Preliminary Molecular Studies of the First Report of Burkholderia pseudomallei Isolation from Soil Collected in the Amapá State, in Northern Brazil

Sousa, V Y K; Segovia, J. F. O.; Martins, Patricia; Bezerra, Roberto Messias; Gonçalves, Magda Ca; Pereira, S W M; Vallim, Deyse Christina; Carvalho-Assef, Ana Paula D’Alincourt; Hofer, Ernesto; Kanzaki, Luis Isamu Barros

doi:10.5539/ijb.v8n1p9

Cited by 4 publications

(1 citation statement)

References 17 publications

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“…Besides the environmental isolation of B. pseudomallei reported in Peru and Brazil in 1977 [ 25 ], it has also been isolated in the Brazilian states of Ceará [ 31 ] and Amapá [ 32 ], located in the Northern region. The first attempts at environmental isolation in Brazil were made in 1973 in rice plantations in rural São Paulo, but these were unsuccessful [ 33 ].…”

Section: Review Of Published Melioidosis Cases and The Presence Ofmentioning

confidence: 99%

Melioidosis in South America

et al. 2018

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Melioidosis is an emerging disease in the Americas. This paper reviews confirmed cases, the presence of Burkholderia pseudomallei and the organization of national surveillance policies for melioidosis in South America. Confirmed cases in humans have been reported from Ecuador, Venezuela, Colombia, Brazil, and Peru. The bacterium has been isolated from the environment in Brazil and Peru. The state of Ceará, northeastern region of Brazil, is the only place where specific public strategies and policies for melioidosis have been developed. We also discuss the urgent need for health authorities in South America to pay greater attention to this disease, which has the potential to have a high impact on public health, and the importance of developing coordinated strategies amongst countries in this region.

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Section: Review Of Published Melioidosis Cases and The Presence Ofmentioning

confidence: 99%

Melioidosis in South America

et al. 2018

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Biology of the non-parasitic phase of the cattle tick Rhipicephalus (Boophilus) microplus in an area of Amazon influence

Sales,

Silva-Junior,

Tavares

et al. 2024

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Background Rhipicephalus (Boophilus) microplus is the most important tick species affecting cattle in the world. Under field conditions, the non-parasitic phase of R. (B.) microplus is unknown in the Amazon biome, including Brazil. The present study aimed to evaluate the non-parasitic phase of R. (B.) microplus in field (grass plots) and laboratory conditions. Methods The study was conducted from September 2020 to April 2022 in an Amazonian region (Maranhão State, Brazil). We evaluated the biological parameters of R. (B.) microplus under laboratory and field conditions. Engorged females were exposed to experimental conditions every 14 days, totaling 20 months of study. The following biological parameters were observed: pre-oviposition period, egg mass incubation period, and maximum larval survival period. Results Abiotic data (e.g., temperature and humidity) varied little throughout the year. Precipitation was the factor that varied the most throughout the year (dry ~ 30 mm3 and rain 400 mm3), and the parameters of pre-oviposition and pre-hatching are longer during the rainy season. A possible negative effect of the dry season on the percentage of hatched eggs was observed. Larval longevity in the plots of both control and free females was short (mean ~ 50–60 days), below that recorded for larvae under controlled conditions (mean ~ 95 days). Conclusions Rhipicephalus (Boophilus) microplus was able to complete its non-parasitic phase by producing host-seeking larvae in the pasture during all months of the study. The results indicate that R. (B.) microplus can complete up to six generations per year in biome Amazon. To our knowledge, this is the highest number of annual generations for R. (B.) microplus in Latin America. Graphical Abstract

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Evaluation of consensus method for the culture of Burkholderia pseudomallei in soil samples from Laos

et al. 2018

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Background: We have previously shown that PCR following enrichment culture is the most sensitive method to detect Burkholderia pseudomallei in environmental samples. Here we report an evaluation of the published consensus method for the culture of B. pseudomallei from Lao soil in comparison with our conventional culture method and with PCR with or without prior broth enrichment. Methods: One hundred soil samples were collected from a field known to contain B. pseudomallei and processed by: (i) the conventional method, (ii-iii) the consensus method using media prepared in either Laos or Thailand, and (iv) the consensus method performed in Thailand, as well as by (v) PCR following direct extraction of DNA from soil and (vi) PCR following broth pre-enrichment. Results: The numbers of samples in which B. pseudomallei was detected were 42, 10, 7, 6, 6 and 84, respectively. However, two samples were positive by the consensus method but negative by conventional culture, and one sample was negative by PCR following enrichment although B. pseudomallei was isolated by the conventional culture method. Conclusions/Discussion: The results show that no single method will detect all environmental samples that contain B. pseudomallei. People conducting environmental surveys for this organism should be aware of the possibility of false-negative results using the consensus culture method. An approach that entails screening using PCR after enrichment, followed by the evaluation of a range of different culture methods on PCR-positive samples to determine which works best in each setting, is recommended.

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Preliminary Molecular Studies of the First Report of Burkholderia pseudomallei Isolation from Soil Collected in the Amapá State, in Northern Brazil

Cited by 4 publications

References 17 publications

Melioidosis in South America

Melioidosis in South America

Biology of the non-parasitic phase of the cattle tick Rhipicephalus (Boophilus) microplus in an area of Amazon influence

Evaluation of consensus method for the culture of Burkholderia pseudomallei in soil samples from Laos

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