Preliminary Investigations into the use of a Human Bronchial Cell Line (16HBE14o-) to Screen for Respiratory Toxins In Vitro

Westmoreland, Carl; Walker, Tracy M.; Matthews, J. C.; Murdock, Janice

doi:10.1016/s0887-2333(99)00064-8

Cited by 14 publications

(5 citation statements)

References 5 publications

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“…Limitations of this model include lack of pseudo‐stratification, deficiencies in some biotransforming enzymes, no cilia as well as, some uncharacteristic phenotypes due to carcinogenic origin of cell type 22, 26 Virus‐transformed cell lines, such as the 16HBE14o‐cells, which are human bronchial epithelia cells transformed by SV40,27 originally developed to study the cystic fibrosis conductance regulator (CFTR) 28. They form polarised, multilayered cultures, containing tight junctions and express many drug‐transporters and related proteins 22, 29, 30.…”

Section: Introductionmentioning

confidence: 99%

“…Virus‐transformed cell lines, such as the 16HBE14o‐cells, which are human bronchial epithelia cells transformed by SV40,27 originally developed to study the cystic fibrosis conductance regulator (CFTR) 28. They form polarised, multilayered cultures, containing tight junctions and express many drug‐transporters and related proteins 22, 29, 30.…”

Section: Introductionmentioning

confidence: 99%

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Tissue‐Specific Stem Cell Differentiation in an in vitro Airway Model

Prytherch

Job

Marshall

et al. 2011

Macromolecular Bioscience

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The respiratory tract is the primary site of exposure to airborne compounds, with the bronchial epithelium providing one of the first lines of defence. A growing need exists for an accurate in vitro model of the bronchial epithelium. Here, normal human bronchial epithelial (NHBE) cells cultured at an air/liquid interface create a fully differentiated, in-vivo-like model of the human bronchial epithelium. Developmental characterisation includes (i) trans-epithelial electrical resistance, (ii) morphology and (iii) bronchial cell specific stains/markers. It is concluded that the basal/progenitor cells create a pseudo-stratified, mucociliary NHBE model containing basal, serous, Clara, goblet and ciliated cells, reflective of the normal human bronchial epithelium (days 24-33 ALI culture).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tissue‐Specific Stem Cell Differentiation in an in vitro Airway Model

Prytherch

Job

Marshall

et al. 2011

Macromolecular Bioscience

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“…The dissolved MTT is converted into an insoluble purple formazan by cleavage of the tetrazolium ring by dehydrogenase enzymes [18]. Toxicity measurements on 16HBE14ocells have been described previously [ 19]. In the current study there appears to be no cellular toxic effects to the cell layers as assessed by carrying out the deposition procedure using the ATI device in the absence of microparticulates or indeed directly after application of any of the formulations to the cell layers as assessed by MTT reduction.…”

Section: Morimoto Et Almentioning

confidence: 64%

In vivo and in vitro characterization of novel microparticulates based on hyaluronan and chitosan hydroglutamate

et al. 2001

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This study examined the application of previously characterized microparticles composed of hyaluronan (HA) and chitosan hydroglutamate (CH) as well as novel microparticles consisting of both polymers (HA/CH) to improve the nasal delivery of a model drug. The rabbit bioavailabilities of gentamicin incorporated in HA, CH, and HA/CH microparticles were increased 23-, 31-, and 42-fold, respectively, compared with the control intranasal solution of gentamicin, indicating that all test microparticles were retained for longer periods on the nasal mucosa of the rabbits as supported by previous in vitro dissolution as well as frog palate mucoadhesion studies, thereby improving drug absorption. The higher bioavailabilities of CH-based formulations (CH and HA/CH) suggest the penetration-enhancing effects of CH may also be partially r esponsible for the improvement. A model was developed, based on a glass impinger device, to deliver dry powder formulations reproducibly onto the surface of cultured cell monolayers. In vitro permeability and fluorescence microscopy studies on the tight junctions of the 16HBE14o-cell lines further confirmed the ability of CH-based formulations to enhance penetration. Furthermore, the in vitro absorption profile from cell culture studies was consistent with those determined from in vivo studies. The complementary effect from the mucoadhesive nature of HA coupled with the penetration-enhancing effects of CH makes the novel HA/CH formulation a promising nasal delivery system.

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“…There are a variety of transformed, cancerous, and primary bronchial epithelial cells that have been used to investigate drug and particle diffusion. A bronchial epithelial cell line, 16HBE14o-, was immortalized with Simian Vacuolating Virus (SV40) large T-antigen to obtain cells that proliferate indefinitely[91]. The 16HBE14o- cells have differentiated epithelial morphology and functional tight junctions, but do not secrete airway mucins MUC5AC or MUC2 [92].…”

Section: Incorporation Of Mucus or Mucus-producing Cells In In Vitro mentioning

confidence: 99%

Mucus models to evaluate the diffusion of drugs and particles

Lock

Carlson

2018

Advanced Drug Delivery Reviews

168

119

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Mucus is a complex hydrogel that acts as a natural barrier to drug delivery at different mucosal surfaces including the respiratory, gastrointestinal, and vaginal tracts. To elucidate the role mucus plays in drug delivery, different in vitro, in vivo, and ex vivo mucus models and techniques have been utilized. Drug and drug carrier diffusion can be studied using various techniques in either isolated mucus gels or mucus present on cell cultures and tissues. The species, age, and potential disease state of the animal from which mucus is derived can all impact mucus composition and structure, and therefore impact drug and drug carrier diffusion. This review provides an overview of the techniques used to characterize drug and drug carrier diffusion, and discusses the advantages and disadvantages of the different models available to highlight the information they can afford.

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Preliminary Investigations into the use of a Human Bronchial Cell Line (16HBE14o-) to Screen for Respiratory Toxins In Vitro

Cited by 14 publications

References 5 publications

Tissue‐Specific Stem Cell Differentiation in an in vitro Airway Model

Tissue‐Specific Stem Cell Differentiation in an in vitro Airway Model

In vivo and in vitro characterization of novel microparticulates based on hyaluronan and chitosan hydroglutamate

Mucus models to evaluate the diffusion of drugs and particles

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