Preliminary Phytochemical Screening and Infra-Radiation Test of Crude the Leaves of Nothophanax scutellarium Merr., Moringa oleifera and Piper betle L.

Sriyanti, Cut; Siregar, Tongku Nizwan; Mudatsir, Mudatsir; Gani, Azhari

doi:10.52711/0974-360x.2022.00303

Cited by 2 publications

(4 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…N. scutellarium leaves were obtained from Banda Aceh and Aceh Besar, Indonesia. The extraction protocol followed the suggestion from a previous study [ 14 ]. Briefly, air-dried powder of each plant (300 g) was macerated with 3 L ethanol 70% for seven days at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial and anti-inflammatory activities of Nothopanax scutellarium, Moringa oleifera and Piper betle extracts on staphylococcal mastitis animal model

Sriyanti,

Siregar,

Mudatsir

et al. 2023

Narra J

View full text Add to dashboard Cite

Inappropriate and prolonged administration of antibiotics in mastitis could cause antibiotic resistance and herbal treatment might could be one alternative treatment. Nothopanax scutellarium, Moringa oleifera, and Piper betle are medicinal plants that contain various active compounds, including antibacterial and anti-inflammatory agents, but their potentials in treating mastitis are not well understood. The aim of this study was to assess the effectiveness of those plants against mastitis in rabbit model induced by Staphylococcus aureus. A total of 25 lactating rabbits (Oryctolagus cuniculus) weighing 3.0±0.4 kg were grouped into five groups: healthy control; mastitis control, and three treatment groups (Nothopanax scutellarium, Moringa oleifera, and Piper betle). Except the healthy control group, all animals were inoculated with 0.15 mL of Staphylococcus aureus containing 1.5x107colony forming unit (CFU)/mL on eight days after giving birth. The extract was administered orally after four hours Staphylococcus aureus inoculation at a dose of 50 mg/kg body weight, twice a day for five consecutive days. The number of bacteria in the milk and the level of serum interleukin 6 (IL-6) were measured and histopathological examination of mammary gland tissues were analyzed. The log number of total plate count of Staphylococcus aureus indicated that all extract groups had significant lower of bacterial logs compared to mastitis control (all comparisons had p<0.05) with the lowest was found in Piper betle group, followed by Nothopanax scutellarium and Moringa oleifera. The enzyme-linked immunosorbent assay (ELISA) results showed that all ethanolic extract groups had significantly lower levels of IL-6 compared to the mastitis control (all comparisons had p<0.05). The histopathologyassessment suggested that extract groups had lower infiltration of inflammatory cells such as lymphocytes and macrophages in alveoli compared to the mastitis control group. In conclusion, all three extracts contained antibacterial and anti-inflammatory activities and Piper betle had the most effective in reducing bacterial growth and IL-6 level compared to others.

show abstract

Section: Methodsmentioning

confidence: 99%

Antibacterial and anti-inflammatory activities of Nothopanax scutellarium, Moringa oleifera and Piper betle extracts on staphylococcal mastitis animal model

Sriyanti,

Siregar,

Mudatsir

et al. 2023

Narra J

View full text Add to dashboard Cite

show abstract

“…The finely blended result was sieved and placed into clean jars. Simplicia characterization was conducted following the guidelines of Sriyanti et al (2022).…”

Section: Preparation Of Simpliciamentioning

confidence: 99%

“…Phytochemical screening for identifying flavonoids, terpenoids, and steroids was conducted following the guidelines of Sriyanti et al (2022). The identification of alkaloids and saponins was performed according to the guidelines of Heinrich et al (2022), while the identification of tannins was carried out by Sriyanti et al (2022).…”

Section: Phytochemical Analysis Of Methanol Extracts From Tubers Ant ...mentioning

confidence: 99%

See 1 more Smart Citation

Identification, screening, and analysis of secondary metabolite content in methanol extracts of ant nests plant tubers from Aceh, Indonesia

ROSLIZAWATY,

GHOLIB,

RAHMI

et al. 2024

Biodiversitas

View full text Add to dashboard Cite

Abstract. Roslizawaty R, Gholib G, Rahmi N, Khairan K, Idroes R, Syafruddin S, Abrar M. 2023. Identification, screening, and analysis of secondary metabolite content in methanol extracts of ant nests plant tubers from Aceh, Indonesia. Biodiversitas 24: 6934-6941. This study aims to identify the chemical components obtained from extracting and partitioning methanol extracts from ant nest tubers (Myrmecodia sp.) originating from Aceh Jaya and Aceh Besar. These extracts were identified using phytochemical screening, Fourier Transform Infrared (FT-IR), and Gas Chromatography-Mass Spectroscopy (GC-MS) methods. The identification results showed that the ant nest plant from Aceh Jaya belongs to the species Myrmecodia tuberosa Jack. The ant nest plant from Aceh Besar belongs to the species Hydnophytum formicarum Jack. Phytochemical screening results showed that the methanol extract from Aceh Jaya's ant nest tubers contained flavonoids, tannins, saponins, and terpenoids, while Aceh Besar's ant nest tubers contained flavonoids, tannins, terpenoids. GC-MS analysis revealed that the major compound found in the methanol extract from Aceh Jaya's ant nest tubers was 2H-oxecin-2-one,3,4,7,8,9,10-hexahydro-4-hydroxy-10-methyl-,[4S-(4R*,5E,10S*)]- constituting 50.63% of the extract. In comparison, the primary compound in the methanol extract from Aceh Besar was resorcinol at 17.92%. FT-IR analysis indicated that the FT-IR spectra of the methanol extracts from Aceh Jaya's ant nest tubers contained O-H, C-H, C=O, C=C alkene, and C=C aromatic groups. At the same time, methanol extracts from Aceh Besar's ant nest tubers also contained O-H, C-H, C=O, C=C alkene, and C=C aromatic groups.

show abstract

Preliminary Phytochemical Screening and Infra-Radiation Test of Crude the Leaves of Nothophanax scutellarium Merr., Moringa oleifera and Piper betle L.

Cited by 2 publications

References 28 publications

Antibacterial and anti-inflammatory activities of Nothopanax scutellarium, Moringa oleifera and Piper betle extracts on staphylococcal mastitis animal model

Antibacterial and anti-inflammatory activities of Nothopanax scutellarium, Moringa oleifera and Piper betle extracts on staphylococcal mastitis animal model

Identification, screening, and analysis of secondary metabolite content in methanol extracts of ant nests plant tubers from Aceh, Indonesia

Contact Info

Product

Resources

About