Preliminary in vitro study of anti-oxidant activity and anti-diabetic potential of plant extracts from 4 herbal substances not traditionally used for treatment of diabetes mellitus

Trifonova, Dora; Gavrilova, Anna; Dyakova, Galina; Genadi, Gavrilov,; Yotova, Maya; Nikolov, S

doi:10.3897/pharmacia.68.e72769

Cited by 5 publications

(7 citation statements)

References 51 publications

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“…Our measured TPC values (from both aqueous and ethyl acetate extracts) were comparable with some previously obtained 14,15,45 (Table 2), but higher than the values for EtOAc and n-ButOH extracts of aerial parts in 13 .…”

Section: Discussionsupporting

confidence: 89%

“…The scavenging effect of both aqueous extracts of S. kitaibelii on the ABTS was also more pronounced comparing to the ethyl acetate. Aqueous extracts from leaves and owers showed the strongest free ABTS radicals scavenging ability (2.834 mg AA/g), these values are comparable to those obtained by 45 . According to our knowledge, there is no comparable data on this activity of S. kitaibelii extracts.…”

Section: Discussionsupporting

confidence: 77%

“…Ethanol extracts of S. kitaibelii aerial parts showed higher SC 50 values -102.24 and 106.94 µg/mL 15 . Aqueous extracts obtained with ultrasonic extraction with gradual increase in temperature show IC 50 value 0.087 mg/mL 45 . The scavenging effect of both aqueous extracts of S. kitaibelii on the ABTS was also more pronounced comparing to the ethyl acetate.…”

Section: Discussionmentioning

confidence: 99%

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Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff

Gopčević

Grujić

Arsenijević

et al. 2022

Preprint

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This study aimed at investigating biological activity and chemical composition of Satureja kitaibelii Wierzb. ex Heuff. LC-PDA/MS analyses for the aqueous (A1-ste, leaves and flowers, A2-leves and flowers) and ethyl-acetate (E1-stem, leaves and flowers, E2-leaves and flowers) extracts obrtained by ultrasound-assisted extraction allowed the identification of thirty four compounds. Quantitative analysis revealed that the aqueous extract obtained from leaves and flowers was the richest in total phenolic acids (65.36 mg/g) and flavonoids (21.17 mg/g). Total polyphenol content was the highest in the aqueous extract obtained from leaves and flowers (27±2.4 mg Gallic Acid equivalents /g). The best antioxidant activity was observed for the same extract using the DPPH (SC50 20±10 µg/mL), ABTS (2.834±0.02 mg Ascorbic Acid/g), FRAP (1.922±0.03 mmol Fe2+/mg), and total reducing power tests (16.4±1.0 mg Ascorbic Acid/g). Both ethyl acetate extracts were the most active against strains of Bacillus cereus and Micrococcus flavus (MIC 1.70-1.99 mg/mL and 1.99-3.41 mg/mL, respectively). They were more efficient against Aspergillus ochraceus (MFC 0.86 mg/mL) and towards HeLa cell lines. All obtained results implied the good potential of investigated extracts to be used as an effective preservatives and functional ingredients in food products and dietary supplements.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff

Gopčević

Grujić

Arsenijević

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Ethanol extracts of S. kitaibelii aerial parts showed higher SC 50 values-102.24 and 106.94 µg/mL 15 . Aqueous extracts obtained with ultrasonic extraction with a gradual increase in temperature show IC 50 value of 0.087 mg/mL 51 . The scavenging effect of both aqueous extracts of S. kitaibelii on the ABTS was also more pronounced compared 51 .…”

Section: Discussionmentioning

confidence: 99%

“…Aqueous extracts obtained with ultrasonic extraction with a gradual increase in temperature show IC 50 value of 0.087 mg/mL 51 . The scavenging effect of both aqueous extracts of S. kitaibelii on the ABTS was also more pronounced compared 51 . To our knowledge, there is no comparable data on this activity of S. kitaibelii extracts.…”

Section: Discussionmentioning

confidence: 99%

Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff. obtained by ultrasound-assisted extraction

Gopčević

Grujić

Arsenijević

et al. 2022

Sci Rep

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The aim of the study was to investigate the biological activity and chemical composition of Satureja kitaibelii Wierzb. ex Heuff. LC–PDA/MS analyses for the aqueous extracts (A1-stem, leaves and flowers, A2-leaves and flowers) and ethyl-acetate extracts (E1-stem, leaves and flowers, E2-leaves and flowers) obtained by ultrasound-assisted extraction enabled the identification of thirty-four compounds. Quantitative analysis revealed that the aqueous extract obtained from leaves and flowers was the richest in total phenolic acids (65.36 mg/g) and flavonoids (21.17 mg/g). The total polyphenol content was the highest in the aqueous extract obtained from leaves and flowers (274 ± 2.4 mg Gallic Acid equivalents/g). The best antioxidant activity was observed for the same extract using the DPPH (SC50 20 ± 10 µg/mL), ABTS (2.834 ± 0.02 mg Ascorbic Acid/g), FRAP (1.922 ± 0.03 mmol Fe2+/mg), and total reducing power tests (16.4 ± 1.0 mg Ascorbic Acid/g). Both ethyl acetate extracts were the most active against strains of Bacillus cereus and Micrococcus flavus (MIC 1.70–1.99 mg/mL and 1.99–3.41 mg/mL, respectively). They were more efficient against Aspergillus ochraceus (MFC 0.86 mg/mL) and towards HeLa cell lines. All the obtained results implied the good potential of the investigated extracts to be used as effective preservatives and functional ingredients in food products and dietary supplements.

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Phytochemical Screening of Satureja Kitaibelii Wierzb. Ex Heuff. Extracts by Gc/MS and TLC

GAVRILOVA¹

2023

FARMACIA

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The Balkan endemic species Satureja kitaibelii Wierzb. ex Heuff. is known in the folk medicine as herbal remedy, spice and natural food preservative. It has many biological properties such as antimicrobial, antioxidant, anti-inflammatory, etc. The purpose of this study was the GC/MS and TLC analyses of Satureja kitaibelii extracts of Bulgarian origin. Th plant surface exudate, the methanolic extract and a fraction prepared by alkaline hydrolysis were obtained the aerial parts. Phenolic acids, flavonoid aglycones (methyl derivatives of flavones), triterpene acids, terpenoids and fatty acids were identified in the plant surface exudate. Carvacrol, oleanolic acid, ursolic acid and 4-hydroxybenzoic acid were determined as main compounds in the plant surface exudate. Phenolic acids as quinic and rosmarinic acids, and carvacrol and palmitic acid prevailed in the methanolic extract. The most prevalent methanol insoluble phenolic acids further extracted with ethyl acetate after alkaline hydrolysis were caffeic and 4-hydroxycinnamic acids, followed by ferulic, syringic, 4-hydroxybenzoic and 3,5-dihydroxybenzoic acids. The investigated S. kitaibelii extracts showed a high content of important biologically active substances. RezumatSpecia endemică balcanică Satureja kitaibelii Wierzb. ex Heuff. este cunoscută în medicina populară ca remediu empiric, ca și condiment sau conservant alimentar natural. Are multe proprietăți biologice, cum ar fi efectul antimicrobian, antioxidant, antiinflamator, etc. Scopul acestui studiu a fost analiza GC/MS și TLC a extractelor de Satureja kitaibelii din Bulgaria. S-au obținut exudatul de suprafață al plantei, extractul metanolic și o fracțiune preparată prin hidroliză alcalină din părțile aeriene. În exudatul de suprafață au fost identificați acizi fenolici, agliconi flavonoizi (derivați metilici ai flavonelor), acizi triterpenici, terpenoizi și acizi grași. Carvacrolul, acidul oleanolic, acidul ursolic și acidul 4-hidroxibenzoic au fost determinați ca și compuși principali în exudatul de suprafață al plantei. Acizii fenolici, precum acizii chinic și rosmarinic, carvacrolul și acidul palmitic au predominat în extractul metanolic. Cei mai răspândiți acizi fenolici insolubili în metanol extrași cu acetat de etil după hidroliză alcalină au fost acizii cafeic și 4-hidroxicinamic, urmați de acizii ferulic, siringic, 4-hidroxibenzoic și 3,5dihidroxibenzoic. În concluzie, extractele de S. kitaibelii investigate au arătat un conținut ridicat în substanțe biologic active.

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Preliminary in vitro study of anti-oxidant activity and anti-diabetic potential of plant extracts from 4 herbal substances not traditionally used for treatment of diabetes mellitus

Cited by 5 publications

References 51 publications

Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff

Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff

Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff. obtained by ultrasound-assisted extraction

Phytochemical Screening of Satureja Kitaibelii Wierzb. Ex Heuff. Extracts by Gc/MS and TLC

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