Preliminary genetic analysis of sucrose accumulation in berries of table grapes

Shiraishi, Masayuki; Shinomiya, Ryo; Chijiwa, Hiroyuki

doi:10.1016/j.scienta.2012.01.024

Cited by 8 publications

(10 citation statements)

References 23 publications

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“…After determination of soluble solids content and titratable acidity, the remaining juice sample was clarified by centrifugation at 5,000 × g for 10 min for sugar composition analysis (Shiraishi et al, 2012). The resulting supernatant was diluted 10 times with deionized water and filtered through a 0.45 μm filter.…”

Section: Sugar Composition Analysismentioning

confidence: 99%

“…Biochemical and transcriptional considerations of sucrose accumulation could provide valuable information for the manipulation of sugar metabolism in grape berries for future breeding of table grapes. Shiraishi et al (2012) proposed in a preliminary genetic study a monogenic control model for the trait of sucrose accumulation in table grapes: complete dominance of hexose accumulation [A] over sucrose accumulation [a]. We also established two-schemed diploid S 1 and F 2 seedling populations from 2009 to 2011 to further test the single-gene hypothesis.…”

Section: Introductionmentioning

confidence: 99%

“…In this study, we report 1) genetic analysis on the trait of sucrose accumulation using diploid seedling populations of S 1 and F 2 , and 2) transcriptional analysis of sugar accumulation during diploid grape berry ripening in both sucrose-and hexose-accumulating grapevines, together with time-course changes in the associated berry quality traits. Table 1 shows the sugar composition of the parental cultivar/selections used in the present study, in which the type of sugar accumulation and its presumed genotype are given according to previous studies (Shiraishi et al, 2010(Shiraishi et al, , 2012. We used two-schemed diploid S 1 and F 2 seedling populations for genetic analysis of sugar accumulation (Shiraishi et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Table 1 shows the sugar composition of the parental cultivar/selections used in the present study, in which the type of sugar accumulation and its presumed genotype are given according to previous studies (Shiraishi et al, 2010(Shiraishi et al, , 2012. We used two-schemed diploid S 1 and F 2 seedling populations for genetic analysis of sugar accumulation (Shiraishi et al, 2012). One vine per seedling was grafted onto Kober 5BB rootstock and grown from 2016 to 2017 with a vine-by-row spacing of 0.8 × 2.0 m in a north-south orientation in a greenhouse at Fukuoka Agricultural and Forestry Research Center, Fukuoka, Japan (33°50' N; 130°57' E).…”

Section: Introductionmentioning

confidence: 99%

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Genetic and Gene Expression Analysis of Berry Sugar Composition in a Sucrose-accumulating Grapevine

Shiraishi

Hamada

2020

Hort. J.

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We examined hexose-and sucrose-accumulating grapevines to elucidate genetic and transcriptional control of berry sugar composition in table grapes. Sugar composition was classified into two types: a hexose accumulator that accumulates fructose, glucose, and no or trace amounts of sucrose and a sucrose accumulator that accumulates fructose, glucose, and a large amount of sucrose. Segregation ratios among diploid seedling populations of S 1 and F 2 indicate that the trait of sucrose accumulation is controlled by a single major recessive gene. Transcriptional levels of sugar accumulation-related key genes were monitored during berry ripening using two hexose-accumulating cultivar/selections and one sucrose-accumulating cultivar. Gene expression of sucrose transporter and sucrose phosphate synthase coincided with a rapid increase in sugar accumulation after the onset of berry ripening; gene expressions of those enzymes and cell wall invertase were not significantly different between sucrose-and hexose-accumulators and gene expression of vacuolar acid invertase decreased after the onset of berry ripening in both sucrose-and hexoseaccumulators. Gene expression of sucrose synthase (catalyzes the reaction of sucrose cleavage) in the hexose accumulators remained constant throughout berry development, whereas the expression in sucrose accumulators was significantly lower, with a concomitant sharp rise in sucrose accumulation. These results suggest that sucrose synthase may play a critical role in the sugar metabolism of sucrose-accumulating grape berries.

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Section: Sugar Composition Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genetic and Gene Expression Analysis of Berry Sugar Composition in a Sucrose-accumulating Grapevine

Shiraishi

Hamada

2020

Hort. J.

Self Cite

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“…Though it has the limitations of containing low levels of sugar, high malic acid concentration and significant foxy aroma content that affects negatively its preference [4]. Kyoho, the second most cultivated grape, is a tetraploid hybrid of V. labrusca and V. vinifera ('Ishihara Wase' × 'Centennial') [5][6][7] and it is famous for its large-sized berry and for its intense grape flavor [5]. Several studies have been conducted for the improvement and optimization of the wines produced from V. labrusca grapes.…”

Section: Introductionmentioning

confidence: 99%

Approaches to outline the aromatic profile of Kyoho wines from South Korea

et al. 2017

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Kyoho cultivar is a hybrid of V. vinifera and V. labrusca and is one of the major varieties cultivate in South Korea. Kyoho grapes are characterized for their intense grape flavor and sour attributes. Korean climatic conditions are different from worldwide wine producing countries. The summer season is hot and dominated by heavy rains. Wines from Kyoho grapes are distinct for their intense grape flavor and the key odors that characterize the wines are aromas of caramel and strawberry notes. The past few years efforts are put to improve the quality and to advance the potential of the domestic wines. The objective of the present study is to provide insight to the main aromatic compounds that form the aroma profile of Kyoho wines. For this aim, Gas Chromatography was coupled with Mass Spectrometry for the separation and identification of the compounds. Analysis allowed to identify the volatile compounds that form the aroma of the wine and to detect the key odors of its characteristic aroma. (2,5-dimethyl-4-hydroxy-3(2H)-furanone) Furaneol , which is associated with caramel-like flavor, was one of the compounds that were detected along with ((3R)-hydroxy-4,4-dimethyloxolan-2-one) pantolactone.

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Heterologous expression of a novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene, ZjGRP, caused salt sensitivity in Arabidopsis

et al. 2016

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A novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene was cloned in this study and its overexpression caused salt sensitivity in transgenic Arabidopsis. Glycine-rich RNA-binding proteins (GRPs) play crucial roles in diverse plant developmental processes. However, the mechanisms and functions of GRPs in salinity stress responses remain largely unknown. In this study, rapid amplification of cDNA end (RACE) PCR methods was adopted to isolate ZjGRP from Zosyia japonica, a salt-tolerant grass species. ZjGRP cDNA was 456 bp in length, corresponding to 151 amino acids. ZjGRP was localized in the nucleus and cytoplasm, and was found particularly abundantly in stomatal guard cells. Quantitative real-time PCR showed that ZjGRP was expressed in the roots, stems, and leaves of Zoysia japonica, with the greatest expression seen in the fast-growing leaves. Furthermore, expression of ZjGRP was strongly induced by treatment with NaCl, ABA, MeJA, and SA. Overexpression of ZjGRP in Arabidopsis reduced the rate of germination and retarded seedling growth. ZjGRP-overexpressing Arabidopsis thaliana exhibited weakened salinity tolerance, likely as a result of effects on ion transportation, osmosis, and antioxidation. This study indicates that ZjGRP plays an essential role in inducing salt sensitivity in transgenic plants.

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Preliminary genetic analysis of sucrose accumulation in berries of table grapes

Cited by 8 publications

References 23 publications

Genetic and Gene Expression Analysis of Berry Sugar Composition in a Sucrose-accumulating Grapevine

Genetic and Gene Expression Analysis of Berry Sugar Composition in a Sucrose-accumulating Grapevine

Approaches to outline the aromatic profile of Kyoho wines from South Korea

Heterologous expression of a novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene, ZjGRP, caused salt sensitivity in Arabidopsis

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