Preliminary investigation of ixodid ticks in Jiangxi Province of Eastern China

Zheng, Weiqing; Xuan, Xuenan; Fu, Renlong; Tao, Huiying; Xu, Run; Liu, Yangqing; Liu, Xiaoqing; Jiang, Jia-Fu; Wu, Haixia; Ma, Hongmei; Sun, Yi; Chen, Haiying

doi:10.1007/s10493-018-0324-1

Cited by 10 publications

(6 citation statements)

References 18 publications

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“…Seven specimens of A. testudinarium were collected from seven subdistricts in Pema Gatshel, and one unidentified specimen of Ixodes was collected from a subdistrict in Trashigang. Amblyomma testudinarium is considered to be a rare tick species reported only from Asian countries like Malaysia, India, Japan, Korea, and China [47,77,78]. In India, it has been collected from cattle, mithuns, yaks, and wild animals such as the tiger, wild boar, barking deer, and elephant in Assam, Arunachal Pradesh, Mizoram, and West Bengal [47,79].…”

Section: Discussionmentioning

confidence: 99%

Identification, Distribution, and Habitat Suitability Models of Ixodid Tick Species in Cattle in Eastern Bhutan

et al. 2021

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Tick infestation is the most reported parasitological problem in cattle in Bhutan. In May and June 2019, we collected ticks from 240 cattle in two districts of Eastern Bhutan. Tick presence, diversity, and infestation prevalence were examined by morphological identification of 3600 live adult ticks. The relationships between cattle, geographic factors, and infestation prevalence were assessed using logistic regression analyses. Habitat suitability for the tick species identified was determined using MaxEnt. Four genera and six species of ticks were found. These were Rhipicephalus microplus (Canestrini) (70.2% (95% confidence interval (CI): 68.7–71.7)), Rhipicephalus haemaphysaloides Supino (18.8% (95% CI: 17.5–20.1)), Haemaphysalis bispinosa Neumann (8.2% (95% CI: 7.3–9.1)), Haemaphysalis spinigera Neumann (2.5% (95% CI: 2–3)), Amblyomma testudinarium Koch (0.19% (95% CI: 0.07–0.4)), and a single unidentified Ixodes sp. Logistic regression indicated that the variables associated with infestation were: longitude and cattle age for R. microplus; latitude for R. haemaphysaloides; and altitude and cattle breed for H. bispinosa and H. spinigera. MaxEnt models showed land cover to be an important predictor for the occurrence of all tick species examined. These findings provide information that can be used to initiate and plan enhanced tick surveillance and subsequent prevention and control programs for ticks and tick-borne diseases in cattle in Bhutan.

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Section: Discussionmentioning

confidence: 99%

Identification, Distribution, and Habitat Suitability Models of Ixodid Tick Species in Cattle in Eastern Bhutan

et al. 2021

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“…The Asian longhorned tick, Haemaphysalis longicornis, is widely distributed in eastern Asia, Australia, and New Zealand and was recently found in the US (Heath, 2016;Rainey et al, 2018;Raghavan et al, 2019;Wormser et al, 2019;Zheng et al, 2019). H. longicornis, known as a harmful ectoparasite for domestic animals, spreads diseases including babesioses to livestock (McFadden et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Porin Expression Profiles in Haemaphysalis longicornis Infected With Babesia microti

et al. 2020

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The porin gene is widely disseminated in various organisms and has a pivotal role in the regulation of pathogen infection in blood-sucking arthropods. However, to date, information on the porin gene from the Haemaphysalis longicornis tick, an important vector of human and animal diseases, remains unknown. In this study, we identified the porin gene from H. longicornis and evaluated its expression levels in Babesia microtiinfected and-uninfected H. longicornis ticks at developmental stages. We also analyzed porin functions in relation to both tick blood feeding and Babesia infection and the relationship between porin and porin-related apoptosis genes such as B-cell lymphoma (Bcl), cytochrome complex (Cytc), caspase 2 (Cas2), and caspase 8 (Cas8). The coding nucleotide sequence of H. longicornis porin cDNA was found to be 849 bp in length and encoded 282 amino acids. Domain analysis showed the protein to contain six determinants of voltage gating and two polypeptide binding sites. Porin mRNA levels were not significantly different between 1-day-laid and 7-day-laid eggs. In the nymphal stage, higher porin expression levels were found in unfed, 12-h-partially-fed (12 hPF), 1-day-partially-fed (1 dPF), 2 dPF nymphs and nymphs at 0 day post-engorgement (0 dAE) vs. nymphs at 2 dAE. Cytc and Cas2 mRNA levels were higher in 2 dPF nymphs in contrast to nymphs at 2 dAE. Porin expression levels appeared to be higher in the infected vs. uninfected nymphs during blood feeding except at 1 dPF and 0-1 dAE. Especially, the highest B. microti burden negatively affected porin mRNA levels in both nymphs and female adults. Porin knockdown affected body weight and Babesia infection levels and significantly downregulated the expression levels of Cytc and Bcl in H. longicornis female ticks. In addition, this study showed that infection levels of the B. microti Gray strain in nymphal and female H. longicornis peaked at or around engorgement from blood feeding to post engorgement. Taken together, the research conducted in this study suggests that H. longicornis porin might interfere with blood feeding and B. microti infection.

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“…Haemaphysalis longicornis is the most frequently collected species in the grassland from Jiangxi, China (Zheng et al 2019) and has become invasive in multiple regions of the world, mainly because of its parthenogenetic reproduction, broad habitat use and high diversity of avian and mammalian hosts (Heath 2016). Haemaphysalis flava was the most collected species in the grasses; it is a vector of various infectious diseases in China (Li et al 2017).…”

Section: Discussionmentioning

confidence: 99%

“…The tick Haemaphysalis flava Neumann is an important ectoparasite, which not only damages its hosts directly but also serves as a vector for various infectious pathogens in China (Cheng et al 2013, Lu et al 2013, Li et al 2017. Recent studies have also reported Haemaphysalis longicornis Neumann and Haemaphysalis hystricis Supino in China (Lu et al 2017, Wang et al 2018, Zhang et al 2018, Li et al 2019b, Yang et al 2019, Zheng et al 2019. Haemaphysalis bispinosa Neumann has been believed to occur in China, especially in the southern part.…”

mentioning

confidence: 99%

Molecular identification of Colpodella sp. of South China tiger Panthera tigris amoyensis (Hilzheimer) in the Meihua Mountains, Fujian, China

Chiu¹,

Sun²,

Bao³

et al. 2022

FOLIA PARASIT

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A three-year-old male South China tiger died in the tiger enclosure of the China Tiger Park in the Meihua Mountains on December 2018 after being bitten by a tick. This tiger presented clinical symptoms like whole-body severe jaundice, hepatosplenomegaly, kidney, and lymph node hemorrhages. The Colpodella sp.-specific 18S rRNA gene was detected using nested PCR. Interestingly, the DNA isolated from the blood of the tiger was found to be 100% similar to that of the tick by NCBI BLAST analysis. However, the DNA fragments isolated from the tiger's blood were 90.1% similar to the Colpodella sp. strain human erythrocyte parasite (HEP, MH208621) and 90.4% similar to the Colpodella sp. strain Heilongjiang (HLJ, KT364261). To investigate the species of ticks and ticks-carried Colpodella parasites in this region, the species of ticks obtained from the grasses outside the tiger enclosure and the species of Colpodella carried by ticks were identified. The DNA from ticks as well as that from the tick-borne Colpodella sp. were amplified from each tick using PCR followed by amplicon sequencing. In total 402 adult ticks samples were collected, among which 22 were positive for Colpodella sp. (5.5%), and the species were further determined by morphology, DNA sequencing and phylogenetic analyses. Interestingly, one Colpodella sp. was found to have 94.2% sequence similarities to the Colpodella sp. strain HEP (MH208621). This strain was previously reported to infect a woman in Yunnan, China. In addition, three Colpodella sp. showed 87-91% sequence similarities to the Colpodella sp. strain HLJ (KT364261), which was previously reported to infect human in Heilongjiang, China. This study disclosed the possibility of zoonotic transmission of Colpodella sp. by ticks in China. Finally, it provides a basis for urgently determining and monitoring the repertoire of ticks-borne piroplasmid pathogens, with the ultimate aim of strategic control.

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Preliminary investigation of ixodid ticks in Jiangxi Province of Eastern China

Cited by 10 publications

References 18 publications

Identification, Distribution, and Habitat Suitability Models of Ixodid Tick Species in Cattle in Eastern Bhutan

Identification, Distribution, and Habitat Suitability Models of Ixodid Tick Species in Cattle in Eastern Bhutan

Porin Expression Profiles in Haemaphysalis longicornis Infected With Babesia microti

Molecular identification of Colpodella sp. of South China tiger Panthera tigris amoyensis (Hilzheimer) in the Meihua Mountains, Fujian, China

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