Preliminary Investigation of Essentially Derived Variety of Tea Tree and Development of SNP Markers

li, LI; Li, Xiangru; Liu, Fei; Zhao, Jialin; Zhang, Yan; Zheng, Wenjie; Fan, Li

doi:10.3390/plants12081643

Cited by 3 publications

(1 citation statement)

References 41 publications

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“…For example, fertilization, irrigation, pest control, and pruning, while effective in improving tea yield and quality, still fail to meet market demand [ 6 , 7 , 8 , 9 ]. Therefore, some scholars believe that the key to maintaining the sustainable development of the Wuyi Rock tea industry is to optimize the selection of high-yield and high-quality tea germplasm resources [ 10 , 11 ]. However, under natural conditions, artificial cultivation of high-quality tea germplasm resources often requires long cycles, which is still a drawback to the rapid promotion of the Wuyi Rock tea industry [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Aviation Mutagenesis Alters the Content of Volatile Compounds in Dahongpao (Camellia sinensis) Leaves and Improves Tea Quality

Ye,

Zhang,

Cheng

et al. 2024

Foods

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Aviation mutagenesis is a fast and efficient breeding method. In this study, we analyzed the effect of aviation mutagenesis on volatile compounds and odor characteristics in Dahongpao fresh leaves and gross tea for the first time. The results showed that aviation mutagenesis significantly increased the total volatile compounds of Dahongpao fresh leaves and gross tea. Aviation mutagenesis most critically significantly increased the content of beta-myrcene in Dahongpao fresh leaves, prompting its conversion to beta-pinene, cubebol, beta-phellandrene, zingiberene, (Z,Z)-3,6-nonadienal, and 6-pentyloxan-2-one after processing, which in turn enhanced the fruity, green, spicy, and woody odor characteristics of the gross tea. This study provided a reference for further exploration of aviation mutagenic breeding of Camellia sinensis.

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Section: Introductionmentioning

confidence: 99%

Aviation Mutagenesis Alters the Content of Volatile Compounds in Dahongpao (Camellia sinensis) Leaves and Improves Tea Quality

Ye,

Zhang,

Cheng

et al. 2024

Foods

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Methodological approaches to gene identification of tea raw materials and raw material composition of tea-based soft drinks

Vafin,

Mikhailova,

Ageykina

2024

Food systems

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Tea or tea shrub is a plant of the Camellia sinensis species, the leaves of which, previously prepared in a special way, are the traditional raw material for the production of tea products. Varietal gene identification of tea allows us to increase the level of assessment of the authenticity of tea raw materials and tea products. It is predominantly based on DNA technologies for the detection and interpretation of SNP markers (Single Nucleotide Polymorphism), represented by a wide arsenal of both expensive high-tech methods and publicly available laboratory approaches. Species gene identification of the raw material composition of tea-based soft drinks is an equally important area of research due to the risk of falsification of this type of product. The purpose of this study was to find methodological approaches to the varietal gene identification of tea raw materials and to the species gene identification of the raw material composition of tea-based soft drinks. As a result of a bioinformatics study to identify polymorphic restriction sites in the nucleotide sequences of Camellia sinensis genome loci, diagnostically significant restriction enzymes were selected that were capable of detecting SNPs and identifying tea genotypes using the analyzed markers. At the same time, 16 loci had potential for practical application, of which 11 belonged to the group of the most informative SNP markers. A post-analytical assessment of tea varieties was carried out with them regarding their genotypic affiliation and identifiability as part of solving the first task of the study. To achieve the second task, a molecular genetic approach to the species identification of the raw composition of soft drinks based on green tea was tested. The study included the analysis of experimental drinks (with natural flavoring “Lemon” and synthetic flavoring “Peach 716”), as well as commercial concentrates “TIAKVA” (based on extracts from the coarse stems of green or black tea). The methods used in the work were PCR (Polymerase Chain Reaction), RFLP (Restriction Fragment Length Polymorphism) and direct sequencing of the amplified chloroplast DNA locus. The combination of two methods (PCR and sequencing) showed its effectiveness in establishing the belonging of the analyzed nucleic acid samples to the Camellia sinensis species, the raw material base of the studied drinks and concentrates. However, to unlock the authentication potential of PCR with primers #1 and #2 combined with RFLP analysis, it will be necessary to select diagnostically significant restriction enzymes suitable for generating species-specific combinations of PCR-RFLP profiles of marker sequence.

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Availability Evaluation and Application of MNP (Multiple Nucleotide Polymorphism) Markers in Variety Identification of Chrysanthemum

Liu,

Zhao,

et al. 2024

Horticulturae

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In China, PBR (Plant Breeder’s Right) applications of chrysanthemum rank first among all of the applications of ornamental crops in China due to the plant’s significant ornamental, edible, and medicinal values. However, issues of variety infringement and disputes have become increasingly prominent, and traditional molecular markers are difficult to use due to the high heterozygosity and complex ploidy of chrysanthemum. Our study explored the availability of MNP (Multiple Nucleotide Polymorphism) markers in this regard. In total, 30 representative varieties of five types were selected for the screening of MNPs, and another 136 varieties were selected for validation of the screened MNPs. Based on ddRAD-seq (Double Digest Restriction site-associated DNA sequencing) of the 30 varieties, 487 MNPs were screened from the 26,147 SNPs that qualified, with a length from 139 to 274 bp, an average of 6.6 SNPs individually, and a repeatability rate of 99.73%. Among the 487 MNPs, 473 MNP markers were found to cover all 27 chromosomes of chrysanthemum. Performance of our MNPs in the 136 varieties was similar to those in the 30 varieties, where the average Ho (observed heterozygosity) was 71.48%, and the average DP (discriminative power) was 82.77%, preliminarily indicating the stability of the 487 MNPs. On the other hand, clustering results based on the 487 MNPs were also generally consistent with those based on the 26,147 SNPs, as well as those based on phenotypic traits, and initial grouping, likewise, further indicating the robust capability of our MNPs in variety discrimination, which is similar to their correspondence with numerous SNPs. Therefore, our MNP markers have great potential in the accurate and rapid identification of chrysanthemum varieties, and, accordingly, in fostering breeding innovation and promoting chrysanthemum marketing.

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Preliminary Investigation of Essentially Derived Variety of Tea Tree and Development of SNP Markers

Cited by 3 publications

References 41 publications

Aviation Mutagenesis Alters the Content of Volatile Compounds in Dahongpao (Camellia sinensis) Leaves and Improves Tea Quality

Aviation Mutagenesis Alters the Content of Volatile Compounds in Dahongpao (Camellia sinensis) Leaves and Improves Tea Quality

Methodological approaches to gene identification of tea raw materials and raw material composition of tea-based soft drinks

Availability Evaluation and Application of MNP (Multiple Nucleotide Polymorphism) Markers in Variety Identification of Chrysanthemum

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