Preliminary<i>In Vitro</i>Evaluation of Genistein Chemopreventive Capacity as a Result of Esterification and Cyclodextrin Encapsulation

Danciu, Corina; Şoica, Codruţa; Dehelean, Cristina; Zupkó, István; Csányi, Erzsébet; Pînzaru, Iulia

doi:10.1155/2015/262930

Cited by 5 publications

(4 citation statements)

References 39 publications

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“…Melanoma cells, specifically Skmel-103, exhibited a high sensitivity to GN, as expected due to their higher metabolic activity compared to 3T3 cells, which are non-malignant. This heightened sensitivity to GN in melanoma cells has been previously reported in various studies (Danciu et al, 2015;Dobrzynska, Napierala, Florek, 2020;Ji et al, 2012;Rauth, Kichina, Green, 1997;Tyagi, Song, De, 2019;Venza et al, 2018).…”

Section: Cell Viability and Uptake Studiessupporting

confidence: 81%

“…As per Zhang et al (2015), GN typically exhibits an endothermic peak at 301.7°C, which we observed in our study at around 303.7°C. Danciu et al (2015) also reported similar findings, with GN displaying an endothermic peak at 303°C, followed by an exothermic peak at 307°C, signifying chemical decomposition. In our DSC analysis of PNTPGS-GN (Figure 2-A) and PNPol-GN (Figure 2-B) formulations, it was not detected any additional peaks, confirming that no new composites formed due to degradation, chemical reactions, or incompatibilities.…”

Section: Nanoparticle Characterizationsupporting

confidence: 62%

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Poly-ε-caprolactone based nanoparticles for delivery of genistein in melanoma treatment

Abriata,

Luiz,

Viegas

et al. 2024

Braz. J. Pharm. Sci.

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We developed poly-ε-caprolactone (PCL)-based nanoparticles containing D-α-tocopherol polyethylene glycol-1000 succinate (TPGS) or Poloxamer 407 as stabilizers to efficiently encapsulate genistein (GN). Two formulations, referred to as PNTPGS and PNPol, were prepared using nanoprecipitation. They were characterized by size and PDI distribution, zeta potential, nanoparticle tracking analysis (NTA), GN association (AE%), infrared spectroscopy (FT-IR), and differential scanning calorimetry (DSC). PNTPGS-GN exhibited a particle size of 141.2 nm, a PDI of 0.189, a zeta potential of -32.9 mV, and an AE% of 77.95%. PNPol-GN had a size of 146.3 nm, a better PDI than PNTPGS-GN (0.150), a less negative zeta potential (-21.0 mV), and an AE% of 68.73%. Thermal and spectrometric analyses indicated that no new compounds were formed, and there was no incompatibility detected in the formulations. Cellular studies revealed that Poloxamer 407 conferred less toxicity to PCL nanoparticles. However, the percentage of uptake decreased compared to the use of TPGS, which exhibited almost 80% cellular uptake. This study contributes to the investigation of stabilizers capable of conferring stability to PCL nanoparticles efficiently encapsulating GN. Thus, the PCL nanoparticle proposed here is an innovative nanomedicine for melanoma therapy and represents a strong candidate for specific pre-clinical and in vivo studies.

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Section: Cell Viability and Uptake Studiessupporting

confidence: 81%

Section: Nanoparticle Characterizationsupporting

confidence: 62%

Poly-ε-caprolactone based nanoparticles for delivery of genistein in melanoma treatment

Abriata,

Luiz,

Viegas

et al. 2024

Braz. J. Pharm. Sci.

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“…The protocol used to obtain the cyclodetrins' complex, known as the kneading procedure, was already described in our previous scientific papers [10][11][12]: the active substance and hydroxypropyl-gamma-cyclodextrin -molar ratio 1:2 -were triturated with a mortar and a pestle, followed by kneading with a 50% ethanol solution until an important part of the solvent was evaporated. In the last step, the product was dried at room temperature for 24 h and then at 105 o C in a laboratory oven for several hours, until constant weight.…”

Section: Cyclodextrin Incorporationmentioning

confidence: 99%

Toxicological Evaluation of Some Essential Oils Obtained from Selected Romania Lamiaceae Species in Complex with Hydroxypropyl - gamma-cyclodextrin

2019

Rev.Chim.

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Medicinal plants are highly investigated due to their multiple effects in human health. In the last two decades, plants belonging to Lamiaceae family have emerged as an important source of compounds with beneficial effects in various pathologies. Many species of the Lamiaceae family have a high volatile oil content which can be found in different parts of the plant. Some representative examples include Lavandula spp. Salvia spp. and Mentha spp. which are species with essential oils studied for antioxidant, antimicrobial and antitumor effects. The aim of this study was to evaluate the effect of six essential oils at skin level before and after encapsulation in hydroxypropyl-gamma-cyclodextrins. Results indicated that regardless of the tested form, the samples did not display an irritating or toxic effect at skin level, suggesting that the essential oils can be used in different formulations for topical application. Keywords: essential oils, Lamiaceae, hydroxypropyl-gamma-cyclodextrins, mexametry, corneometry, tewametry

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“…The new formulations did not decrease the viability of the cancerous cells. This behavior may be explained by the increased stability of the complex within the in vitro environment [138].…”

Section: Anti-inflammatory and Chemopreventive Activity Of Isoflavonomentioning

confidence: 99%

New Insights Regarding the Potential Health Benefits of Isoflavones

Danciu¹,

Suciu²,

Antal³

et al. 2017

Flavonoids - From Biosynthesis to Human Health

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Isoflavones are a class of plant secondary metabolites, with an estrogen-like structure presenting a plethora of biological activities. The chapter discusses important facts about this class of phytoestrogens, from biosynthesis to the latest research about their health benefits. The following major points discussed are: biosynthesis, regulation, isolation, metabolism and bioavailability, isoflavones in diet and intake, and new insights regarding the therapeutic effect including cancer chemoprevention. The chapter ends with a mini review of own research of the anti-inflammatory and chemopreventive activity of isoflavonoid genistein alone and incorporated in modern pharmaceutical formulations. The chapter updates the interested researchers in the field with the latest progress regarding potential health benefits of isoflavones.

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PreliminaryIn VitroEvaluation of Genistein Chemopreventive Capacity as a Result of Esterification and Cyclodextrin Encapsulation

Cited by 5 publications

References 39 publications

Poly-ε-caprolactone based nanoparticles for delivery of genistein in melanoma treatment

Poly-ε-caprolactone based nanoparticles for delivery of genistein in melanoma treatment

Toxicological Evaluation of Some Essential Oils Obtained from Selected Romania Lamiaceae Species in Complex with Hydroxypropyl - gamma-cyclodextrin

New Insights Regarding the Potential Health Benefits of Isoflavones

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