populations from a small area in the Loire Valley in France were studied. The main objectives of the study were: (1) Which culturable mycorrhizal fungi associated with C. rubra roots and (2) To what extent is variation in fungal communities related to variation in soil characteristics? Here, we report a significant difference in diversity of culturable mycorrhizal and non-mycorrhizal fungi depending on soil pH and phosphorus content. Mycorrhizal associations were favoured by plants in locations with low soil nutrient availability and comparatively higher pH. Our study shows that mycorrhizal fungi, both ecto and endo, can be cultured from roots of plants at different maturity stages.

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“…Molecular identification followed the procedures outlined by Zettler et al (2013) and Yokoya et al (2015) involving ribosomal DNA internal transcribed spacer (ITS) amplification and Sanger sequencing. A 1 cm 3 Fig.…”

Section: Molecular Identificationmentioning

confidence: 99%

Further evidence of Ceratobasidium D.P. Rogers (Basidiomycota) serving as the ubiquitous fungal associate of Platanthera leucophaea (Orchidaceae) in the North American tallgrass prairie

et al. 2020

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Background: In the United States and Canada, ca. one-half of native orchid species are now threatened with extinction. A number of these species are restricted to tallgrass prairies of central North America, such as the Eastern Prairie Fringed Orchid, Platanthera leucophaea (Nutt.) Lindl.-a U.S. Federally threatened species. Results:We provide new records of fungi recovered from roots of P. leucophaea and five other orchid species inhabiting prairie sites in Illinois and neighboring states during a 10-year period (2008)(2009)(2010)(2011)(2012)(2013)(2014)(2015)(2016)(2017). A total of 39 fungal endophytes were isolated from Cypripedium candidum (1), Platanthera lacera (1), P. leucophaea (32), P. peramoena (3), Spiranthes lacera (1), and S. magnicamporum (1), 31 (79%) of which were assignable to Ceratobasidium and the remainder to Tulasnella. These fungi were acquired from 16 different sites, 13 of which are new records including two new state records (Iowa, Wisconsin). Molecular analysis revealed that some Ceratobasidium strains were virtually identical despite being geographically isolated by > 300 km. Conclusions:This study, encompassing a decade of work, confirms that Platanthera leucophaea is a mycorrhizal specialist with heavy reliance on Ceratobasidium with the tallgrass prairie ecosystem of North America. Our isolation of Ceratobasidium from P. leucophaea spanning additional sites suggests that the association is widespread. Such information should provide conservationists and land managers with more confidence in developing protocols that facilitate the long-term conservation of this prairie orchid.

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Preliminary findings on identification of mycorrhizal fungi from diverse orchids in the Central Highlands of Madagascar

Cited by 27 publications

References 35 publications

Collecting near mature and immature orchid seeds for ex situ conservation: ‘in vitro collecting’ as a case study

Collecting near mature and immature orchid seeds for ex situ conservation: ‘in vitro collecting’ as a case study

Diversity of root-associated culturable fungi ofCephalanthera rubra(Orchidaceae) in relation to soil characteristics

Further evidence of Ceratobasidium D.P. Rogers (Basidiomycota) serving as the ubiquitous fungal associate of Platanthera leucophaea (Orchidaceae) in the North American tallgrass prairie

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